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Comparative karyotypic analysis and cytotaxonomy in the Alstroemeria ligtu L. (Alstroemeriaceae) complex of Chile

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Abstract

Due to the variability in morphology and color of the flowers as well as the excellent post-harvest durability of Alstroemeria, many species are cultivated as ornamental and cut flowers. Alstroemeria ligtu L., endemic to Chile, has also been a source of artificial hybridizations for development of new cultivars. Cytogenetic studies provide clues for possible breeding programs and taxonomically useful data to clear up infraspecific relationships of Chilean native populations. Three subspecies are recognized within A. ligtu: subsp. ligtu, subsp. splendens Muñoz-Schick, and subsp. simsii (Sprengel) Bayer. The objective of this paper is to characterize karyotypically these subspecies and to clarify their taxonomic status. Fourteen populations were collected throughout its distributional range. Chromosome number, karyotypic formulae, karyotypes, ideograms, intrachromosomal asymmetry index MCA, and interchromosomal asymmetry index CVCL were calculated. All studied populations showed 2n = 2x = 16 chromosomes. Subspecies ligtu and simsii are clearly differentiated from each other in MCA and together from subsp. splendens with CVCL. Intrachromosomal asymmetry index revealed two populational groups within subsp. splendens. These populations also differ in karyotypes formulae, habitat, soil type, and distribution. We concluded that a fourth subspecies should be described from populations located in the lower part of the cordillera de los Andes in the Region of Maule. Populations of higher elevations correspond to those already described as subsp. splendens.

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References

  • Aagesen L, Sanso M (2003) The phylogeny of the Alstroemeriaceae, based on morphology, rps16 Intron, and rbcL sequence data. Syst Bot 28:47–69

    Google Scholar 

  • Aker S, Healy W (1990) The phytogeography of the genus Alstroemeria. Herbertia 462:76–87

    Google Scholar 

  • Baeza C, Ruiz E (2011) Alstroemeria hookeri Lodd. subsp. sansebastiana C.M. Baeza & E. Ruiz, nueva para la flora de Chile. Gayana Bot 68:313–315

    Article  Google Scholar 

  • Baeza M, Schrader O, Ruiz E, Negritto M (2006) Análisis comparativo del cariotipo en poblaciones de Alstroemeria ligtu subsp. ligtu y A. ligtu subsp. simsii (Alstroemeriaceae) de Chile. Darwiniana 44:313–318

    Google Scholar 

  • Baeza C, Schrader O, Budahn H (2007) Characterization of geographically isolated accessions in five Alstroemeria L. species (Chile) using FISH of tandemly repeated DNA sequences and RAPD analysis. Plant Sys Evol 269:1–14

    Article  CAS  Google Scholar 

  • Baeza M, Schrader O, Ruiz E, Negritto M (2008) Alstroemeria presliana Herb. (Alstroemeriaceae) in Chile from a cytogenetic perspective. Chil J Agric Res 68:328–333

    Article  Google Scholar 

  • Baeza C, Ruiz E, Negritto M (2010) Comparative karyotypic analysis in the Alstroemeria hookeri Lodd. (Alstroemeriaceae) complex sensu Bayer (1987). Gen Mol Biol 33:119–124

    Article  Google Scholar 

  • Baeza C, Finot V, Ruiz E (2015) Comparative karyotype analysis of populations in the Alstroemeria presliana Herbert (Alstroemeriaceae) complex in Chile. Gen Mol Biol 38:199–204

    Article  Google Scholar 

  • Bayer E (1987) Die Gattung Alstroemeria in Chile. Mitt Bot Staatssaml München 24:1–362

    Google Scholar 

  • Buitendijk J, Ramanna M (1996) Giemsa C-banded karyotypes of eight species of Alstroemeria L. and some of their hybrids. Ann Bot 78:449–457

    Article  Google Scholar 

  • Buitendijk J, Peters A, Jan-Quené R, Ramanna M (1998) Genome size variation and C-band polymorphism in Alstroemeria aurea, A. ligtu and A. magnifica (Alstroemeriaceae). Plant Syst Evol 212:87–106

