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Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice

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Abstract

The TIFY family is a novel plant-specific gene family involved in the regulation of diverse plant-specific biologic processes, such as development and responses to phytohormones, in Arabidopsis. However, there is limited information about this family in monocot species. This report identifies 20 TIFY genes in rice, the model monocot species. Sequence analysis indicated that rice TIFY proteins have conserved motifs beyond the TIFY domain as was previously shown in Arabidopsis. On the basis of their protein structures, members of the TIFY family can be divided into two groups. Transcript level analysis of OsTIFY genes in tissues and organs revealed different tempo-spatial expression patterns, suggesting that expression and function vary by stage of plant growth and development. Most of the OsTIFY genes were predominantly expressed in leaf. Nine OsTIFY genes were responsive to jasmonic acid and wounding treatments. Interestingly, almost all the OsTIFY genes were responsive to one or more abiotic stresses including drought, salinity, and low temperature. Over-expression of OsTIFY11a, one of the stress-inducible genes, resulted in significantly increased tolerance to salt and dehydration stresses. These results suggest that the OsTIFY family may have important roles in response to abiotic stresses. The data presented in this report provide important clues for further elucidating the functions of the genes in the OsTIFY family.

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Abbreviations

ABA:

Abscisic acid

GA:

Gibberellic acid

JA:

Jasmonic acid

Jas:

JASMONATE-ASSOCIATED

JAZ:

JASMONATE ZIM-DOMAIN

SA:

Salicylic acid

ZIM:

Zinc-finger protein expressed in inflorescence meristem

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Acknowledgments

We thank our colleagues Lei Wang for providing Ghd7-CFP construct, and Jian Xu for providing the pM999 vector, and Yubing He for assistance in proline content measurement. This work was supported by grants from the National Special Key Project of China on Functional Genomics of Major Plants and Animals, the National Program on the Development of Basic Research, the National Natural Science Foundation of China, the Ministry of Education of China (NO 707045), and the EU FP6 INCO-MPC2 project CEDROME (INCOCT-2005-015468).

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  1. National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China

    Haiyan Ye, Hao Du, Ning Tang, Xianghua Li & Lizhong Xiong

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  1. Haiyan Ye
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Correspondence to Lizhong Xiong.

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Ye, H., Du, H., Tang, N. et al. Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice. Plant Mol Biol 71, 291–305 (2009). https://doi.org/10.1007/s11103-009-9524-8

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