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Effects of seed moisture content, temperature, and storage period on various physiological and biochemical parameters of Lallemantia iberica Fisch. & C.A.Mey

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Abstract

Alterations in physiological and biochemical parameters due to unfavorable storage conditions cause delayed germination, decreased germination rate, and even loss of viability of stored seeds. Therefore, these alterations constitute major problems for seed scientists, farmers, seed bank practitioners, and seed companies involved in seed production and storage. This research aimed to understand the mechanisms of seed ageing by the changing physiological and biochemical properties of Lallemantia iberica seeds. The seeds were stored with different moisture contents (5, 10, and 15%) at different temperatures (15 °C, 25 °C, 35 °C, and 45 °C) for 0, 30, 60, 90, and 120 days. Seed germinability was reduced from 100 to 0% by increasing seed moisture content, temperature, and storage period, while membrane integrity, as assayed by electrical conductivity (EC), K+/Na+ of the seed leachates, and malondialdehyde (MDA), was gradually compromised. Seed deterioration was characterized by exacerbation of membrane impairment, reduction of total soluble sugar, α-amylase and β-amylase activities as well as catalase (CAT) and ascorbate peroxidase (APX) activities. The results indicated a negative correlation of EC, K+/Na+, and MDA with germination indexes, vigor index, α-amylase and β-amylase activities, total soluble sugar, CAT, and APX levels. Generally, seed storage with 5% moisture content at 15 ℃ from 0 to 30 days had relatively high α-amylase and β-amylase activities, total soluble sugar, antioxidant enzyme activities, and low extents of membrane impairment. The results suggest that these storage conditions can be considered favorable for maintaining L. iberica seed quality; however, higher levels of the storage factors facilitate deterioration during storage.

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References

  • Adetunji AE, Adetunji TL, Varghese B, Pammenter NW (2021a) Oxidative stress, ageing and methods of seed invigoration: an overview and perspectives. Agron 11:2369

    Article  CAS  Google Scholar 

  • Adetunji AE, Varghese B, Pammenter N (2021b) Effects of exogenous application of five antioxidants on vigour, viability, oxidative metabolism and germination enzymes in aged cabbage and lettuce seeds. S Afr J Bot 137:85–97

    Article  CAS  Google Scholar 

  • Adetunji AE, Varghese B, Pammenter NW (2021c) Exogenous antioxidants enhance seedling growth and yield of artificially aged cabbage and lettuce seeds. Hortic 7:274

    Article  Google Scholar 

  • Adetunji AE, Varghese B, Pammenter NW (2020) Effects of inorganic salt solutions on vigour, viability, oxidative metabolism and germination enzymes in aged cabbage and lettuce seeds. Plants 9:1164

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Aghighi Shahverdi M, Omidi H, Mosanaiey H, Pessarakli M, Mousavi S, Ghasemzadeh M (2019) Effects of light and temperature treatments on germination and physiological traits of stevia seedling (Stevia rebuadiana Bertoni). J Plant Nutr 42:1125–1132

    Article  CAS  Google Scholar 

  • Bailly C, Benamar A, Corbineau F, Côme D (1998) Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. Physiol Plant 104:646–652

    Article  CAS  Google Scholar 

  • Bernfeld P (1955) Amylase Prot Meth Enzym 1:149–154

    CAS  Google Scholar 

  • Berry WL, Johnson C (1966) Determination of calcium and magnesium in plant material and culture solutions, using atomic-absorption spectroscopy. App Spectrosc 20:209–211

    Article  CAS  Google Scholar 

  • Boniecka J, Kotowicz K, Skrzypek E, Dziurka K, Rewers M, Jedrzejczyk I, Wilmowicz E, Berdychowska J, Dąbrowska GB (2019) Potential biochemical, genetic and molecular markers of deterioration advancement in seeds of oilseed rape (Brassica napus L.). Ind Crops Prod 130:478–490

    Article  CAS  Google Scholar 

  • Chen P, Cheng F, Wei L, Wang S, Zhang Z, Hang F, Li K, Xie C (2022) Effect of maillard reaction browning factors on color of membrane clarification non-centrifugal cane sugar during storage. Food Sci Technol. https://doi.org/10.1590/fst.43722

    Article  Google Scholar 

  • Chen X, Börner A, Xin X, Nagel M, He J, Li J, Li N, Lu X, Yin G (2021) Comparative proteomics at the critical node of vigor loss in wheat seeds differing in storability. Front Plant Sci e1883.

