Abstract
Taxus baccata produces an anticancer drug. Taxol but its natural production is very poor which has resulted into excessive cutting and due to this the plant has entered the red data book as an endangered species. The demand for Taxol is high and in order to increase the production of Taxol, both conservation and alternative methods of production are required. Macro-propagation of the endangered Himalayan yew, through yew cuttings was tried in this study. The yew cuttings were dipped in Auxin solution (IAA, IBA, NAA in range of 250 mg/l, 500 mg/l, 1000 mg/l, 2000 mg/l and 5000 mg/l concentration) individually, then maintained in auxin fortified soil at 25 ± 1 °C and 65% humidity in 16/8 h day-night photoperiodic cycle. The effect on overall growth and morphology of the plant was studied in each auxin separately in the mentioned concentration. The initial results of four weeks suggested an increase in plant height where plant height in IBA increased by 86% followed by IAA that increased by 69% and increase in plant height in NAA was 56% respectively. The increase of plant height between 4 and 16 weeks depicted NAA (81%) at 500 mg/l concentration, IAA (117%) at 1000 mg/l and IBA (127%) were best for the sustainability of healthy plants. The results achieved over sixteen weeks indicated pronounced adventitious roots formation from yew cuttings with overall a difference up to 95% growth in plant height in addition to appearance of 50% more leaves when compared to control.
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The author is thankful to the Department of Biotechnology, Sharda University for providing the space for research work.
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PS completed the bench work and initially drafted the manuscript. VBS planned, supervised and finalized the manuscript for publication.
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Sahai, P., Sinha, V.B. In vitro culturing of Taxus baccata sub spp. wallichiana via fortified yew cuttings in tropical climate. Plant Physiol. Rep. 27, 419–427 (2022). https://doi.org/10.1007/s40502-022-00673-w
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DOI: https://doi.org/10.1007/s40502-022-00673-w