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Approaches to Optimize Stem Cell-Derived Cardiomyocyte Maturation and Function

  • Stem Cell Switches and Regulators (K Hirschi and N Genet, Section Editors)
  • Published:
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Abstract

Purpose of Review

Human induced pluripotent stem cells (hiPSC) have become one of the most promising cell biology tools over the past decade. With the potential to be differentiated into diverse human cell types, their use ranges from disease modeling and drug development, to future personalized cell therapies and regenerative medicine. One of the ongoing challenges of differentiating hiPSC is to obtain cells in vitro with a mature phenotype as close as possible to human adult cells. In this context, maturation of hiPSC-derived cardiomyocytes (hiPSC-CM) is a challenge where diverse regulatory factors must be considered to obtain functionally mature cells. This review provides an overview of the current protocols for hiPSC-CM maturation.

Recent Findings

The most promising maturation protocols include 3D tissue engineering, electrical stimulation, hormonal treatment, co-culture with non-myocyte cells, metabolic modifiers, and modification of the microenvironment, to better reproduce the native cardiac environment.

Summary

Each technique implements some improvements in hiPSC-CM maturation, leading to improved structure and function, and helping to unravel the puzzle of the heart maturation process. Future studies will need to combine different methods to further improve cardiomyocyte maturation to pave the way for heart regenerative medicine.

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Dr Guillaume Gilbert received funding from a FWO postdoctoral fellowship.

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  1. Laboratory of Experimental Cardiology, Department of Cardiovascular Sciences, KU Leuven, Leuven, Belgium

    Guillaume Gilbert

  2. EA 4324 ORPHY, UFR Sciences Et Techniques, Université de Brest, 6 avenue Victor Le Gorgeu, F-29238, Brest, France

    Guillaume Gilbert

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Gilbert, G. Approaches to Optimize Stem Cell-Derived Cardiomyocyte Maturation and Function. Curr Stem Cell Rep 7, 140–160 (2021). https://doi.org/10.1007/s40778-021-00197-3

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