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Evidence for decreased expression of APPL1 associated with reduced insulin and adiponectin receptors expression in PCOS patients

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An Erratum to this article was published on 24 June 2016

Abstract

Purpose

To investigate the expression of Adaptor protein containing a PH domain, PTB domain and leucine zipper motif 1 (APPL1), insulin receptor (INSR), adiponectin and adiponectin receptors (adipoR1 and R2) and their possible associations in granulosa cells (GCs) of 22 polycystic ovary syndrome (PCOS) women compared to the 22 non-PCOS controls with normal ovulatory function matched for BMI (body mass index).

Methods

In this study, 44 infertile women aged 18-40 years undergoing in vitro fertilization (IVF) protocol were recruited. After follicular fluid collection, GCs were isolated and then purified with MACS (Micro Beads conjugated to monoclonal anti-human CD45 antibodies). RNA was extracted from GCs and quantitative real-time PCR (qRT-PCR) was performed to assess APPL1 gene expression.

Results

Expression of APPL1, insulin receptor and adiponectin system genes was significantly decreased in PCOS group compared to the controls.

Conclusions

Reduction of APPL1, insulin receptor and adiponectin system genes in GCs could be involved in the development of PCOS.

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Acknowledgments

This work was supported by Research and Technology Deputy of Hamadan University of Medical Sciences as a M.S thesis. The authors thank the Endometrium and Endometriosis Research Center for clinical evaluation of patients and also the Research Center for Molecular Medicine, Hamadan university of Medical Sciences for technical assistance.

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Authors and Affiliations

  1. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    R. Dehghan, M. Saidijam & N. Shabab

  2. Cellular and Molecular Research Center, Faculty of Advanced Technology in Medicine, Anatomy Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    M. Mehdizade

  3. Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

    M. Yavangi & T. Artimani

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  1. R. Dehghan
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  2. M. Saidijam
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Correspondence to T. Artimani.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

In accordance with the ethical standards of research, study protocol was approved by Hamadan University of Medical Sciences Ethics Committee (number: 16.35.9.5431).

Informed consent

All the participants were fully justified for the study and a written informed consent was obtained from each of them.

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Dehghan, R., Saidijam, M., Mehdizade, M. et al. Evidence for decreased expression of APPL1 associated with reduced insulin and adiponectin receptors expression in PCOS patients. J Endocrinol Invest 39, 1075–1082 (2016). https://doi.org/10.1007/s40618-016-0468-y

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  • DOI: https://doi.org/10.1007/s40618-016-0468-y

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