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Transcriptome profiling reveals candidate genes associated with cold stress in mulberry

  • Genetics & Evolutionary Biology - Original Article
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Abstract

Low temperatures limit crop growth, yield, and quality in cold areas, and cold tolerance is the only strategy for plants to survive in those areas. However, mulberry’s (Morus alba L.) molecular regulatory mechanisms in response to cold remain poorly understood. In this report, four cDNA libraries were built from two groups, the cold-treated group and the control group, and evaluated by RNA-Seq analysis. A total of 3593 differential expression genes (DEGs) were identified from 20,279 unigenes. Among these, 2337 up-regulated and 1256 down-regulated DEGs were identified under this standard (corrected p value < 0.05, log2fold change > 0.0). Most of these DEGs are involved in plant hormone signal transduction, MAPK signaling pathway, ubiquinone, and other terpenoid-quinone biosynthesis, nitrogen metabolism, and biosynthesis of secondary metabolites. A series of candidate genes related to cold stress were screened out and discussed based on these results. Furthermore, the expression changes of 8 genes under cold stress from RNA-Seq analysis were further validated by real-time quantitative polymerase chain reaction. This research reflects Morus alba L. transcriptome data and may help elucidate the cold response mechanisms of mulberry trees and would be useful in breeding cold-tolerant mulberry plants.

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Abbreviations

DEGs:

Differential expression genes

cDNA:

Complementary deoxyribonucleic acid

qRT-PCR:

Quantitative real-time polymerase chain reaction

PCR:

Polymerase chain reaction

KEGG:

Kyoto Encyclopedia of Genes and Genomes

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Acknowledgements

This work was supported by Sericulture Industry Technology in China Agriculture Research System (CARS-18-ZJ0207), Guangxi innovation driven development project (AA19182012-2), Open Program of Key Laboratory of Silkworm and Mulberry Genetic Improvement Ministry of Agriculture, China (KL201906), the Crop Germplasm Resources Protection Project of the Agriculture Ministry (111721301354052026), and National Infrastructure for Crop Germplasm Resources (NICGR-43).

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  1. Acheampong Adolf, Li Liu and Michael Ackah have contributed equally to this work.

Authors and Affiliations

  1. School of Biotechnology, Jiangsu University of Science and Technology, Nanxu Road, Sibaidu, Zhenjiang, 212018, Jiangsu, People’s Republic of China

    Acheampong Adolf, Li Liu, Michael Ackah, Yang Li, Qiuxia Du, Danyan Zheng, Peng Guo, Yisu Shi & Weiguo Zhao

  2. Guangxi Sericultural Research Institute, Guangxi Zhuang Autonomous Region, Nanning, 530007, People’s Republic of China

    Qiang Lin & Changyu Qiu

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  1. Acheampong Adolf
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Contributions

AA, LL, LQ, and ZW conceived and designed research. AA, LY, and DQ conducted experiments. MA, ZD, and GP contributed new reagents or analytical tools. AA, SY, and QC analyzed data. AA and LL wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Weiguo Zhao.

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Adolf, A., Liu, L., Ackah, M. et al. Transcriptome profiling reveals candidate genes associated with cold stress in mulberry. Braz. J. Bot 44, 125–137 (2021). https://doi.org/10.1007/s40415-020-00680-x

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