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A Review on Alternative Carbon Sources for Biological Treatment of Nitrate Waste

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Abstract

Huge amount of wastewater containing nitrogen is produced by various chemical and biological industries. Nitrogen is present in the form of ammonia, nitrate and nitrite. This review deals with treatment of nitrate based effluent using biological denitrification. Because of its adverse effect on aquatic life and human health, treatment of nitrate bearing effluents has become mandatory before discharge. Treatment of such wastes is a liability for the industries and incurs cost. However, the economics of the process can be controlled by selection of proper method and reduction in the operating cost. This paper reviews the advantages and disadvantages of different methods of nitrate removal with emphasis on biological denitrification. The cost of biological denitrification is controlled by the carbon source. Hence, use of alternative carbon sources such as agricultural wastes, industrial effluent or by products is reviewed in this paper. Policies for reducing the cost of nitrate treatment and enhancing the efficiency have been recommended.

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References

  1. P.B. Dhamole, R.R. Nair, S.F. D’Souza, S.S. Lele, Simultaneous removal of carbon and nitrate in an airlift bioreactor. Bioresour. Technol. 100, 1082–1086 (2009)

    Article  Google Scholar 

  2. P.B. Dhamole, R.R. Nair, S.F. D’Souza, S.S. Lele, Denitrification of highly alkaline nitrate waste using adapted sludge. Appl. Biochem. Biotechnol. 151, 433–440 (2008)

    Article  Google Scholar 

  3. P.M. Biradar, P.B. Dhamole, R.R. Nair, S.B. Roy, S.K. Satpati, S.F. D’Souza, S.S. Lele, A.B. Pandit, Long-term stability of biological denitrification process for high strength nitrate removal from wastewater of uranium industry. Environ. Prog. 27, 365–372 (2008)

    Article  Google Scholar 

  4. R.R. Nair, P.B. Dhamole, S.S. Lele, S.F. D’Souza, Biotreatment of high strength nitrate waste using immobilized pre-adapted sludge. Appl. Biochem. Biotechnol. 151, 193–200 (2008)

    Article  Google Scholar 

  5. P.B. Dhamole, R.R. Nair, S.F. D’Souza, S.S. Lele, Denitrification of high strength nitrate waste. Bioresour. Technol. 98, 247–252 (2007)

    Article  Google Scholar 

  6. R.R. Nair, P.B. Dhamole, S.S. Lele, S.F. D’Souza, Biological denitrification of high strength nitrate waste using preadapted denitrifying sludge. Chemosphere 97, 1612–1617 (2007)

    Article  Google Scholar 

  7. C. Glass, J. Silverstein, Denitrification kinetics of high nitrate concentration water: pH effect on inhibition and nitrite accumulation. Water Res. 32, 831–839 (1998)

    Article  Google Scholar 

  8. C. Glass, J. Silverstien, Denitrification of high nitrate, high salinity wastewater. Water Res. 33, 223–229 (1999)

    Article  Google Scholar 

  9. D. Bilanovic, P. Battistoni, F. Cecchi, P. Pavan, J. Mata-Alvarez, Denitrification under high nitrate concentration and alternating anoxic conditions. Water Res. 33, 3311–3320 (1999)

    Article  Google Scholar 

  10. C.W. Francis, C.W. Hatcher, Biological denitrification of high nitrate wastes generated in the nuclear industry, in Biological Fluidized Bed Treatment of Water and Wastewater, ed. by P.F. Cooper, B. Atkinson (Ellis Horwood Ltd, Chichester, 1980)

    Google Scholar 

  11. P.B. Dhamole, Studies in airlift bioreactor for effluent treatment. PhD Thesis submitted to the University of Mumbai, 2006

  12. U.S. Environmental Protection Agency, 1987. Nitrate/Nitrite: Health Advisory, Office of Drinking Water, U.S. Environmental Protection Agency. Washington D.C., March 31

  13. D. Majumdar, N. Gupta, Nitrate pollution of ground water and associated human health disorders. Indian J. Environ. Health 42, 28–39 (2000)

