Skip to main content
SpringerLink
Log in

A visualized fusion protein based on self-assembly hydrophobin HGFI

  • Published:
Chemical Research in Chinese Universities Aims and scope

Abstract

Hydrophobins are a type of small amphipathic proteins with a unique self-assembly property, which can be used to modify material surfaces and adsorb enzymes, antibodies and even cells. In this study, a fusion protein consisting of hydrophobin HGFI and green fluorescent protein(GFP) was successfully obtained from Pichia patoris (P. pastoris). Water contact angle(WCA) measurement proves that the wettability of the surfaces of different materials was changed. We further demonstrated the self-assembly ability of HGFI-GFP, which can be used to disperse the multi-walled carbon nanotubes(MWCNTs). Finally, the adsorption of HGFI-GFP onto the surface of the tissue engineering material poly(ε-caprolactone)(PCL) was evaluated by detecting the fluorescence of the fusion protein itself. The resalt demonstrates that both the basic self-assembly activity of the HGFI domain and the functional activity of the GFP domain were still remained.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wessels J. G., de Vries O. M., Asgeirsdottir S. A., Springer J., Journal of General Microbiology, 1991, 137, 2439

    Article  CAS  Google Scholar 

  2. Wessels J. G., Advances in Microbial Physiology, 1997, 38, 43

    Google Scholar 

  3. Bayry J., Aimanianda V., Guijarro J. I., Sunde M., Latge J. P., PLoS athogens, 2012, 8

    Google Scholar 

  4. Kershaw M. J., Thornton C. R., Wakley G. E., Talbot N. J., Molecular Microbiology, 2005, 56, 117

    Article  CAS  Google Scholar 

  5. Linder M. B., Current Opinion in Colloid & Interface Science, 2009, 14, 356

    Article  CAS  Google Scholar 

  6. Yu L., Zhang B., Szilvay G. R., Sun R., Janis J., Wang Z., Feng S., Xu H., Linder M. B., Qiao M., Microbiology-Sgm, 2008, 154, 1677

    Article  CAS  Google Scholar 

  7. Misra R., Li J., Cannon G. C., Morgan S. E., Biomacromolecules, 2006, 7, 1463

    Article  CAS  Google Scholar 

  8. De Stefano L., Rea I., Armenante A., Giardina P., Giocondo M., Rendina I., Langmuir, 2007, 23, 7921

    Article  Google Scholar 

  9. Zhao Z. X., Wang H. C., Qin X., Wang X. S., Qiao M. Q., Anzai J. I., Chen Q., Colloids and Surfaces B-Biointerfaces, 2009, 71, 104

    Article  Google Scholar 

  10. Corvis Y., Walcarius A., Rink R., Mrabet N. T., Rogalska E. Analytical Chemistry, 2005, 77, 1626

    Article  Google Scholar 

  11. Wang Z. F., Huang Y. J., Niu B. L., Li S., Wang D. D., Xu H. J., Qiao M. Q., Chem. J. Chinese Universities, 2010, 31(10), 2053

    Google Scholar 

  12. Hou S., Yang K., Qin M., Feng X. Z., Guan L., Yang Y., Wang C., Biosensors and Bioelectronics, 2008, 24, 912

    Article  CAS  Google Scholar 

  13. Niu B., Huang Y., Zhang S., Wang D., Xu H., Kong D., Qiao M., Protein Expression Purification, 2012, 83, 92

    Article  CAS  Google Scholar 

  14. Huang Y., Zhang S., Niu B., Wang D., Wang Z., Feng S., Xu H., Kong D., Qiao M., Colloids and Surfaces B-Biointerfaces, 2013, 101, 364

    Article  Google Scholar 

  15. Boeuf S., Throm T., Gutt B., Strunk T., Hoffmann M., Seebach E., Muehlberg L., Brocher J., Gotterbarm T., Wenzel W., Fischer R., Richter W., Acta Biomaterialia, 2012, 8, 1042

    Article  Google Scholar 

  16. Schagger H., Nature Protocols, 2006, 1, 17

    Article  Google Scholar 

  17. Swayne T. C., Boldogh I. R., Pon L. A., Cytoskeleton Methods and rotocols, 2009, 586, 179

    Google Scholar 

  18. Wang Z., Feng S., Huang Y., Li S., Xu H., Zhang X., Bai Y., Qiao M., Protein Expression and Purification, 2010, 72, 19