  • De Vitis M, Hay FR, Dickie JB, Trivedi C, Choi J, Fiegener R (2020) Seed storage: maintaining seed viability and vigor for restoration use. Restor Ecol 28:S249–S255

    Article  Google Scholar 

  • Demito A, Ziegler V, Goebel JTS, Konopatzki EA, Coelho SRM, Elias MC (2019) Effects of refrigeration on biochemical, digestibility, and technological parameters of Carioca beans L. during storage. Journal Food Biochem 43: e12900.

  • Ebone LA, Caverzan A, Chavarria G (2019) Physiologic alterations in orthodox seeds due to deterioration processes. Plant Physiol Biochem 145:34–42

    Article  CAS  PubMed  Google Scholar 

  • Ebone LA, Caverzan A, Silveira DC, Siqueira LdO, Lângaro NC, Chiomento JLT, Chavarria G (2020) Biochemical profile of the soybean seed embryonic axis and its changes during accelerated aging. Biolo 9:186

    Article  CAS  Google Scholar 

  • Farooq M, Ullah A, Lee D-J, Alghamdi SS, Siddique KH (2018) Desi chickpea genotypes tolerate drought stress better than kabuli types by modulating germination metabolism, trehalose accumulation, and carbon assimilation. Plant Physiol Biochem 126:47–54

    Article  CAS  PubMed  Google Scholar 

  • Fatokun K, Beckett RP, Varghese B (2022) A comparison of water imbibition and controlled deterioration in five orthodox species. Agron 12:1486

    Article  CAS  Google Scholar 

  • Garcia-Caparros P, De Filippis L, Gul A, Hasanuzzaman M, Ozturk M, Altay V, Lao MT (2021) Oxidative stress and antioxidant metabolism under adverse environmental conditions: a review. Bot Rev Bot Rev 87:421–466

    Article  Google Scholar 

  • Gasmi A, Peana M, Arshad M, Butnariu M, Menzel A, Bjørklund G (2021) Krebs cycle: activators, inhibitors and their roles in the modulation of carcinogenesis. Arch Toxicol 95:1161–1178

    Article  CAS  PubMed  Google Scholar 

  • Gerna D, Ballesteros D, Arc E, Stöggl W, Seal CE, Marami-Zonouz N, Na CS, Kranner I, Roach T (2022) Does oxygen affect ageing mechanisms of Pinus densiflora seeds? a matter of cytoplasmic physical state. J Exp Bot 73:2631–2649

    Article  CAS  PubMed  Google Scholar 

  • Gianella M, Doria E, Dondi D, Milanese C, Gallotti L, Börner A, Zannino L, Macovei A, Pagano A, Guzzon F (2022) Physiological and molecular aspects of seed longevity: exploring intra‐species variation in eight Pisum sativum L. accessions. Physiol Plant 13698.

