Advertisement

Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment

  • Saba Pirtarighat
  • Maryam Ghannadnia
  • Saeid Baghshahi
Open Access
Original Research
  • 121 Downloads

Abstract

Researchers use bionanotechnology techniques as eco-friendly and cost-effective routes to fabricate nanoparticles and nanomaterials. The present study confirms the ability of plant extract of Salvia spinosa grown under in vitro condition for the biosynthesis of silver nanoparticles (Ag NPs) for the first time. The surface plasmon resonance found at 450 nm confirmed the formation of Ag NPs. Moreover, FESEM images showed that nanoparticles had spherical morphology. Furthermore, XRD analysis confirmed the crystalline nature of the particles. FTIR analysis was carried out to identify possible biomolecules responsible in bioreduction of silver ions. Antimicrobial assay verified bactericidal activity of biosynthesized Ag NPs against Gram-positive and Gram-negative bacteria. According to the results, by growing the plants under controlled conditions, it is feasible to synthesize nanoparticles with desired properties.

Graphical abstract

Keywords

Bio-nanotechnology Salvia spinosa Silver nanoparticles Bactericidal activity 

Introduction

Nanotechnology is the synthesis of particles with at least one dimension in the range of 1–100 nm, resulting in high surface to volume ratios. As the particle size decreases, not only does the ratio of surface area to volume increase but also the physical, chemical and biological properties of the particles differ compared to their bulk counterparts [1, 2]. Noble-metal nanoparticles exhibit incredible physicochemical, optoelectronic and biochemical characteristics. They are being used for various purposes in industrial and pharmaceutical applications [3, 4]. Despite the existence of numerous metals in nature, only a few of them such as gold, silver, palladium and platinum are synthesized extensively in nanostructured form [5, 6]. Among the above-mentioned metals, silver nanoparticles have attracted much attention due to their unique characteristics for utilizing in various applications including pharmaceutics, agriculture, water detoxification, air filtration, textile industries and as a catalyst in oxidization reactions [7, 8, 9]. Furthermore, their predominant property is their high antibacterial activity against a broad range of bacteria without any toxicity to animal cells [10, 11, 12]. The establishment of resistance to antibiotics in bacteria especially multidrug resistance compelled scientists to explore novel compounds to halt multidrug-resistant microorganisms. Bactericidal activity of Ag NPs without toxicity to human cells can make them a proper substitution for antibiotics [13, 14]. Ag NPs are being employed for eliminating microorganisms in medical devices, implants and hospital masks [15, 16]. They are also extensively being used in hospitals either supplemented with antibiotics or alone for preventing infection [17]. Nanoparticles are traditionally synthesized broadly by physical and chemical procedures. Chemically prepared nanoparticles are not appropriate for medical usages due to hazardous chemicals binding on their surface. Furthermore, by-products produced in chemical routes are toxic for the environment. Physical routes for synthesis of NPs have some drawbacks, too. These methods require high energy and space, and are expensive [18, 19, 20]. Using biological systems such as microorganisms, plants, viruses and animal cell cultures is an alternative procedure for preparation of NPs [21, 22, 23]. In the most recent studies, researchers utilized Gelidium amansii [24], Enteromorpha compressa [25], Phanerochaete chrysosporium [26], Bacillus brevis [27] and Daucus carota [28] for the biosynthesis of Ag NPs and investigated their antimicrobial properties. Biosynthesis of NPs is eco-friendly, time affordable, cost effective. More importantly, the biosynthesized NPs are free of hazardous material on their surface. Also, they may be coated with bioorganic compounds that make them proper for medical applications. These give biosynthesis of NPs distinct advantages over conventional methods [29, 30]. For example, NPs are used as a new tool for targeting in cancer therapy and the most important difficulty is toxicity of NPs synthesized by previous methods. Scientists overcome this issue using biologically synthesized NPs coated with biomolecules that are more biocompatible [31, 32].

Although the exact mechanism of NPs biosynthesis by various plant extracts is ambiguous, it has been revealed that the biomolecules in plant extract such as protein, phenol and flavonoids play a significant role in the reduction of metals ions and capping the biosynthesized nanoparticles [33]. Culturing plants in controlled environmental systems presents an approach for the production of specific bioactive molecules in plants [34]. Since these plants are not exposed to environmental turbulence, the metabolic production of these plants is not dependent on environmental changes [35].