    Google Scholar 

  14. M. Shrimali, K. Singh, New methods of nitrate removal from water. Environ. Pollut. 112, 351–359 (2001)

    Article  Google Scholar 

  15. P.M. Newborne, Nitrates and nitrites in food and in the biological system, in Trace Substances and Health. A Hand Book Pt-2, ed. by P.M. Newborne (Marcel Dekker, New York, 1982), pp. 1–46

    Google Scholar 

  16. T.Y.K. Chan, Food borne nitrates and nitrites as a case of methaemoglobinemia. Southeast Asian J. Trop. Public Health 27, 189–192 (1996)

    Google Scholar 

  17. I.D. Benefield, J.F. Judikins, B.L. Weand, Process Chemistry for Water and Wastewater Treatment (Prentice Hall Inc, Englewood Cliffs, 1982). 449

    Google Scholar 

  18. D. Forman, Nitrate exposure and human cancer.in Nitrate Contamination, RD NATO ASI Series, eds.I. Bogardi, R. Kuzelka, vol. G. 30. (Springer,New York, 1991).

  19. Y. Richard, Denitrification of water for human consumption. Prog. Water Technol. 12, 173 (1980)

    MathSciNet  Google Scholar 

  20. R.K. Trivedi, P.K. Goel, Chemical and Biological Methods for Water Pollution Studies (Environmental Publications, Karad, 1984)

    Google Scholar 

  21. P.J. Bliss, D. Barnes, Biological nitrogen control in wastewaters. Effl. Water Treat. J. 21, 65–74 (1981)

    Google Scholar 

  22. A. Kapoor, T. Viraraghavan, Nitrate removal from drinking water—a review. J. Environ. Eng. 123, 374–380 (1997)

    Article  Google Scholar 

  23. F.J. Frost, K. Tollestrup, G.F. Craun, R. Raucher, J. Stomp, J. Chwirka, Evaluation of costs and benefits of lower arsenic MCL. JAWWA 94, 71–80 (2002)

    Google Scholar 

  24. B.-U. Bae, Y.-H. Jung, W.-W. Han, H.-S. Shin, Improved brine recycling during nitrate removal using ion exchange. Water Res. 36, 3330–3340 (2002)

    Article  Google Scholar 

  25. S. Horold, K.D. Vorlop, T. Tacke, M. Sell, Development of catalysts for a selective nitrate and nitrite removal from drinking water. Catal. Today 17, 21–27 (1993)

    Article  Google Scholar 

  26. J. Batista, A. Pintar, M. Ceh, Kinetics of the catalytic liquid-phase hydrogenation of aqueous nitrate solution. Catal. Lett. 43, 79 (1997)

    Article  Google Scholar 

  27. M. Prusse, S. Horold, K.D. Vorlop, Effect of the preparation conditions on catalytic properties of bimetallic catalysts for nitrate removal from water. Chem. Ing. Technol. 69, 93 (1997)

    Article  Google Scholar 

  28. K.D. Vorlop, M. Prusse, Catalytic removing nitrate from water. Catal. Sci Ser. 1, 195 (1999)

    Article  Google Scholar 

  29. Y. Matatov-Meytal, V. Barelko, I. Yuranov, M. Sheintuch, Cloth catalysts in water denitrification I. Pd on glass fibres. Appl. Catal. B Environ. 27, 127–135 (2000)

    Article  Google Scholar 

  30. F. Hell, J. Lahnsteiner, H. Frischherz, G. Baumgartner, Experience with full-scale electrodialysis for nitrate and hardness removal. Desalination 117, 173–180 (1998)

    Article  Google Scholar 

  31. J.J. Schoeman, A. Steyn, Nitrate removal with reverse osmosis in a rural area in South Africa. Desalination 155, 15–26 (2003)

    Article  Google Scholar 

  32. USEPA October 2000. Arsenic removal from drinking water by ion exchange and activated alumina plants, EPA/600/R-00/088 October 2000

