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Absorption Kinetics and Efflux of Chloride and Sodium in the Roots of Four Grape Genotypes (Vitis L.) Differing in Salt Tolerance

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Abstract

Grapevine is classified as a moderately sensitive plant to salinity. In this study, two sensitive (GhezelUzum and Shirazi) and two tolerant (H6 and Gharashani) grape genotypes, based on screening experiments under salinity, were selected. Hydroponically grown plants were treated with different concentrations of NaCl. Cl− and Na+ contents were significantly increased in different plant organs of all the genotypes. Under salinity, the roots of the tolerant genotypes accumulated higher Cl− content, compared to the sensitive ones. Cl− content in the shoots of sensitive genotypes was threefold higher than the tolerant ones. The absorption kinetics of Cl− and Na+ under salinity was fitted to the Michaelis–Menten equation. Cl− uptake rate in roots of sensitive genotypes was 2.84 times higher than the tolerant ones. And, Na+ uptake ratio was 1.98. There was significant positive correlation between Cl− and Na+ accumulation in all the genotypes under salinity, and the uptake ratio of Na+ was close to 4. Our studied sensitive genotypes showed a higher cytoplasmic and vacuolar Cl− and Na+ values, compared to the tolerant ones. The difference in salt tolerance among the genotypes can be related to different Cl− and Na+ influx, transport and efflux in the sensitive and tolerant genotypes.

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References

  • Abbaspour N, Kaiser B, Tyerman S (2013) Chloride transport and compartmentation within main and lateral roots of two grapevine rootstocks differing in salt tolerance. Trees 27:1317–1325

    Article  Google Scholar 

  • Abbaspour N, Kaiser B, Tyerman S (2014) Root apoplastic transport and water relations cannot account for differences in Cl− transport and Cl−/NO3 − interactions of two grapevine rootstocks differing in salt tolerance. Acta Physiol Plant 36(3):687–698

    Article  Google Scholar 

  • Ahn SJ, Shin R, Schachtman DP (2004) Expression of KT/KUP genes in Arabidopsis and the role of root hairs in K+ uptake. Plant Physiol 134:1135–1145

    Article  Google Scholar 

  • Blumwald E, Aharon GS, Apse MP (2000) Sodium transport in plant cells. Biochim Biophys Acta 1465:140–151

    Article  Google Scholar 

  • Britto DT, Kronzucker HJ (2006) Futile cycling at the plasma membrane: a hallmark of low-affinity nutrient transport. Trends Plant Sci 11:529–534

    Article  Google Scholar 

  • Brumos J, Colmenero-Flores J, Conesa A, Izquierdo P, Sanchez G, Iglesias D, Lopez-Climent M, Gómez-Cadenas A, Talon M (2009) Membrane transporters and carbon metabolism implicated in chloride homeostasis differentiate salt stress responses in tolerant and sensitive Citrus rootstocks. Funct Integr Genomics 9:293–309

    Article  Google Scholar 

  • Chartzoulakis K, Loupassaki M, Bertaki M, Androulakis I (2002) Effects of NaCl salinity on growth, ion content and CO2 assimilation rate of six olive cultivars. Sci Hortic 96:235–247

    Article  Google Scholar 

  • Cubero-Font P, Maierhofer T, Jaslan J, Rosales Miguel A, Espartero J, Díaz-Rueda P, Müller Heike M, Hürter A-L, Al-Rasheid Khaled AS, Marten I, Hedrich R, Colmenero-Flores José M, Geiger D (2016) Silent S-type anion channel subunit SLAH1 gates SLAH3 open for chloride root-to-shoot translocation. Curr Biol 26(16):2213–2220

    Article  Google Scholar 

  • Davenport R, James RA, Zakrisson-Plogander A, Tester M, Munns R (2005) Control of sodium transport in durum wheat. Plant Physiol 137:807

    Article  Google Scholar 

  • Downton WJS (1977) Chloride accumulation in different species of grapevines. Sci Hort 7:249–253

    Article  Google Scholar 

  • Elphick CH, Sanders D, Maathuis FJM (2001) Critical role of divalent cations and Na+ efflux in Arabidopsis thaliana salt tolerance. Plant Cell Environ 24:733–740

    Article  Google Scholar 

  • FAO (2000) Production year book. Food and Agriculture Organization of the United Nations, Rome

    Google Scholar 

  • Fisarakis I, Chartzoulakis K, Stavrakas D (2001) Response of Sultana vines (V. vinifera L.) on six rootstocks to NaCl salinity exposure and recovery. Agric Water Manage 51:13–27

    Article  Google Scholar 

  • Fisarakis I, Nikolaou N, Tsikalas P, Therios I, Stavrakas D (2004) Effect of salinity on concentration of potassium, calcium, magnesium, phosphorus and nitrate-nitrogen in Thompson seedless grapevine. J Plant Nutr 27:2117–2134

    Article  Google Scholar 

  • Greenway H, Munns R (1980) Mechanisms of salt tolerance in non-halophytes. Ann Rev Plant Physiol 31:149–190

    Article  Google Scholar 

  • Kronzucker HJ, Britto DT (2011) Sodium transport in plants: a critical review. New Phytol 189:54–81

