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Quantification of β-carotene, lycopene, and chlorophyll content in tomato fruits of enrichment of chicken feathers composting

  • Mohan ArthanariEmail author
  • Senthilkumar Dhanapalan
Open Access
Short communication
  • 137 Downloads

Abstract

Purpose

Chicken feather was thrown into the environments, which causes health hazards and environmental pollution. It contains large number of proteins which can be converted into organic fertilizer to reduce the pollution load. These will help to minimize the protein needs and maintain low cost for utilization as raw material for manure production.

Methods

Chicken feather waste was subjected to composting by mixing it with cow dung and agricultural waste. Four different composts were prepared for composting of the chicken feather waste: E1, E2, E3, and E4, along with a control [cow dung (CW) + agricultural waste (AG)]. The major and minor nutrient contents of the compost were analyzed on the 90th day. The prepared compost was applied to Solanum lycopersicum plants; fruits pigments (lycopene, β-carotene, and chlorophyll) were evaluated.

Result

Chicken feather compost has high nutrient level organic carbon (1.83%), nitrogen (7.33%), potassium (4.40%), sulphur (19.69 ppm), zinc (4.96 ppm), boron (0.59 ppm), and iron (6.62 ppm) except phosphorus (0.26%) in the control. Results revealed highest lycopene in E4 (0.5881 mg/100 ml) and β-carotene in E1 (0.2699 mg/100 ml) when compared with the control value lycopene (0.4602 mg/100 ml) and β-carotene (0.1341 mg/100 ml). A positive correlation has been established between lycopene/β-carotene and negative correlation have been existing chlorophyll content vs lycopene/β-carotene.

Conclusion

Chicken feather can be eco-friendly when converted into compost which in turn enriches the quality of tomato fruits (lycopene and β-carotene).

Keywords

β-Carotene Lycopene Total chlorophyll Chlorophyll a and b 

Introduction

Poultry farms produce a lot of different types of waste products, such as fecal excreta, bedding material, remaining feed, departed chickens, damaged eggs, and packing materials. Kelleher et al. (2002) stated that poultry wastes are also found in conveyor belts, cleaning systems and carrying materials. Globally, 8.5 billion tons of feathers are generated annually, with approximately 350 million tons generated in India alone (Agrahari and Wadhwa 2010). This poultry feather meal is a readily available and low-cost source of nitrogen (up to 15% N) and functions as a potential biofertilizer (Jeong et al. 2010). Poultry feathers are animal by-products with high protein content, which contain leucine, valine, arginine, isoleucine, phenylalanine, and threonine, but with smaller proportions of lysine, methionine, histidine, and tryptophan (Yokote et al. 2007; Bandegan et al. 2010). The macro- and micronutrients present in the chicken feather waste-compost retain the inorganic fertilizer (Kalaivanan and Hattab 2016), it also improved the physicochemical and biological properties of the soil (Adhami et al. 2014; Lim et al. 2015). A huge volume of chicken feather waste causes environmental problems, contaminates air, water, and soil ecosystem. Soil microbes, in turn, ferment them into organic nitrogen and may be utilized for agricultural purposes (Paul et al. 2018).

Tomato (Solanum lycoperscium) is a major vegetable crop and commonly cultivated worldwide. Lycopene is one of the major (90%) important natural carotenoids found in tomato, which is the main pigment that gives the red color to tomatoes (Shi et al. 1999). Carotenoids, such as β-carotene and lycopene, are important components of antioxidant defense against lipid peroxidation in living cells (Agarwal et al. 2005). Lycopene contains unsaturated acyclic carotenoid, with 11 conjugated double bonds that act as effective free radical scavengers (Shi and Maguer 2000). Carotenoids are important dietary sources of vitamin A, after β-carotene retinol bioconversion into pro-vitamin A (Tang 2010). The objective of this present research work was to manage chicken feather-waste through a mixture of aerobic decomposition processes. The influence of treated chicken feather wastes on the tomato fruits β-carotene, lycopene, and chlorophyll contents, and estimation of chicken feathers waste to be used as organic nitrogen enriched fertilizers.

Materials and methods

Collection of raw materials

Chicken feather (CF) waste has been collected from slaughterhouses in Elur village, Namakkal District of Tamil Nadu. The agricultural waste and cow dung were also collected from the same place. The collected CF were washed in tap water several times, sun-dried, cut into small pieces and utilized for compost experiments.