Medicinal plants have served as rich sources of pharmacologically active substances. Herbs have been used in a diverse array of purposes, including medicine, nutrition, flavoring, dying, repellents, fragrances, cosmetic, charms, smoking and industrial uses. Today, herbs are still found in 40% of prescription drugs [36]. The genus Salvia from Lamiaceae family includes almost 900 species cultured or grown as a weed all around the world, mainly in the areas of the Central and South America, and Mediterranean and South Africa [37]. The product of this plant is used for culinary as spices and pharmaceutical purposes. This genus offers an economical cultivate for small farmers due to its usage in folk remedies and ornamental purposes [38, 39]. The metabolite content of Salvia genus has been investigated thoroughly several times. The presence of flavonoids, caffeic acid esters such as rosmarinic acid, and phenolic diterpenes such as carnosic acid and carnosol has been detected in Salvia species extracts [34, 40].

The extract of various plants grown in farms is used permanently for the biosynthesis of nanoparticles, but whether the plants grown under in vitro condition have this ability or not is still under scrutiny. The main purposes of this work are considering the potential of the plant extract of S. spinosa grown in vitro in Murashige and Skoog (MS) medium for the biosynthesis of Ag NPs and investigation of their antibacterial activities against both Gram-positive and Gram-negative species of bacteria.

Materials and methods

Plant growth under in vitro condition

Seeds of S. spinosa were purchased from Qazvin farmers. They were surface sterilized by ethanol 70% for 1 min. After rinsing with sterile distilled water, sterilization was continued by soaking the seeds in sodium hypochlorite 2.5% for 5 min. This process is followed by three times rising with sterile distilled water for 10 min. Mineral salts, 3% sucrose and 4.5% agar were combined for Murashige and Skoog medium preparation and finally pH was adjusted at 5.8. After autoclaving, seeds were explanted in Petri dishes for germination. After germination, seedlings of the same age were subcultured into jars containing the same medium. These cultures were placed in the growth room at 25 ± 2 °C, in 16-/8-h period of day/night. After 2 months, plants were harvested and dried in oven at 37 °C for 48 h.

Preparation of plant extract

All chemicals were provided from Merck or Aldrich. The dried plants were powdered with a mortar and pestle. 10 ml of distilled water was poured to 0.2 g of plant powder. This combination was boiled for 5 min and then was cooled. The cooled solution was filtered with Whatman No. 1 filter paper.

Biosynthesis of Silver nanoparticles

Aqueous solution of silver nitrate (1 mM) was prepared and mixed with fresh plant extract of S. spinosa at a ratio of 9:1. This solution was placed on a shaker with constant rotation in the room temperature at 27 ± 2°C for 6 h. All stages of the experiment were implemented in three replicates.

Characterization of the silver nanoparticles

Conventionally, characterization of particles is implemented by UV–Vis spectroscopy. An UV–Vis spectrophotometer (UVD 3200) was employed for verifying the biosynthesized Ag NPs. An Equinox 3000 diffractometer was utilized for XRD studies of Ag NPs. Furthermore, biosynthesized Ag NPs were observed by field-emission scanning electron microscopy (FESEM, HITACHI, S-4160). Particle size distribution of biosynthesized Ag NPs was obtained using Dynamic Light Scattering Malvern-Zetasizer (Nno-z 590). This study was undertaken to know the functional groups existed in plant extract responsible in silver ion reduction, so the pellets of plant extract and biosynthesized Ag NPs with potassium bromide (1:100) were used for FTIR spectrum analysis of plant extract and biosynthesized Ag NPs by a Bruker Tensor 27 spectrophotometer.

Antibacterial activity of biosynthesized Ag NPs

The antibacterial activity of the biosynthesized Ag NPs against Gram-positive and -negative bacteria species was done by disk diffusion method. Experimented bacteria were Bacillus subtilis (accession number: M59 KP406766), Bacillus vallismortis (accession number: M92 KP406765) and Escherichia coli (PTCC: 1276). Bacterial strains were spread on the Petri dishes which contained autoclaved Luria–Bertani (LB) medium containing agar. Then, the disks (6 mm diameter) soaked in distilled water as a control, plant extract and biosynthesized Ag NPs were separately placed on Petri dishes containing LB media. Petri dishes were incubated at 37 °C. Inhibition zone of each disk was measured by ruler after 18 h.

