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Green Synthesis, Characterization, and Application of Ascophyllum Nodosum Silver Nanoparticles

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Abstract

Purpose

Green nanotechnology as a field has emerged and gained popularity amongst biologists due to its cost-effective and environment-friendly advancements. The most preferred is the biological method which involves plants and their extracts.

Methods

The silver nanoparticles were synthesized by a sunlight-driven aqueous extract (AE) of whole plant powder of Ascophyllum nodosum. Advanced techniques like high-resolution scanning electron microscopy (HRSEM), energy dispersive X-ray (EDX), high-resolution transmission electron microscopy (HRTEM), and particle size analysis were used to determine the nature of nanoparticles. Antioxidant, anti-fungal, and anti-leishmanial activities were evaluated.

Result

The techniques confirmed the formation of spherical particles of the desired range of size. Silver nanoparticles exhibited a much greater DPPH (2,2-diphenyl-1-picryl-hydrazine-hydrate) radical scavenging activity which was almost six to seven folds more than that exhibited by the AE alone. The anti-leishmanial and cytotoxic activities were evaluated on Leishmania donovani promastigote and amastigote.

Conclusion

The synthesized AgNPs showed remarkable DPPH radical scavenging ability owing to their antioxidant properties. The anti-leishmanial activity was exceptionally viable in both AE and AgNPs. The findings all together support the tendency of Ascophyllum nodosum to efficiently synthesized AgNPs which could be utilized for its anti-leishmanial properties.

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Acknowledgements

We are very much thankful to scientists from Canada for sharing the processed sample of Ascophylum nodosum. We are also thankful to the staff of the Central Instrument Facility, Indian Institute of Technology, BHU, and Department of Botany, Banaras Hindu University Varanasi.

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Authors and Affiliations

  1. Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology, Banaras Hindu University, Varanasi, India

    Sunil Kumar Mishra, Saket Sinha & Amit Kumar Singh

  2. Molecular Science Lab, National Institute of Immunology, New Delhi, India

    Prabhat Upadhyay

  3. Department of Biotechnology, Jamia Hamdard University, New Delhi, India

    Diya Kalra

  4. Department of Botany, MMV, Banaras Hindu University, Varanasi, India

    Pradeep Kumar & Kavindra Nath Tiwari

  5. Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, India

    Rajan Singh & Rakesh Kumar Singh

  6. School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, India

    Arvind Kumar & Alok Tripathi

  7. Cell and Neurobiology lab Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, India

    Brijesh Singh Chauhan & S. Srikrishna

Authors
  1. Sunil Kumar Mishra
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  2. Saket Sinha
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  13. S. Srikrishna
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Contributions

Conceptualization and methodology: Sunil Kumar Mishra, Kavindra Nath Tiwari, Rakesh Singh, Sri Krishna, and Alok Tripathi; experimentation, data curation, investigation: Saket Sinha, Amit Kumar Singh, Pradeep Kumar, Rajan Singh, Arvind Kumar, Brijesh Singh Chauhan; writing—original draft, review, and editing: Prabhat Upadhyay and Diya Kalra; manuscript checking: SKM and KNT. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Sunil Kumar Mishra.

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The authors carried out no animal or human studies for this article.

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The authors declare no competing interests.

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Key Points

1. Aqueous extract of A. nodosumas served as a capping and stabilizing agent for photo-induced synthesis of AgNPs of 30–60 nm in size.

2. The tendency of Ascophyllum nodosum to efficiently synthesize AgNPs which could be utilized for its anti-leishmanial properties.

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Mishra, S.K., Sinha, S., Singh, A.K. et al. Green Synthesis, Characterization, and Application of Ascophyllum Nodosum Silver Nanoparticles. Regen. Eng. Transl. Med. 9, 518–532 (2023). https://doi.org/10.1007/s40883-023-00298-x

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  • DOI: https://doi.org/10.1007/s40883-023-00298-x

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