  • Heath RL, Packer L (2018) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  Google Scholar 

  • International rules for seed testing [ISTA] (2013): International rules for seed testing: Weight determination. “Int. Seed Testing Assoc., Bassersdorf, Switzerland

  • Kang W, Suzuki M, Saito T, Miyado K (2021) Emerging role of tca cycle-related enzymes in human diseases. Int J Mol Sci 22:13057

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kijowska-Oberc J, Staszak AM, Ratajczak E (2021) Climate change affects seed aging? Initiation mechanism and consequences of loss of forest tree seed viability. Trees 35:1099–1108

    Article  CAS  Google Scholar 

  • Klupczyńska EA, Dietz K-J, Małecka A, Ratajczak E (2022) Mitochondrial peroxiredoxin-iif (prxiif) activity and function during seed aging. Antioxidants 11:1226

    Article  PubMed  PubMed Central  Google Scholar 

  • Kurek K, Plitta-Michalak B, Ratajczak E (2019) Reactive oxygen species as potential drivers of the seed aging process. Plants 8:174

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lee J-S, Hay FR (2020) Variation in seed metabolites between two indica rice accessions differing in seed longevity. Plants 9:1237

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li B-B, Zhang S-B, Lv Y-Y, Wei S, Hu Y-S (2022) Reactive oxygen species-induced protein carbonylation promotes deterioration of physiological activity of wheat seeds. PLoS ONE 17:e0263553

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li Z, Gao Y, Lin C, Pan R, Ma W, Zheng Y, Guan Y, Hu J (2018) Suppression of LOX activity enhanced seed vigour and longevity of tobacco (Nicotiana tabacum L.) seeds during storage. Conserv Physiol. 6: coy047.

  • Lin Y-x, Xu H-j, Yin G-k, Zhou Y-c, Lu X-x, Xin X (2022) Dynamic Changes in Membrane Lipid Metabolism and Antioxidant Defense During Soybean (Glycine max L. Merr.) Seed Aging Front Plant Sci. 13.

  • Małecka A, Ciszewska L, Staszak A, Ratajczak E (2021) Relationship between mitochondrial changes and seed aging as a limitation of viability for the storage of beech seed (Fagus sylvatica L.). PeerJ 9: e10569.

  • Mathias DJ, Kumar S, Rangarajan V (2019) An investigation on citrus peel as the lignocellulosic feedstock for optimal reducing sugar synthesis with an additional scope for the production of hydrolytic enzymes from the aqueous extract waste. Biocatal Agric Biotechnol 20:101259

    Article  Google Scholar 

  • Matilla AJ (2021) The orthodox dry seeds are alive: a clear example of desiccation tolerance. Plants 11:20

    Article  PubMed  PubMed Central  Google Scholar 

  • Murthy UN, Kumar PP, Sun WQ (2003) Mechanisms of seed ageing under different storage conditions for Vigna radiata (L.) Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. J Exp Bot 54:1057–1067

    Article  CAS  PubMed  Google Scholar 

  • Nagel M, Seal CE, Colville L, Rodenstein A, Un S, Richter J, Pritchard HW, Börner A, Kranner I (2019) Wheat seed ageing viewed through the cellular redox environment and changes in pH. Free Radic Res 53:641–654

    Article  CAS  PubMed  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Paravar A, Farahani SM, Rezazadeh A (2021) Lallemantia species response to drought stress and Arbuscular mycorrhizal fungi application. Ind Crops Prod 172:114002

    Article  CAS  Google Scholar 

  • Paravar A, Maleki Farahani S, Rezazadeh A (2018) Effect of drought stress during seed development on seed vigour, membrane peroxidation and antioxidant activity in different species of Balangu. J Crops Improv 20.

  • Paravar A, Maleki Farahani S, Rezazadeh A (2022) Lallemantia iberica and Lallemantia royleana: the effect of mycorrhizal fungal inoculation on growth and mycorrhizal dependency under sterile and non-sterile soils. Commun Soil Sci Plant Anal 53:880–891

    Article  CAS  Google Scholar 

  • Silva G, Sales J, Nascimento K, Rodrigues A, Camelo G, Borges E (2020) Biochemical and physiological changes in Dipteryx alata vog. seeds during germination and accelerated aging. S Afr J Bot 131:84–92

    Article  CAS  Google Scholar 

  • Sperling L, Gallagher P, McGuire S, March J, Templer N (2020) Informal seed traders: the backbone of seed business and African smallholder seed supply. Sustainability 12:7074

    Article  Google Scholar 

  • Stegner M, Wagner J, Roach T (2022) Antioxidant depletion during seed storage under ambient conditions. Seed Sci Res 1–7.