Results and discussion

Color change of solution

In this study, we cultured plants in vitro under controlled conditions and used these plants for considering their potential for reduction of Ag ions. The fresh extract of S. spinosa was yellow in color, but after addition of AgNO3 solution and stirring at room temperature, gradually the solution color changed into red (Fig. 1). In other words, by passing the incubation time, the color intensity increased, which confirmed Ag ion reduction and the formation of Ag NPs [41]. Silver nanoparticle surface plasmon excitation causes color change in the solution [42], which is the primary and notable evidence for the formation of Ag NPs [43].
Fig. 1

Color changes in the plant extract after adding AgNO3 solution. a S. spinosa grown in vitro b 5 min and c 6 h after reaction

UV–Vis analysis

UV–Vis absorption spectrum of Ag NPs is shown in Fig. 2. Broad bell-shaped spectrum curve was obtained from UV–Vis analysis. Various metabolites from plant extract introduced to solution make the plasmon band broad because they may be read in this spectrophotometric range, too. Surface plasmon resonance (SPR) of silver occurs at 450 nm. This peak increased with time up to 360 min. According to Mie theory, spherical nanoparticles show only a single SPR band. The number of peaks increases by increasing diversity of particles shapes [43, 44]. Then, it can be concluded that biosynthesized Ag NPs are unanimously spherical in nature.
Fig. 2

UV–Vis absorption spectra of silver nanoparticles biosynthesized by S. spinosa extract during reaction

X-ray diffraction analysis

XRD pattern revealed distinct peaks at 2θ values, which can be attributed to 111, 200, 220 and 311 crystalline planes of Ag NPs (Fig. 3). These peaks are associated with the face-centered cubic lattice [45]. Other peaks at 2θ values in Ag NPs pattern can be ascribed to the residues of the organic content of the plant extract. These peaks reveal the crystallization of some plant metabolite moieties on the surface of the Ag NPs, which is in agreement with Shanmuganathan et al.’s results [28]. This is an acceptable evidence to confirm the involvement of the plant extract compositions in the Ag NP formation. This result is in accordance with XRD analysis of Oves et al. [46]. Although the exact mechanism of biosynthesis procedure by plant extract is not clear, the role of the secondary metabolites in biosynthesis process is undeniable. The presence of carnosic acid and flavonoids, which contain carboxylate group, have been detected in Salvia species extracts [34]. Interactions between these metabolites and silver ions cause the bioreduction of silver nitrate and synthesis of Ag NPs. Negatively charged groups presented in the plant extract such as carboxylate (COO) and polar groups such as OH and CO have a high tendency to attach on the surface of the Ag+. So, these groups contribute in both reduction and stabilization of Ag ions [47]. It can be concluded that plant metabolites containing OH, CO and especially COO such as carnosic acid, flavonoids and proteins have a pivotal role in the possible mechanism for bioreduction of silver ions.
Fig. 3

X ray diffraction profile from dried silver nanoparticles biosynthesized by S. spinosa extract

FESEM and DLS analysis

The morphology of the Ag NPs was analyzed through FESEM images (Fig. 4a, b). The majority of particles were spherical in shape and there were a few oval Ag NPs as well. Biosynthesized Ag NPs had been spread thoroughly in the solution. The size of some selected biosynthesized nanoparticles was 19–125 nm according to FESEM images. The average diameter of total particles was measured by dynamic light scattering (DLS) analysis. The majority of the Ag NPs were 5.13 nm in diameter (Fig. 4c). The biomolecule coating of the biosynthesized Ag NPs is shown in the FESEM images. This layer confirms plant extract metabolites’ role in the synthesis and stabilizing of the biosynthesized Ag NPs. These results are in agreement with Oves et al.’s [46] findings.
Fig. 4

a) FESEM images scale in a 500 nm and b 1 µm. Nanoparticles coated with organic materials of plant extract have been indicated with arrows in two pictures c particle diameter analysis from Ag NPs biosynthesized by S. spinosa extract

