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Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A

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Abstract

A double magnetic separation-assisted fluorescence method was developed to rapidly detect ochratoxin A(OTA). The OTA aptamer functionalized magnetic nanomaterial(Fe3O4-Aptamer) and complementary DNA conjugated nitrogen-doped graphene quantum dots(NGQDs-cDNA) were used in this assay. Aptamer could hybridize with cDNA, which induced the NGQDs-cDNA to bind onto Fe3O4-Aptamer, and resulted in the fluorescence quenching of NGQDs. After the addition of OTA, the NGQDs-cDNA could release into the solution, and resulted in the recovery of fluorescence signal of NGQDs consequently. By utilizing the magnetic separation, the unbonded NGQDs-cDNA and residual Fe3O4-Aptamer were removed, which significantly increased the fluorescence signal intensity. OTA could be detected in the linear range of 10 nmol/L to 2000 nmol/L, with a limit of detection as 0.66 nmol/L. The advantages of this method include simple operation, good selectivity and high sensitivity, and this method can be used for the rapid detection of ochratoxin A in wheat and corn.

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Authors and Affiliations

  1. College of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, P. R. China

    Chengke Wang, Rong Tan, Jiangyu Li & Zexiang Zhang

  2. Henan Key Laboratory of Biomolecular Recognition and Sensing, Shangqiu Normal University, Shangqiu, 476000, P. R. China

    Chengke Wang

Authors
  1. Chengke Wang
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  2. Rong Tan
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  3. Jiangyu Li
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  4. Zexiang Zhang
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Corresponding author

Correspondence to Chengke Wang.

Additional information

Supported by the National Natural Science Foundation of China(No.21305032), the China Postdoctoral Science Foundation (No.2014M551522), the Postdoctoral Science Foundation of Jiangsu Province, China(No.1402073B), the Henan Key Laboratory of Biomolecular Recognition and the Sensing, Shangqiu Normal University, China(No.HKLBRSK1803) and the Hong Kong Scholar Program, China (No.XJ2017008).

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Wang, C., Tan, R., Li, J. et al. Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A. Chem. Res. Chin. Univ. 35, 382–389 (2019). https://doi.org/10.1007/s40242-019-8322-3

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  • DOI: https://doi.org/10.1007/s40242-019-8322-3

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