    Article  CAS  Google Scholar 

  19. Wang Z., Wang Y., Huang Y., Li S., Feng S., Xu H., Qiao M., Carbon, 2010, 48, 2896

    Google Scholar 

  20. Vizcaino-caston I., Wyre C., Overton T. W., Biotechnology Letters, 2012, 34, 178

    Article  Google Scholar 

  21. Wang H., Nakata E., Hamachi I., Chembiochem., 2009, 10, 2565

    Google Scholar 

  22. Pilbrough W., Munro T. P., Gray P., PLoS One, 2009, 4, e8432

    Article  Google Scholar 

  23. Jones J. J., Bridges A. M., Fosberry A. P., Gardner S., Lowes R. R., Newby R. R., James P. J., Hall R. M., Jenkins O., Journal of Biotechnology, 2004, 109, 204

    Article  Google Scholar 

  24. Zykwinska A., Guillemette T., Bouchara J. P., Cuenot S., Biochimica et Biophysica Acta(BBA)––Proteins and Proteomics, 2014, 1844, 1233

    Google Scholar 

  25. Zangi R., de Vocht M. L., Robillard G. T., Mark A., Biophysical Journal, 2002, 83, 113

    Article  Google Scholar 

  26. Wang X., Graveland-Bikker J. F., DeKruif C. G., Robillard G. T., Protein Science, 2004, 13, 815

    Google Scholar 

  27. Pedersen M. H., Borodina I., Moresco J. L., Svendsen W. E., Frisvad J. C., Sondergaard I., Applied Microbiology and Biotechnology, 2011, 90, 1926

    Article  Google Scholar 

  28. Askolin S., Linder M., Scholtmeijer K., Tenkanen M., Penttila M., de Vocht M. L., Wosten H. A. B., Biomacromolecules, 2006, 7, 1295

    Article  CAS  Google Scholar 

  29. Panjideh H., Coelho V., Dernedde J., Fuchs H., Keilholz U., Thiel E., Deckert P. M., Bioprocess Biosystems Engineering, 2008, 31, 563

    Article  Google Scholar 

  30. Qin M., Wang L. K., Feng X. Z., Yang Y. L., Wang R., Wang C., Yu L., Shao B., Qiao M. Q., Langmuir, 2007, 23, 4469

    Article  Google Scholar 

  31. Ding G. B., Liu H. Y., Wang Y., Lu Y. Y., Wu Y., Guo Y., Xu L., Chem. Res. Chinese Universities, 2013, 29(1), 105

    Article  CAS  Google Scholar 

  32. Winkler M., Raupp Y. S., Kohl L. A., Wagner H. E., Meier M. A. R., Macromolecules, 2014, 47, 2846

    Article  Google Scholar 

  33. Wang X., Wang H., Huang Y., Zhao Z., Qin X., Wang Y., Miao Z., Chen Q., Qiao M., Biosensors & Bioelectronics, 2010, 26, 1108

    Google Scholar 

  34. Palomo J. M., Penas M. M., Fernandez-Lorente G., Mateo C., Pisabarro A. G., Fernandez-Lafuente R., Ramirez L., Guisan J. M., Biomacromolecules, 2003, 4, 206

    Google Scholar 

  35. Chen Z., Zhang J., Guo Y., Zhang H., Cao J., Xu Q., Wang S., Wang B., Liu Z., Chem. Res. Chinese Universities, 2014, 30(4), 696

    Article  Google Scholar 

  36. Dai H., Xiao D. L., He H., Yuan D. H., Zhang C., Microchimica Acta, 2014, 182, 895

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, P. R. China

    Liqiang Zhao, Jinyuan Liu, Dongmin Song, Xiangxiang Wang, Feifei Tai, Haijin Xu & Mingqiang Qiao

Authors
  1. Liqiang Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jinyuan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dongmin Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiangxiang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Feifei Tai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Haijin Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mingqiang Qiao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mingqiang Qiao.

Additional information

Supported by the National Natural Science Foundation of China(No.31170066) and the Tianjin Key Research Program of Application Foundation and Advanced Technology, China(No.12JCZDJC22600).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhao, L., Liu, J., Song, D. et al. A visualized fusion protein based on self-assembly hydrophobin HGFI. Chem. Res. Chin. Univ. 31, 781–786 (2015). https://doi.org/10.1007/s40242-015-5135-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40242-015-5135-x

Keywords

Search

Navigation