Fourier infrared spectroscopy analysis

FTIR spectra of the biosynthesized silver nanoparticles and the aqueous plant extract are shown in Fig. 5. Responsible metabolites for reduction of Ag ions were detected using FTIR spectrum. Instinct absorption bands at 3423.15 and 3420.77 cm−1 appear in the presence of phenols and alcohols with free OH group [48]. This band is superimposed by NH stretching peak [49]. The bands at 2928.45 and 2928.44 cm−1 represent the presence of alkanes in lipids [48]. The region of 2359.03 cm−1 indicates the presence of symmetric stretching of COO just in the biosynthesized solution [47]. The bands at 2240.59 and 2241.03 cm−1 are assigned to the C≡C group. The bands at 2138.93 and 2138.90 cm−1 reveal the presence of alkynes, N–C and N=C groups in R–N=C=S structure. The absorption bands at 1630.32 and 1631.43 cm−1 are corresponded to the C=C in aromatic compounds and amide I (NH out of plane) [50, 51]. The bands at 1518.75 and 1518.92 cm−1 are due to the amide II in proteins [51]. The bands at 1406.56 and 1407.48 cm−1 indicate S=O (sulfate ester) group. The bands at 1123 and 1150.67 cm−1 confirmed the presence of carboxylic acid group. The bands at 821.69 and _ cm−1, and also 674.63 and 675.51 cm−1 represent the presence of =CH in aromatic compounds in the plant extract and the biosynthesized Ag NPs. The bands at 603.71 and 602.71 cm−1 correspond to the C–Cl (alkyl halides). First number belongs to the plant extract and second to the Ag NPs. Free carboxylate groups presented in proteins can bind to the metal nanoparticles and make them stable [47]. Only minor changes in the position of absorption bands between FTIR spectrum of the plant extract and biosynthesized Ag NPs were seen. The involvement of plant extract compounds in biosynthesizing of nanoparticles was confirmed with shifting of peaks. Thus, plant extract compounds including OH and CO groups have a vital role in reducing and stabilization of NPs [52].
Fig. 5

FTIR spectra of biosynthesized Ag NPs by S. spinosa extract and S. spinosa extract

Antibacterial activity of the Ag NPs

Antibacterial activity of the biosynthesized Ag NPs is shown in Fig. 6. Distilled water was utilized as a control. The measured diameter for the restriction area for the growth of bacteria in B. subtilis, B. vallismortis and E. coli were 15, 16 and 12, respectively. This result indicates that Gram-positive bacteria are more sensitive than Gram-negative bacteria. The bactericidal activity of Ag NPs against broad range of bacteria has been proven in several studies. This potential of Ag NPs confirms multifaceted strategy of Ag NPs in the bacteria exposure [53]. The mechanism of bactericidal activity of Ag NPs is most likely due to the attachment of the Ag NPs to the cell wall and the generation of free radicals. Moreover, the presence of Ag NPs in the cell membrane of bacteria has been proven in earlier studies [46]. Ag NPs disturb the permeability of the membrane by penetrating to the cell membrane and causing intracellular ATP leakage and cell death [47, 54]. Silver ions’ release from Ag NPs acting as reservoir causes antibacterial activity of Ag NPs [47]. It appears that the positively charged ions such as Ag+ in this experiment have a high tendency to act with phosphorus and sulfur presented in biomolecules such as DNA and RNA. Such an attachment results in the disruption of DNA and RNA functions [54, 55].
Fig. 6

Bactericidal activity of (1) S. spinosa extract, (2) distilled water and (3) biosynthesized Ag NPs from S. spinosa extract, against a E. coli, b B. subtilis and c B. vallismortis

Conclusion

Biosynthesis of Ag NPs using plant extract of S. spinosa grown in vitro under controlled condition was carried out for the first time in this study. This study confirmed S. spinosa’s (cultured under controlled condition) capability for the biosynthesis of Ag NPs. The characteristics of the biosynthesized Ag NPs were measured by different equipments. Moreover, bactericidal activity assessment of the biosynthesized Ag NPs showed their inhibitory function against both Gram-positive and Gram-negative bacteria. In this study, possible functional groups and effective compounds responsible in reduction of silver ions were assigned. The speculated mechanism in this work elucidates the involvement of carboxylic acid functional group presented in carnosic acid and flavonoids in the bioreduction process and the stabilization of biosynthesized Ag NPs.

