Abstract
Ardisia crenata Sims var. bicolor has high ornamental and medical value. To protect wild germplasm resources and obtain more ardicrenin, a plant regeneration system was established via calli, and ardicrenin content was quantified by reversed-phase high-performance liquid chromatography (RP-HPLC). The results showed that a Murashige and Skoog (MS) medium with 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg L−1) and kinetin (KT, 0.01 mg L−1) was suitable for callus induction, with an induction rate of 95.31 ± 1.62%; a MS medium with 2,4-D (0.5 mg L−1) and 6-benzylaminopurine (6-BA, 0.2 mg L−1) was suitable for callus multiplication, with a multiplication coefficient of 359.26; a MS medium with 6-BA (1.0 mg L−1) and 1-naphthaleneacetic acid (NAA, 0.5 mg L−1) was suitable for callus differentiation, with a differentiation rate of 89.36 ± 1.39%; and 1/2 MS with indole-3-butyric acid (IBA, 0.2 mg L−1) was suitable for rooting, with a rooting rate of 93.74 ± 1.38%. Additionally, garden soil: vermiculite: perlite (v: v: v = 1:1:1) was appropriate for acclimatization and transplanting, with a survival rate of 87.38%. RP-HPLC analysis revealed that the ardicrenin content in one-month-old plantlets was higher than that in one-year-old plants and was nearly the same as the content observed in three-year-old plants and two-month-old transplanted plantlets, especially in the roots (17.69 mg g−1 DW, 1.77% DW). In summary, a rapid propagation system for A. crenata Sims var. bicolor was successfully established for the first time, and the roots of 1-month-old plantlets were used to effectively obtain ardicrenin.
Data availability statements
The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy.
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Acknowledgements
This work was supported by the Guangxi Universities Young and Middle-aged Scientific Research Basic Ability Improvement Project China (No. 2022KY0583), the High-level Talent Research Start-Up Fund of Yulin Normal University, Guangxi, China (No. G2019ZK43), and the College Students’ Innovation Entrepreneurship Training Plan Program (Nos. 202110606158, S202210606128). Special thanks to the American Journal Experts-AJE Company for editing the language in the original manuscript.
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JH and FL designed the research. CYM, HCL, LJK, SCX, HYZ, and SRP conducted the experiments. ZL, XLY, and LL analyzed the data. ZL wrote the manuscript, JH and FL provided helpful suggestions and helped revise the manuscript carefully during the course of major revisions and minor edits. All authors read and approved the manuscript.
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Lin, Z., Yang, X., Li, L. et al. A rapid and effective method for obtaining ardicrenin from Ardisia crenata Sims var. bicolor plants. Braz. J. Bot 46, 505–512 (2023). https://doi.org/10.1007/s40415-023-00909-5
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DOI: https://doi.org/10.1007/s40415-023-00909-5