    Article  Google Scholar 

  • Cajas D, Baeza C, Ruiz E, Negritto M (2009) Cytogenetic analysis in populations of Alstroemeria hookeri Lodd. subsp. hookeri (Alstroemeriaceae) from Bio-Bío Region, Chile. Gayana Bot 66:117–126

    Article  Google Scholar 

  • De Jeu M, Jacobsen E (1995) Early postfertilization ovule culture in Alstroemeria L. and barriers to interspecific hybridization. Euphytica 86:15–23

    Article  Google Scholar 

  • Finot VL, Baeza CM, Melín P, Novoa P, Campos J, Ruiz E, Fuentes G, Albornoz P, Carrasco P (2015) ¿Es Alstroemeria pulchra Sims var. maxima Phil. (Alstroemeriaceae) un miembro del complejo A. magnifica Herbert?: estudio colorimétrico de los tépalos externos e internos de la flor. Gayana Bot 72:101–113

    Article  Google Scholar 

  • González AV, Murúa M, Perez F (2014) Floral integration and pollinator diversity in the generalized plant-pollinator system of Alstroemeria ligtu (Alstroemeriaceae). Evol Ecol 29:63–75

    Article  Google Scholar 

  • Hutchinson M, Senaratna T, Tsujita J, Saxena P (1997) Somatic embryogenesis in liquid cultures of a tetraploid Alstroemeria. Pl Cell Tissue Org Cult 47:293–297

    Article  Google Scholar 

  • Levan A, Fredga K, Sandberg A (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220

    Article  Google Scholar 

  • Miyake I, Mobara S, Chiba K (1989) Breeding spotless Alstroemeria in Japan. Herbertia 45:40–44

    Google Scholar 

  • Muñoz M, Moreira A (2003) Alstroemerias de Chile. Diversidad, distribución y conservación. Taller La Era, Santiago

  • Negritto M, Baeza C, Ruiz E, Novoa P (2015) Alstroemeria marticorenae (Alstroemeriaceae), a new species from central Chile. Syst Bot 40:69–74

    Article  Google Scholar 

  • Peruzzi L, Eroglu H (2013) Karyotype asymmetry: again, how to measure and what to measure? Comp Cytogenetics 7:1–9

    Article  Google Scholar 

  • Ravenna P (1988) New or noteworthy species of Alstroemeria. Phytologia 64:281–288

    Article  Google Scholar 

  • Reeves A (2001) MicroMeasure: a new computer program for the collection and analysis of cytogenetic data. Genome 44:239–443

    Article  Google Scholar 

  • Zhou S, De Jeu M, Visser R, Kuipers A (2003) Characterization of distant Alstroemeria hybrids: applications of highly repetitive DNA sequences from A. ligtu ssp. ligtu. Ann Appl Biol 142:277–283

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by Fondecyt grant Nº 1130349. We thank Gabriele Kottirsch and Janina Baeza for technical assistance in field. We are grateful to the Minera Río Teno-Biobío Cementos S.A. for the authorization to collect material of Alstroemeria ligtu subsp. splendens.

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Authors and Affiliations

  1. Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile

    Carlos Baeza, Eduardo Ruiz & Pedro Carrasco

  2. Departamento de Producción Animal, Facultad de Agronomía, Universidad de Concepción, Chillán, Chile

    Víctor Finot

  3. Jardín Botánico de Viña del Mar, Corporación Nacional Forestal, Camino El Olivar 305, Viña Del Mar, Chile

    Patricio Novoa

  4. Herbarium, Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, 1315 Kinnear Road, Columbus, OH, 43212, USA

    Tod Stuessy

  5. Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile

    Alejandra González

Authors
  1. Carlos Baeza
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  2. Víctor Finot
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  3. Eduardo Ruiz
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  4. Pedro Carrasco
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  5. Patricio Novoa
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  6. Tod Stuessy
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  7. Alejandra González
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Corresponding author

Correspondence to Carlos Baeza.

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Baeza, C., Finot, V., Ruiz, E. et al. Comparative karyotypic analysis and cytotaxonomy in the Alstroemeria ligtu L. (Alstroemeriaceae) complex of Chile. Braz. J. Bot 39, 305–313 (2016). https://doi.org/10.1007/s40415-015-0220-4

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  • DOI: https://doi.org/10.1007/s40415-015-0220-4

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