Notes

Acknowledgements

Authors are thankful for Imam Khomeini International University authorities for supporting this work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Dos Santos, C.A., Seckler, M.M., Ingle, A.P., Gupta, I., Galdiero, S., Galdiero, M., Gade, A., Rai, M.: Silver nanoparticles: therapeutical uses, toxicity, and safety issues. J. Pharm. Sci. 103, 1931–1944 (2014)CrossRefGoogle Scholar
  2. 2.
    Narayanan, K.B., Sakthivel, N.: Biological synthesis of metal nanoparticles by microbes. Adv. Colloid Interface Sci. 156, 1–13 (2010)CrossRefGoogle Scholar
  3. 3.
    Linic, S., Aslam, U., Boerigter, C., Morabito, M.: Photochemical transformations on plasmonic metal nanoparticles. Nat. Mater. 14, 567–576 (2015)CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Thakkar, K.N., Mhatre, S.S., Parikh, R.Y.: Biological synthesis of metallic nanoparticles. Nanomed. Nanotechnol. Biol. Med. 6, 257–262 (2010)CrossRefGoogle Scholar
  5. 5.
    Yoon, W.-J., Jung, K.-Y., Liu, J., Duraisamy, T., Revur, R., Teixeira, F.L., Sengupta, S., Berger, P.R.: Plasmon-enhanced optical absorption and photocurrent in organic bulk heterojunction photovoltaic devices using self-assembled layer of silver nanoparticles. Sol. Energy Mater. Sol. Cells 94, 128–132 (2010)CrossRefGoogle Scholar
  6. 6.
    Yang, Y., Jin, P., Zhang, X., Ravichandran, N., Ying, H., Yu, C., Ying, H., Xu, Y., Yin, J., Wang, K.: New epigallocatechin gallate (EGCG) nanocomplexes co-assembled with 3-mercapto-1-hexanol and β-lactoglobulin for improvement of antitumor activity. J. Biomed. Nanotechnol. 13, 805–814 (2017)CrossRefGoogle Scholar
  7. 7.
    Sathishkumar, M., Sneha, K., Won, S., Cho, C.-W., Kim, S., Yun, Y.-S.: Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf. B 73, 332–338 (2009)CrossRefGoogle Scholar
  8. 8.
    Gan, L., Zhang, S., Zhang, Y., He, S., Tian, Y.: Biosynthesis, characterization and antimicrobial activity of silver nanoparticles by a halotolerant Bacillus endophyticus SCU-L. Prep. Biochem. Biotechnol. A (2018).  https://doi.org/10.1080/10826068.2018.1476880 CrossRefGoogle Scholar
  9. 9.
    Arvizo, R.R., Bhattacharyya, S., Kudgus, R.A., Giri, K., Bhattacharya, R., Mukherjee, P.: Intrinsic therapeutic applications of noble metal nanoparticles: past, present and future. Chem. Soc. Rev. 41, 2943–2970 (2012)CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Elechiguerra, J.L., Burt, J.L., Morones, J.R., Camacho-Bragado, A., Gao, X., Lara, H.H., Yacaman, M.J.: Interaction of silver nanoparticles with HIV-1. J. Nanobiotechnol. 3, 6 (2005).  https://doi.org/10.1186/1477-3155-3-6 CrossRefGoogle Scholar
  11. 11.
    Yeo, S.Y., Lee, H.J., Jeong, S.H.: Preparation of nanocomposite fibers for permanent antibacterial effect. J. Mater. Sci. 38, 2143–2147 (2003)CrossRefGoogle Scholar
  12. 12.
    Elshawy, O.E., Helmy, E.A., Rashed, L.A.: Preparation, characterization and in vitro evaluation of the antitumor activity of the biologically synthesized silver nanoparticles. Adv. Nanopart. 5, 149–166 (2016)CrossRefGoogle Scholar
  13. 13.
    Leid, J.G., Ditto, A.J., Knapp, A., Shah, P.N., Wright, B.D., Blust, R., Christensen, L., Clemons, C., Wilber, J., Young, G.W.: In vitro antimicrobial studies of silver carbene complexes: activity of free and nanoparticle carbene formulations against clinical isolates of pathogenic bacteria. J. Antimicrob. Chemother. 67, 138–148 (2011)CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Modi, S.R., Collins, J.J., Relman, D.A.: Antibiotics and the gut microbiota. J. Clin. Investig. 124, 4212–4218 (2014)CrossRefPubMedGoogle Scholar
