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Metformin and alpha lipoic acid ameliorate hypothyroidism and its complications in adult male rats

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Abstract

Objective

The current study evaluates the effect of metformin (MET) and /or alpha lipoic acid (ALA) on hypothyroidism and its adverse effects on the cardiac, renal, and, hepatic functions in rats.

Materials and methods

Rats were divided into five groups: control, rat model of hypothyroidism induced by propylthiouracil (PTU), rat model of hypothyroidism treated with MET, rat model of hypothyroidism treated with ALA, and rat model of hypothyroidism treated with MET and ALA. At the end of the experiment, body weight gain was determined and the blood samples were collected from orbital plexus to measure the serum levels of thyroxine (T4), triiodothyronine (T3) and thyroid stimulating hormone (TSH) by ELISA, glucose level, the activities of lactate dehydrogenase (LDH), creatine kinase MB (CK-MB), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), and the levels of urea and creatinine spectrophotometrically.

Results

Rat model of hypothyroidism revealed a significant decrease in T4 (p < 0.001) and T3 (p < 0.001) and a significant increase in TSH (p < 0.005). This was accompanied by a significant decrease in the body weight gain (p < 0.025) and a significant increase in LDH (p < 0.001), CK-MB (p < 0.001) AST (p < 0.01), ALT (p < 0.016), ALP (p < 0.001), glucose (p < 0.001), urea (p < 0.001) and creatinine (p < 0.001). MET restored T4, T3 and TSH to control values. Treatment with ALA restored T3 and TSH levels. Treatment with Met and /or ALA reduced the levels of glucose, urea and creatinine and the activities of LDH, CK-MB, AST, ALT, and ALP to control-like values. Only ALA improved the reduced body weight gain induced by hypothyroidism.

Conclusion

The present findings indicate the ameliorative effects of MET and /or ALA on hypothyroidism and its adverse effects on cardiac, renal and hepatic functions.

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Authors and Affiliations

  1. Medical Physiology Department, Medical Research and Clinical Studies Institute, National Research Centre, El-Bohouth St, Giza, Egypt

    Noha M. Khoder, Hussein G. Sawie, Eman N. Hosny, Yasser A. Khadrawy & Mohga S. Abdulla

  2. Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt

    Hayat M. Sharada

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  1. Noha M. Khoder
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  2. Hussein G. Sawie
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Correspondence to Yasser A. Khadrawy.

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Khoder, N.M., Sawie, H.G., Sharada, H.M. et al. Metformin and alpha lipoic acid ameliorate hypothyroidism and its complications in adult male rats. J Diabetes Metab Disord 21, 1327–1337 (2022). https://doi.org/10.1007/s40200-022-01063-7

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