Experimental setup

Five circular containers (25 cm diameter and 40 cm height) had the raw materials (CD:AW:CF) and were mixed in a different ratios C (4:4:0), E1 (4:4:1), E2 (4:4:2), E3 (4:4:3), and E4 (4:4:4). The compost was maintained at 40–60% moisture level throughout the experimental period (90 days).

Physico-chemical analysis

pH of compost samples was determined using a double DH2O suspension of compost in the ratio of 1:10 (w/v) analyzed in Digital pH meter (Vasanthi et al. 2014), the total organic carbon (TOC) content was estimated using the method of Abdullah and Chin (2010). Total Nitrogen (TN) was estimated by Micro Kjeldahl method (Mohee et al. 2008; Unmar and Mohee 2008). Total Phosphorus (TP) was detected by the colorimetric method (John 1970). Total potassium (TK) was determined after digesting the sample in diacid mixture (concentrated HNO3; concentrated HCIO4, 4:1, v/v), by Flame Photometer (Bansal and Kapoor, 2000). Sulfur (S), zinc (Z), boron (B), and iron (Fe) were measured by the diacid digest using an atomic absorption spectrophotometer (Vasanthi et al. 2014).

Biochemical analysis of fruit pigments

1 g of tomato fruit was homogenized with (10–20 ml) of acetone–hexane (4:6) solvent. After homogenization, the supernatant was used for the biochemical analysis (Nagata and Yamashita 1992).

Quantification of lycopene, β-carotene, and chlorophyll

The content of lycopene, β-carotene, and chlorophyll was determined based on a spectrophotometric analysis according to the method described by Mackinney’s method and Kimura’s method. Lycopene, β-carotene, and chlorophyll a, b, and total contents were expressed as mg/100 ml.

Statistical analysis

Statistical analysis was performed and the data were compared using one-way ANOVA, Tukey’s HSD (honestly significant difference) and DMRT (Duncan multiple range test) in SPSS (version 21), Pearson—Correlation Matrix and Visualization—correlation matrix in R—STHDA (statistical tools for high-throughput data analysis).

Results and discussion

The results of the physicochemical test of the final products are presented in Table 1. The pH is one of the important characteristics of the composting process; the chicken feathers’ compost was highly alkaline (pH > 8.60). The chicken feathers composting occurs in a strongly alkaline environment during the process which causes the inactivation of the pathogens in the feathers compost (Tronina and Bubel 2008). The maximum available nitrogen content in E2 was 7.33%, which is consistent with the findings of Jeong et al. 2010. Nitrogen serves as an essential component of amino acid, the basic structural unit of proteins. Here the significant decrease in the nitrogen content of the compost was resultant of the ammonification process, which converted a fraction of organic NH3 into NH4+ ions (Tiquia, 2002). Phosphorus provided energy for plant growth and maintains the equilibrium of plant (McWilliams 2003). The feather compost contains phosphorus which is lesser than the control in our present study (0.26%). Phosphorus enhances the chlorophyll level in the plants. An elevated potassium (K) level was recorded in E3 (4.40%). Wool and hair waste has a nutrient source (N, P, K, and S) for crops and potential to improve soil biology and chemical properties (Valtcho and Zheljazkov 2005). The maximum of micronutrients was present in E1 compost: S 19.69; Zn 4.96; B 0.59 and Fe 15.53 ppm. The requirement of micronutrients is negligible and it may affect plant growth. Most of the micronutrient is present either in Fe form or as chelated EDTA in the metabolism (McWilliams 2003).
Table 1

Physicochemical parameter of feather compost samples

Samples

pH

OC (%)

N (%)

P (%)

K (%)

S (ppm)

Zn (ppm)

B (ppm)

Fe (ppm)

Control

8.32 ± 0.29c

0.84 ± 0.57d

1.23 ± 0.49e

0.26 ± 0.13a

2.76 ± 0.78e

14.79 ± 1.06d

1.77 ± 0.58d

0.41 ± 0.15e

10.60 ± 1.96b

E1

8.26 ± 0.99d

0.51 ± 0.08e

2.25 ± 0.74d

0.14 ± 0.07e

2.89 ± 1.01d

19.69 ± 1.08a

4.96 ± 0.80a

0.59 ± 0.16a

15.53 ± 0.88a

E2

8.60 ± 1.16a

1.73 ± 1.11b

7.33 ± 1.21a

0.16 ± 0.05b

3.82 ± 0.90b

14.55 ± 0.07e

1.81 ± 0.69b

0.45 ± 0.10d

5.20 ± 1.02e

E3

8.09 ± 1.33e

1.83 ± 1.14a

6.75 ± 0.89b

0.14 ± 0.01d

4.40 ± 1.30a

15.80 ± 2.49c

1.82 ± 0.19c

0.52 ± 0.29b

6.00 ± 2.55d

E4

8.37 ± 1.33b

1.55 ± 0.87c

6.35 ± 0.04c

0.15 ± 0.08c

3.62 ± 1.52c

16.80 ± 1.92b

1.40 ± 0.94e

0.51 ± 0.14c

6.62 ± 1.12c

Mean values designated with different superscripts indicate that differences between treatment are significant according to the Tukey’s HSD (honestly significant difference) and DMRT (Duncan multiple range test) (P < 0.05)