  15. 15.
    Ansari, M., Khan, H., Khan, A., Cameotra, S., Alzohairy, M.: Anti-biofilm efficacy of silver nanoparticles against MRSA and MRSE isolated from wounds in a tertiary care hospital. Indian J. Med. Microbiol. 33, 101–109 (2015)CrossRefPubMedGoogle Scholar
  16. 16.
    Bosetti, M., Masse, A., Tobin, E., Cannas, M.: Silver coated materials for external fixation devices: in vitro biocompatibility and genotoxicity. Biomaterials 23, 887–892 (2002)CrossRefPubMedGoogle Scholar
  17. 17.
    Shahverdi, A.R., Fakhimi, A., Shahverdi, H.R., Minaian, S.: Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomed. NBM 3, 168–171 (2007)CrossRefGoogle Scholar
  18. 18.
    Gade, A., Bonde, P., Ingle, A., Marcato, P., Duran, N., Rai, M.: Exploitation of Aspergillus niger for synthesis of silver nanoparticles. J. Biobased Mater. Bioenergy 2, 243–247 (2008)CrossRefGoogle Scholar
  19. 19.
    Ouda, S.M.: Antifungal activity of silver and copper nanoparticles on two plant pathogens, Alternaria alternata and Botrytis cinerea. Res. J. Microbiol. 9, 34–42 (2014)CrossRefGoogle Scholar
  20. 20.
    Wei, X., Luo, M., Li, W., Yang, L., Liang, X., Xu, L., Kong, P., Liu, H.: Synthesis of silver nanoparticles by solar irradiation of cell-free Bacillus amyloliquefaciens extracts and AgNO3. Bioresour. Technol. 103, 273–278 (2012)CrossRefPubMedGoogle Scholar
  21. 21.
    Naik, R.R., Stringer, S.J., Agarwal, G., Jones, S.E., Stone, M.O.: Biomimetic synthesis and patterning of silver nanoparticles. Nat. Mater. 1, 169–172 (2002)CrossRefPubMedGoogle Scholar
  22. 22.
    Fayaz, A.M., Balaji, K., Girilal, M., Yadav, R., Kalaichelvan, P.T., Venketesan, R.: Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomed. NBM 6, 103–109 (2010)CrossRefGoogle Scholar
  23. 23.
    Singhal, G., Bhavesh, R., Kasariya, K., Sharma, A.R., Singh, R.P.: Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J. Nanopart. Res. 13, 2981–2988 (2011)CrossRefGoogle Scholar
  24. 24.
    Pugazhendhi, A., Prabakar, D., Jacob, J.M., Karuppusamy, I., Saratale, R.G.: Synthesis and characterization of silver nanoparticles using Gelidium amansii and its antimicrobial property against various pathogenic bacteria. Microb. Pathog. 114, 41–45 (2018)CrossRefPubMedGoogle Scholar
  25. 25.
    Ramkumar, V.S., Pugazhendhi, A., Gopalakrishnan, K., Sivagurunathan, P., Saratale, G.D., Dung, T.N.B., Kannapiran, E.: Biofabrication and characterization of silver nanoparticles using aqueous extract of seaweed Enteromorpha compressa and its biomedical properties. Biotechnol. Rep. 14, 1–7 (2017)CrossRefGoogle Scholar
  26. 26.
    Saravanan, M., Arokiyaraj, S., Lakshmi, T., Pugazhendhi, A.: Synthesis of silver nanoparticles from Phanerochaete chrysosporium (MTCC-787) and their antibacterial activity against human pathogenic bacteria. Microb. Pathog. 117, 68–72 (2018)CrossRefPubMedGoogle Scholar
  27. 27.
    Saravanan, M., Barik, S.K., MubarakAli, D., Prakash, P., Pugazhendhi, A.: Synthesis of silver nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against pathogenic bacteria. Microb. Pathog. 116, 221–226 (2018)CrossRefPubMedGoogle Scholar
  28. 28.
    Shanmuganathan, R., MubarakAli, D., Prabakar, D., Muthukumar, H., Thajuddin, N., Kumar, S.S., Pugazhendhi, A.: An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach. Environ. Sci. Pollut. Res. 25, 10362–10370 (2018)CrossRefGoogle Scholar
