Abstract
The paper studied germination characteristics, dehydration sensitivities, and antioxidative enzyme activities of seeds in three varieties of Camellia sinensis L. [C. sinensis var. sinensis cv. Fuding-dabaicha (Fd), C. sinensis var. assamica cv. Menghai-dayecha (Md), and C. sinensis var. assamica cv. Yunkang 10 (Y10)] during desiccation. The optimum germination temperature was 25 °C for Fd, 30 °C for Md, and 20 °C for Y10. Md, Y10, and Fd seeds were all sensitive to dehydration. GPs of three cultivars decreased dramatically after dehydration for 144 h, reaching values of 48, 37, and 57 % for Md, Y10, and Fd when moisture content declined to 17.5, 17.4, and 14.4 %, respectively. The antioxidative enzymes in Md, Y10, and Fd seeds were not as effective as orthodox seeds, none of them can scavenge ROS timely. Thus, lipid peroxidation accelerated. There were differences between seeds of the three cultivars: Fd seeds were smaller in shape than the other two; Fd seeds showed lower sensitivity to dehydration than the other two; and H2O2 contents in Fd seeds were obviously lower than in Md and Y10 seeds, indicating that antioxidative enzymes in Fd seeds were more effective than the other two.
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References
Aebi HE (1983) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analyses [M]. Verlag Chemie, Weinheim, pp 273–286
Anderson JW, Beardall J (1991) Molecular activities of plant cells. An introduction to plant biochemistry. Blackwell Scientific Publications, Oxford
Arrigoni O, De Gara L, Tommasi F, Liso R (1992) Changes in the ascorbate system during seed development of Viciafaba. Plant Physiol 99:235–238
Asada K (1992) Ascorbate peroxidase—a hydrogen peroxide scavenging enzyme in plants. Physiol Plant 85:235–241
Bailly C (2004) Active oxygen species and antioxidants in seed biology. Seed Sci Res 14:93–107
Bailly C, Benamar A, Corbineau F, Come D (1998) Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. Physiol Plant 104:646–652
Bailly C, Audigier A, Ladonne E, Wagner MH, Coste F, Corbineau F, Come D (2001) Changes in oligosaccharide content and antioxidant enzyme activities in developing bean seeds as related to acquisition of drying tolerance and seed quality. J Exp Bot 52:701–708
Berjak P, Vertucci CW, Pammenter NW (1993) Effects of developmental status and dehydration rate on characteristics of water and desiccation sensitivity in recalcitrant seeds of Camellia sinensis. Seed Sci Res 3:155–166
Bhattacharya A, Nagar PK, Ahuja PS (2002a) Seed development in Camellia sinensis (L.) O. Kuntze. Seed Sci Res 12:39–46
Bhattacharya A, Nagar PK, Ahuja PS (2002b) Seed development in Camellia sinensis (L.) O. Kuntze. Seed Sci Res 12:39–46
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding. Anal Biochem 72:248–254
Chandel KPS, Rekha C, Radhamani J, Malik SK (1995) Desiccation and freezing sensitivity in recalcitrant seeds of tea, cocoa and jackfruit. Ann Bot 76:443–450
Donahue JL, Okpodu CM, Cramer CL, Grabau EA, Alscher RG (1997) Responses of antioxidants to paraquat in pea leaves. Plant Physiol 113:249–257
Francini A, Galleschi L, Saviozzi F, Pinzino C, Izzo R, Sgherri C, Navari-Izzo F (2006) Enzymatic and non-enzymatic protective mechanisms in recalcitrant seeds of Araucaria bidwillii subject to desiccation. Plant Physiol Biochem 44:556–563
Halliwell B, Foyer CH (1978) Properties and physiological function of a glutathione reductase purified from spinach leaves by affinity chromatography. Planta 139:9–17
Hendry GAF, Thorpe PC, Merzlyak MN (1993) Stress indicators: lipid peroxidation. In: Hendry GAF, Grime JP (eds) Methods in Comparative Plant Ecology. Chapman & Hall, London, pp 154–156
Hong TD, Linington S, Ellis RH (1998) Compendium of information on seed storage behavior. R Bot Gard Kew Richmond Surrey 1:287–288
International Seed Testing Association (1999) International rules for seed testing. Seed Sci Technol 27(Supplement):47–50
Jiang M, Zhang J (2001) Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol 42:1265–1273
Kermode AR, Finch-Savage WE (2002) Desiccation sensitivity in orthodox and recalcitrant seeds in relation to development. In: Black M, Pritchard HW (eds) Desiccation and survival in plants. Drying without drying. CABI Publishing, New Haven, pp 149–184
Leprine O, Hendry GAF, Mckersie BD (1993) The mechanisms of desiccation tolerance in developing seeds. Seed Sci Res 3:231–246
Li CR, Sun WQ (1999) Desiccation sensitivity and activities of free radical-scavenging enzymes in recalcitrant Theobroma cacao seeds. Seed Sci Res 9:209–217
Nakayama A, Harada S (1962). Studies on the effect of temperature on the Growth of the tea plant. Part1: The effect of temperature on seed germination. Bull Tea Research Stn 33:3–80
Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Ann Rev Plant Mol Biol 49:249–279
Pammenter NW, Berjak P (1999) A review of recalcitrant seed physiology in relation to desiccation tolerance mechanisms. Seed Sci Res 9:13–37
Patterson BD, MacRae EA, Ferguson IB (1984) Estimation of hydrogen peroxide in plant extracts using titanium (IV). Anal Biochem 139:487–492
Pritchard HW, Daws ML, Fletcher BJ, Gamene CS, Msanga HP, Omond W (2004) Ecological correlates of seed desiccation tolerance in tropical African dryland trees. Am J Bot 91:863–870
Roberts EH (1973) Predicting the storage life of seeds. Seed Sci Technol 1:499–514
Stanislawa P, Ewelina R (2007) Ascorbate and glutathione metabolism during development and desiccation of orthodox and recalcitrant seeds of the genus Acer. Funct Plant Biol 34:601–613
Stanisława P, Ewelina R (2006) Antioxidative response of ascorbate-glutathione pathway enzymes and metabolites to desiccation of recalcitrant Acer saccharinum seeds. J Plant Physiol 163:1259–1266
Vertucci CW, Farrant JM (1995) Acquisition and loss of desiccation tolerance. In: Kigel J, Galili G (eds) Seed development and germination. Marcel Dekker Inc, New York, pp 237–271
Villiers TA (1978) Seed moisture and storage. Seed Sci Technol 6:993–996
Yan S, Gao R, Yin YP (2005) The relationship between moisture content of Ginkgo Biloba seeds and activity of reactive-oxygen-scavenging enzymes. Chin Agric Science Bull 21:207–210
Yu F, Du Y, Shen Y (2006) Physiological characteristics changes of Aesculus chinensis seeds during natural dehydration. J For Res 17:103–106
Acknowledgments
This study was funded by Special Grant for Basic Work of Science and Technology from the Ministry of Science and Technology, China (2012FY110300), the Supporting System Program of Strategic Biological Resources of the Chinese Academy of Sciences (CZBZX-1), and the Ministry of Science and Technology of China (2005DKA21006).
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Chen, Z.X., Lan, Q.Y., Zheng, L. et al. Effects of dehydration and temperature on seed viability and antioxidative enzymes activities on three kinds of cultivars of Camellia sinensis . Braz. J. Bot 38, 497–504 (2015). https://doi.org/10.1007/s40415-015-0167-5
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DOI: https://doi.org/10.1007/s40415-015-0167-5