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Synthesis and biocompatibility assessment of a cysteine-based nanocomposite for applications in bone tissue engineering

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Abstract

Purpose

To develop a robust ECM and enhance cellularmaterial interaction, we developed a cysteine-based nanocomposite combined with chitosan, nanohydroxyapatite and gelatin (cysteine-CnHAG) to effectuate essential factors.

Methods

The scaffolds were fabricated under same conditions using a freeze-drying technique and characterized with a scanning electron microscopy to study its internal architecture including pore distribution. They were further interacted with human bone marrow derived mesenchymal stem cells (hMSCs) to investigate its biocompatibility. In this, cell viability and cell adhesion was assessed using optical microscopy.

Results

From the results, the scaffolds display a non-toxic effect with hMSCs and have a pore size range of between 12-142 μm. The result of the optical microscopy showed adhesion of the cells on the scaffold matrix of both cysteine- CnHAG and CnHAG, which implies the microenvironment of the scaffold is largely suitable for the MSC development

Conclusions

In conclusion, we believe cysteine-CnHAG can be a novel nanocomposite for potential applications in the field of bone tissue engineering.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Pukyong National University, Busan, South Korea

    Baboucarr Lowe & Seung Yun Nam

  2. Interdisciplinary Program of Biomedical Mechanical and Electrical Engineering, Pukyong National University, Busan, South Korea

    Baboucarr Lowe & Seung Yun Nam

  3. Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, South Korea

    Seung Yun Nam

Authors
  1. Baboucarr Lowe
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  2. Seung Yun Nam
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Correspondence to Seung Yun Nam.

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Lowe, B., Nam, S.Y. Synthesis and biocompatibility assessment of a cysteine-based nanocomposite for applications in bone tissue engineering. Biomed. Eng. Lett. 6, 271–275 (2016). https://doi.org/10.1007/s13534-016-0239-x

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  • DOI: https://doi.org/10.1007/s13534-016-0239-x

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