    Article  Google Scholar 

  • Li B, Byrt C, Qiu J, Baumann U, Hrmova M, Evrard A, Johnson AAT, Birnbaum KD, Mayo GM, Jha D, Henderson SW, Tester M, Gilliham M, Roy SJ (2016) Identification of a stelar-localized transport protein that facilitates root-to-shoot transfer of chloride in arabidopsis. Plant Physiol 170(2):1014–1029

    Article  Google Scholar 

  • Li B, Tester M, Gilliham M (2017) Chloride on the move. Trends Plant Sci. https://doi.org/10.1016/j.tplants.2016.12.004

    Article  Google Scholar 

  • Marschner H (1995) Mineral nutrition in higher plants, 2nd edn. Academic Press, London

    Google Scholar 

  • Massa D, Mattson NS, Lieth HJ (2009) Effects of saline root environment (NaCl) on nitrate and potassium uptake kinetics for rose plants: a Michaelis–Menten modelling approach. Plant Soil 318:101–115

    Article  Google Scholar 

  • Mohammadkhani N, Heidari R, Abbaspour N, Rahmani F (2013) Comparative study of salinity effects on ionic balance and compatible solutes in nine Iranian table grape (Vitis vinifera L.) genotypes. J Int Sci Vig Vin 47(2):99–114

    Google Scholar 

  • Mohammadkhani N, Heidari R, Abbaspour N, Rahmani F (2014) Evaluation of salinity effects on ionic balance and compatible solute contents in nine grape (Vitis L.) genotypes. J Plant Nutr 37:1817–1836

    Article  Google Scholar 

  • Munns RA (2005) Genes and salt tolerance: bringing them together. New Phytol 167:645–663

    Article  Google Scholar 

  • Oertli JJ (1968) Extracellular salt accumulation, a possible mechanism of salt injury in plants. Agrochimica 12:461–469

    Google Scholar 

  • Rains DW, Epstein E (1967) Sodium absorption by barley roots: role of dual mechanisms of alkali cation transport. Plant Physiol 42:314–318

    Article  Google Scholar 

  • Rodriguez-Navarro A, Rubio F (2006) High-affinity potassium and sodium transport systems in plants. J Exp Bot 57:1149–1160

    Article  Google Scholar 

  • Savvas D, Mantzos N, Barouchas PE, Tsirogiannis IL, Olympios C, Passam HC (2007) Modelling salt accumulation by a bean crop grown in a closed hydroponic system in relation to water uptake. Sci Hort 111:311–318

    Article  Google Scholar 

  • Schubert S, Lauchli A (1990) Sodium exclusion mechanisms at the root surface of two maize cultivars. Plant Soil 123:25–209

    Article  Google Scholar 

  • Shabala SN, Shabala L, van Volkenburgh E (2003) Effect of calcium on root development and root ion fluxes in salinized barley seedlings. Funct Plant Biol 30:507–514

    Article  Google Scholar 

  • Storey R, Schachtman DP, Thomas MR (2003) Root structure and cellular chloride, sodium and potassium distribution in salinized grapevines. Plant Cell Environ 26:789–800

    Article  Google Scholar 

  • Teakle NL, Tyerman SD (2010) Mechanisms of Cl− transport contributing to salt tolerance. Plant Cell Environment 33:566–589

    Article  Google Scholar 

  • Tester M, Davenport R (2003) Na+ tolerance and Na+ transport in higher plants. Ann Bot 91:503–527

    Article  Google Scholar 

  • Tregeagle JM, Tisdall JM, Tester M, Walker RR (2010) Cl– uptake, transport and accumulation in grapevine rootstocks of differing capacity for Cl– exclusion. Funct Plant Biol 37:665–673

    Article  Google Scholar 

  • Tyerman SD, Skerrett IM (1999) Root ion channels and salinity. Sci Hort 78:175–235

    Article  Google Scholar 

  • Walker RR, Blackmore DH, Clingeleffer PR, Correll RL (2004) Rootstock effects on salt tolerance of irrigated field-grown grapevines (Vitis vinifera L. cv. Sultana) 2. Ion concentrations in leaves and juice. Aust J Grape Wine Res 10:90–99

    Article  Google Scholar 

  • White PJ, Broadley MR (2001) Chloride in soils and its uptake and movement within the plant. Ann Bot 88:967–988

    Article  Google Scholar 

  • Yeo AR, Flowers TJ (1982) Accumulation and localisation of sodium ions within the shoots of rice (Oryza sativa) varieties differing in salinity resistance. Physiol Plant 56:343–348

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Shahid Bakeri High Education Center of Miandoab, Urmia University, Urmia, Iran

    Nayer Mohammadkhani

  2. Department of Biology, Faculty of Science, Urmia University, Urmia, Iran

    Nasser Abbaspour

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  1. Nayer Mohammadkhani
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  2. Nasser Abbaspour
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Correspondence to Nayer Mohammadkhani.

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Mohammadkhani, N., Abbaspour, N. Absorption Kinetics and Efflux of Chloride and Sodium in the Roots of Four Grape Genotypes (Vitis L.) Differing in Salt Tolerance. Iran J Sci Technol Trans Sci 42, 1779–1793 (2018). https://doi.org/10.1007/s40995-017-0445-0

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  • DOI: https://doi.org/10.1007/s40995-017-0445-0

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