  29. 29.
    Mukherjee, P., Roy, M., Mandal, B., Dey, G., Mukherjee, P., Ghatak, J., Tyagi, A., Kale, S.: Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T. asperellum. Nanotechnology 19, 75103–75110 (2008)CrossRefGoogle Scholar
  30. 30.
    Bar, H., Bhui, D.K., Sahoo, G.P., Sarkar, P., De, S.P., Misra, A.: Green synthesis of silver nanoparticles using latex of Jatropha curcas. Colloids Surf. A 339, 134–139 (2009)CrossRefGoogle Scholar
  31. 31.
    Pugazhendhi, A., Edison, T.N.J.I., Karuppusamy, I., Kathirvel, B.: Inorganic nanoparticles: a potential cancer therapy for human welfare. Int. J. Pharm. 539, 104–111 (2018)CrossRefGoogle Scholar
  32. 32.
    Khandekar, S.V., Kulkarni, M., Devarajan, P.V.: Polyaspartic acid functionalized gold nanoparticles for tumor targeted doxorubicin delivery. J. Biomed. Nanotechnol. 10, 143–153 (2014)CrossRefGoogle Scholar
  33. 33.
    Krishnaraj, C., Jagan, E., Rajasekar, S., Selvakumar, P., Kalaichelvan, P., Mohan, N.: Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf. B 76, 50–56 (2010)CrossRefGoogle Scholar
  34. 34.
    Luis, J.G.: Chemistry, biogenesis, and chemotaxonomy of the diterpenoids of Salvia. In: Harborne, J.B., Tomas-Barberan, F.A. (eds.) Ecological Chemistry and Biochemistry of Plant Terpenoids, pp. 63–82. Clarendon Press, Oxford (1991)Google Scholar
  35. 35.
    Grzegorczyk, I., Matkowski, A., Wysokińska, H.: Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chem. 104, 536–541 (2007)CrossRefGoogle Scholar
  36. 36.
    Newman, D.J., Cragg, G.M.: Natural products as sources of new drugs over the last 25 years. J. Nat. Prod. 70, 461–477 (2007)CrossRefGoogle Scholar
  37. 37.
    Delamare, A.P.L., Moschen-Pistorello, I.T., Artico, L., Atti-Serafini, L., Echeverrigaray, S.: Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chem. 100, 603–608 (2007)CrossRefGoogle Scholar
  38. 38.
    Maksimović, M., Vidic, D., Miloš, M., Šolić, M.E., Abadžić, S., Siljak-Yakovlev, S.: Effect of the environmental conditions on essential oil profile in two Dinaric Salvia species: S. brachyodon Vandas and S. officinalis L. Biochem. Syst. Ecol. 35, 473–478 (2007)CrossRefGoogle Scholar
  39. 39.
    Taarit, M.B., Msaada, K., Hosni, K., Hammami, M., Kchouk, M.E., Marzouk, B.: Plant growth, essential oil yield and composition of sage (Salvia officinalis L.) fruits cultivated under salt stress conditions. Ind. Crops Prod. 30, 333–337 (2009)CrossRefGoogle Scholar
  40. 40.
    Grzegorczyk, I., Bilichowski, I., Mikiciuk-Olasik, E., Wysokinska, H.: In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds. Acta Soc. Bot. Pol. 74, 17–21 (2005)CrossRefGoogle Scholar
  41. 41.
    Chandran, S.P., Chaudhary, M., Pasricha, R., Ahmad, A., Sastry, M.: Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol. Prog. 22, 577–583 (2006)CrossRefPubMedGoogle Scholar
  42. 42.
    Kumar, P., Selvi, S.S., Govindaraju, M.: Seaweed-mediated biosynthesis of silver nanoparticles using Gracilaria corticata for its antifungal activity against Candida spp. Appl. Nanosci. 3, 495–500 (2013)CrossRefGoogle Scholar
  43. 43.
    Banerjee, P., Satapathy, M., Mukhopahayay, A., Das, P.: Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour. Bioprocess. 1, 1–10 (2014)CrossRefGoogle Scholar
  44. 44.
    Raut Rajesh, W., Lakkakula Jaya, R., Kolekar Niranjan, S., Mendhulkar Vijay, D., Kashid Sahebrao, B.: Phytosynthesis of silver nanoparticle using Gliricidia sepium (Jacq.). Curr. Nanosci. 5, 117–122 (2009)CrossRefGoogle Scholar