The pigment composition of tomato fruit was determined and the data are recorded in Table 2. The results revealed highest lycopene content recorded as E4 (0.5881 mg/100 ml), E3 (0.5803 mg/100 ml), E2 (0.5773 mg/100 ml), E1 (0.5388 mg/100 ml) and lowest in C (0.4602 mg/100 ml). Mendelova et al. (2013), found that supplemental nutrition by increased sulfur content was manifested by higher lycopene content in fruits by 44.5% for a tomato variety. High level of β-carotene was present in E1-treated plants and the level was reduced towards the control plant [E1 (0.2699 mg/100 ml), E3 (0.2681 mg/100 ml), E4 (0.2575 mg/100 ml), E2 (0.2699 mg/100 ml) and control (0.1341 mg/100 ml)]. Huang et al. (2010) observed the content of lycopene and β-carotene in tomato sauce and ketchup. The tomato fruits emerging from the control plants exhibit maximum level of chlorophyll (a) 0.0518 mg/100 ml and total chlorophyll 0.0904 mg/100, whereas chlorophyll (b) in E1 was 0.0466 mg/100 ml.
Table 2

Analysis of plant pigments lycopene, β-carotene, and chlorophyll

Experiment

Lycopene (mg/100 ml)

β-Carotene (mg/100 ml)

Chlorophyll (a) (mg/100 ml)

Chlorophyll (b) (mg/100 ml)

Total chlorophyll (mg/100 ml)

Control

0.4602

0.1341

0.0518

0.0386

0.0904

E1

0.5388

0.2699

0.0343

0.0466

0.0809

E2

0.5773

0.2519

0.0159

0.0139

0.0298

E3

0.5803

0.2681

0.0177

0.0342

0.0519

E4

0.5881

0.2575

0.0114

0.0241

0.0355

Correlation matrix

To determine the relationships among the analyzed traits, a Pearson correlation matrix analysis was performed as shown in Table 3. A high positive correlation was established between lycopene and β-carotene (r = 0.9*), and negative correlation against chlorophyll contents (lycopene r = − 0.55, − 0.98** and − 0.87; β-carotene r = − 0.19, − 0.8 and − 0.59). A positive correlation was found within the chlorophyll content of tomato fruits with a high positive correlation coefficient (r = 0.94*) between total chlorophyll and chlorophyll (a). Visualization of the correlation matrix was plotted using corrplot package. In Correlogram Fig. 1, the positive and negative correlations are displayed in blue and red color. Correlation coefficients were detected by the proportion of color intensity and size of the circle. The positive correlation between total chlorophyll and chlorophyll A highly correlated in the blue color circle. Chlorophyll A and lycopene are mostly negatively correlated with the red color circle. No correlation was established between lycopene and total chlorophyll.
Table 3

Correlation matrix of lycopene, β-carotene and chlorophyll

 

Lycopene

β-Carotene

Chlorophyll B

Chlorophyll A

Total chlorophyll

Lycopene

1

    

β-Carotene

0.9*

1

   

Chlorophyll B

− 0.55

− 0.19

1

  

Chlorophyll A

− 0.98**

− 0.8

0.67

1

 

Total chlorophyll

− 0.87

− 0.59

0.89*

0.94*

1

*Correlation is significant at the 0.05 level

**Correlation is significant at the 0.01 level

Fig. 1

Correlogram. Ly_pn lycopene, Ca_tn β-carotene, Ch_B chlorophyll b, Ch_A chlorophyll a, Ch_T total chlorophyll

Conclusion

Based on the result obtained, we can conclude that the various compost mixtures exhibit statistical significance among pigments of tomato fruits. The highest content of β-carotene and lycopene was observed in E1 and E5 tomato fruits.

Notes

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Authors and Affiliations

  1. 1.Department of ZoologyKandaswami Kandar’s CollegeNamakkalIndia

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