  • Steinfeld B, Scott J, Vilander G, Marx L, Quirk M, Lindberg J, Koerner K (2015) The role of lean process improvement in implementation of evidence-based practices in behavioral health care. J Behav Health Serv Res 42:504–518

    Article  PubMed  Google Scholar 

  • Stuffins C (1967) The determination of phosphate and calcium in feeding stuffs. Analyst 92:107–111

    Article  CAS  PubMed  Google Scholar 

  • Sudhakaran I (2020) Effect of seed ageing in biochemical and molecular changes in oilseeds: a review. Agric Rev 41:408–412

    Google Scholar 

  • Sun M, Sun S, Mao C, Zhang H, Ou C, Jia Z, Wang Y, Ma W, Li M, Jia S (2022) Dynamic Responses of Antioxidant and Glyoxalase Systems to Seed Aging Based on Full-Length Transcriptome in Oat (Avena sativa L.). Antioxidants 11: 395.

  • Suzuki Y, Ishiyama K, Yoon D-K, Takegahara-Tamakawa Y, Kondo E, Suganami M, Wada S, Miyake C, Makino A (2022) Suppression of chloroplast triose phosphate isomerase evokes inorganic phosphate-limited photosynthesis in rice. Plant Physiol 188:1550–1562

    Article  CAS  PubMed  Google Scholar 

  • Tian P-P, Lv Y-Y, Yuan W-J, Zhang S-B, Hu Y-S (2019) Effect of artificial aging on wheat quality deterioration during storage. J Stored Prod Res 80:50–56

    Article  Google Scholar 

  • Xia F, Cheng H, Chen L, Zhu H, Mao P, Wang M (2020) Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds. BMC Plant Biol 20:1–11

    Article  Google Scholar 

  • Xin X, Tian Q, Yin G, Chen X, Zhang J, Ng S, Lu X (2014) Reduced mitochondrial and ascorbate–glutathione activity after artificial ageing in soybean seed. J Plant Physiol 171:140–147

    Article  CAS  PubMed  Google Scholar 

  • Zhang K, Zhang Y, Sun J, Meng J, Tao J (2021) Deterioration of orthodox seeds during ageing: Influencing factors, physiological alterations and the role of reactive oxygen species. Plant Plant Physiol Biochem 158:475–485

    Article  CAS  PubMed  Google Scholar 

  • Ziegler V, Paraginski RT, Ferreira CD (2021) Grain storage systems and effects of moisture, temperature and time on grain quality-a review. J Stored Prod Res 91:101770

    Article  Google Scholar 

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Funded by Shahed University.

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Authors and Affiliations

  1. Department of Crop Production and Plant Breeding, College of Agriculture, Shahed University, Tehran, 18155-159, Iran

    Arezoo Paravar & Saeideh Maleki Farahani

  2. SAEON Ndlovu Node, Scientific Services, Kruger National Park, Phalaborwa, 1390, South Africa

    Ademola Emmanuel Adetunji

  3. Unit for Environmental Sciences and Management, Faculty of Natural and Agricultural Sciences, North-West University , Potchefstroom, 2520, South Africa

    Ademola Emmanuel Adetunji

  4. Department of Agronomy and Plant Breeding, University of Tehran, Karaj, 77871-31587, Iran

    Mostafa Oveisi & Ramin Piri

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  1. Arezoo Paravar
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Paravar, A., Maleki Farahani, S., Adetunji, A.E. et al. Effects of seed moisture content, temperature, and storage period on various physiological and biochemical parameters of Lallemantia iberica Fisch. & C.A.Mey. Acta Physiol Plant 45, 105 (2023). https://doi.org/10.1007/s11738-023-03581-0

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