Abstract
This study investigated the effects of cerium on the regeneration and biosynthesis of ascorbate and glutathione in the leaves of turf grass Poa pratensis seedlings under copper (Cu) stress. The results showed that Cu stress increased the activities of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), glutathione reductase (GR), l-galactono-1,4-lactone dehydrogenase (GalLDH) and gamma-glutamylcysteine synthetase (γ-ECS), and the contents of reduced ascorbic acid (AsA), reduced glutathione (GSH), total ascorbate and total glutathione. However, Cu stress markedly increased the malondialdehyde content and electrolyte leakage and decreased the fresh and dry weights. Pretreatment with cerium (Ce(NO3)3) enhanced the activities of APX, GR, DHAR, GalLDH and γ-ECS, and the contents of AsA, GSH, total ascorbate and total glutathione under Cu stress. Meanwhile, pretreatment with Ce(NO3)3 decreased the malondialdehyde content and electrolyte leakage and increased the fresh and dry weights under Cu stress. Our results suggested that Ce(NO3)3 alleviated oxidative damage by regulating the metabolism of ascorbate and glutathione under Cu stress, and has important role in the acquisition of Cu tolerance in P. pratensis seedlings. Therefore, Ce(NO3)3 can be used as a regulator to improve the Cu tolerance of P. pratensis.
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This study was funded by the “Important Scientific Research Project of Henan Institute of Science and Technology (2011010)”.
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Liu, R., Shan, C., Gao, Y. et al. Cerium improves the copper tolerance of turf grass Poa pratensis by affecting the regeneration and biosynthesis of ascorbate and glutathione in leaves. Braz. J. Bot 39, 779–785 (2016). https://doi.org/10.1007/s40415-015-0246-7
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DOI: https://doi.org/10.1007/s40415-015-0246-7