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Bio-inspired cell membrane ingredient cholesterol-conjugated chitosan as a potential material for bone tissue repair

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Chemical Research in Chinese Universities Aims and scope

Abstract

We prepared a cholesterol-conjugated chitosan(CHCS) material and evaluated its potential application as a bone tissue repair material by in vitro cell experiments. Cell proliferation, differentiation and morphology on CHCS membrane surfaces with different graft degrees were assessed in mouse pre-osteoblasts MC3T3-E1 cells. The results indicate that CHCS materials could promote the proliferation of MC3T3-E1 cells at low graft degrees, but the CHCS material with high graft degree inhibits the proliferation of cells in contrast to the pure chitosan membrane. However, the alkaline phosphatase(ALP) activity of MC3T3-E1 cells on different CHCS membrane surface increased with increasing graft degrees of cholesterol. The area of cells stretched onto the surface of CHCS materials was larger than on the surface of CS materials, and more microfilaments and stress fibers in cells were observed on CHCS materials than on the pure chitosan material surface. After 7 d, the expression of related osteogenic marker genes, such as runt-related transcription factor 2(Runx2), osterix(OSX), osteocalcin(OCN), osteopontin(OPN), ALP and collagen I(COL-I) were all up-regulated in CHCS materials to different degrees compared to pure chitosan material, which indicated that the CHCS materials facilitated MC3T3-E1 cell differentiation and maturation. Characterizing CHCS materials is useful in designing and developing strategies for bone tissue engineering.

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Authors and Affiliations

  1. Department of Materials Science & Engineering, School of Science & Engineering, Jinan University, Guangzhou, 510632, P. R. China

    Zhenzhen Li, Jianhua Wen, Weijian Jia, Shan Ding, Xiaohui Xia & Changren Zhou

  2. Engineering Research Centre of Artificial Organs & Materials, Jinan University, Guangzhou, 510632, P. R. China

    Shan Ding & Changren Zhou

  3. Institute of Biomedicine & National Engineering Research Centre of Genetic Medicine, Jinan University, Guangzhou, 510632, P. R. China

    Yadong Huang

Authors
  1. Zhenzhen Li
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  2. Jianhua Wen
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  3. Weijian Jia
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  4. Shan Ding
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  5. Xiaohui Xia
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  6. Changren Zhou
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  7. Yadong Huang
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Corresponding authors

Correspondence to Shan Ding or Changren Zhou.

Additional information

Supported by the National Natural Science Foundation of China(Nos.31400824, 81171459) and the Science and Technology Project of Guangdong Province, China(No.2014A010105030).

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Li, Z., Wen, J., Jia, W. et al. Bio-inspired cell membrane ingredient cholesterol-conjugated chitosan as a potential material for bone tissue repair. Chem. Res. Chin. Univ. 32, 406–413 (2016). https://doi.org/10.1007/s40242-016-5510-2

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  • DOI: https://doi.org/10.1007/s40242-016-5510-2

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