  45. 45.
    de Jesús Ruíz-Baltazar, Á., Reyes-López, S.Y., Larrañaga, D., Estévez, M., Pérez, R.: Green synthesis of silver nanoparticles using a Melissa officinalis leaf extract with antibacterial properties. Results Phys. 7, 2639–2643 (2017)CrossRefGoogle Scholar
  46. 46.
    Oves, M., Aslam, M., Rauf, M.A., Qayyum, S., Qari, H.A., Khan, M.S., Alam, M.Z., Tabrez, S., Pugazhendhi, A., Ismail, I.M.: Antimicrobial and anticancer activities of silver nanoparticles synthesized from the root hair extract of Phoenix dactylifera. Mater. Sci. Eng. C 89, 429–443 (2018)CrossRefGoogle Scholar
  47. 47.
    Ajitha, B., Reddy, Y.A.K., Reddy, P.S.: Biogenic nano-scale silver particles by Tephrosia purpurea leaf extract and their inborn antimicrobial activity. Spectrochimica Acta, Part A 121, 164–172 (2014)CrossRefGoogle Scholar
  48. 48.
    Vanaja, M., Gnanajobitha, G., Paulkumar, K., Rajeshkumar, S., Malarkodi, C., Annadurai, G.: Phytosynthesis of silver nanoparticles by Cissus quadrangularis: influence of physicochemical factors. J. Nanostruct. Chem. 3, 1–8 (2013)CrossRefGoogle Scholar
  49. 49.
    Suresh, S., Karthikeyan, S., Jayamoorthy, K.: FTIR and multivariate analysis to study the effect of bulk and nano copper oxide on peanut plant leaves. J. Sci. Adv. Mater. Devices. 1, 343–350 (2016)CrossRefGoogle Scholar
  50. 50.
    Raghunandan, D., Bedre, M.D., Basavaraja, S., Sawle, B., Manjunath, S., Venkataraman, A.: Rapid biosynthesis of irregular shaped gold nanoparticles from macerated aqueous extracellular dried clove buds (Syzygium aromaticum) solution. Colloids Surf. B 79, 235–240 (2010)CrossRefGoogle Scholar
  51. 51.
    Stehfest, K., Toepel, J., Wilhelm, C.: The application of micro-FTIR spectroscopy to analyze nutrient stress-related changes in biomass composition of phytoplankton algae. Plant Physiol. Biochem. 43, 717–726 (2005)CrossRefPubMedGoogle Scholar
  52. 52.
    Sre, P.R., Reka, M., Poovazhagi, R., Kumar, M.A., Murugesan, K.: Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam. Spectrochim. Acta, Part A 135, 1137–1144 (2015)CrossRefGoogle Scholar
  53. 53.
    Franci, G., Falanga, A., Galdiero, S., Palomba, L., Rai, M., Morelli, G., Galdiero, M.: Silver nanoparticles as potential antibacterial agents. Molecules 20, 8856–8874 (2015)CrossRefPubMedGoogle Scholar
  54. 54.
    Hajipour, M.J., Fromm, K.M., Ashkarran, A.A., de Aberasturi, D.J., de Larramendi, I.R., Rojo, T., Serpooshan, V., Parak, W.J., Mahmoudi, M.: Antibacterial properties of nanoparticles. Trends Biotechnol. 30, 499–511 (2012)CrossRefPubMedGoogle Scholar
  55. 55.
    Umashankari, J., Inbakandan, D., Ajithkumar, T.T., Balasubramanian, T.: Mangrove plant, Rhizophora mucronata (Lamk, 1804) mediated one pot green synthesis of silver nanoparticles and its antibacterial activity against aquatic pathogens. Aquat. Biosyst. 8, 1–8 (2012)CrossRefGoogle Scholar

Copyright information

© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Saba Pirtarighat
    • 1
  • Maryam Ghannadnia
    • 2
  • Saeid Baghshahi
    • 3
  1. 1.Department of BiotechnologyImam Khomeini International University (IKIU)QazvinIslamic Republic of Iran
  2. 2.Department of Horticultural ScienceImam Khomeini International University (IKIU)QazvinIslamic Republic of Iran
  3. 3.Department of Materials Science and EngineeringImam Khomeini International University (IKIU)QazvinIslamic Republic of Iran

Personalised recommendations