  33. G.A. Guter, Nitrate removal from contaminated groundwater by anion exchange, in Ion Exchange Technology Advances in Pollution Control, ed. by A.K. Sengupta (Technomic Publishing Co Inc, Lancaster, 1997), pp. 61–113

    Google Scholar 

  34. S. Samatya, N. Kabay, U. Yuksel, M. Arda, M. Yuksel, Removal of nitrate from aqueous solution by nitrate selective ion exchange resins. React. Funct. Polym. 66, 1206–1214 (2006)

    Article  Google Scholar 

  35. K.J. Reddy, J. Lin, Nitrate removal from groundwater using catalytic reduction. Water Res. 34, 995–1001 (2000)

    Article  Google Scholar 

  36. S. Horold, T. Tacke, K.D. Vorlkop, Catalytic removal of nitrate and nitrite from drinking water—1: screening for hydrogenation catalysis and influence of reaction conditions on activity and selectivity. Environ. Technol. 14, 931–939 (1993)

    Article  Google Scholar 

  37. Y. Matatov-Meytal, M. Sheintuch, Catalytic abatement of water pollutants. Ind. Chem. Eng. Res. 36, 326–343 (1998)

    Google Scholar 

  38. J.L. Ingraham, Microbiology and genetics of denitrifiers, in Denitrification, Nitrification and Atmospheric Nitrous Oxide, ed. by C.C. Delwiche (Wiley, New York, 1981), pp. 45–65

    Google Scholar 

  39. V. Mateju, S. Cizinska, J. Krejei, T. Janoch, Biological water denitrification—a review. Enzym. Microb. Technol. 14, 170–183 (1992)

    Article  Google Scholar 

  40. D.V. MacDonald, Denitrification by fluidized biofilm reactor. Water Sci. Technol. 22, 451–461 (1990)

    Google Scholar 

  41. C.D. Rocca, Meric S. Belgiorno, Overview of in situ applicable nitrate removal processes. Desalination 204, 46–62 (2007)

    Article  Google Scholar 

  42. N.R. Louzeiro, D.S. Mavinic, W.K. Oldham, A. Meisen, I.S. Gardner, Methanol induced biological nutrient removal kinetics in a full-scale sequencing batch reactor. Water Res. 36, 2721–2732 (2002)

    Article  Google Scholar 

  43. L. Foglar, F. Briski, Wastewater denitrification process- the influence of methanol and kinetic analysis. Process Biochem. 39, 95–103 (2003)

    Article  Google Scholar 

  44. L. Foglar, F. Briski, L. Sipos, M. Vukovic, High nitrate removal from synthetic wastewater with the mixed bacterial culture. Bioresour. Technol. 96, 879–888 (2005)

    Article  Google Scholar 

  45. B. Ovez, Batch biological denitrification using Arundo donax, Glycyrrhiza glabra, and Gracilaria verrucosa as carbon source. Process Biochem. 41, 1289–1295 (2006)

    Article  Google Scholar 

  46. M.I.M. Soares, A. Abeliovich, Wheat straw as substrate for water denitrification. Water Res. 32, 3790–3794 (1998)

    Article  Google Scholar 

  47. S. Aslan, A. Turkman, Simultaneous biological removal of endosulfan (α + β) and nitrates from drinking waters using wheat straw as substrate. Environ. Int. 30, 449–455 (2004)

    Article  Google Scholar 

  48. M. Volokita, A. Abeiovichm, M. Ines, M. Saoares, Denitrification of ground water using cotton as energy source. Water Sci. Technol. 34, 379–385 (1996)

    Article  Google Scholar 

  49. M. Volokita, S. Belkin, A. Abeiovichm, M. Saoares, Biological denitrification of drinking water using newspaper. Water Res. 30, 965–971 (1996)

    Article  Google Scholar 

  50. Z.X. Quan, Y.S. Jin, C.R. Yin, J.J. Lee, S.T. Lee, Hydrolyzed molasses as an external carbon source in biological nitrogen removal. Bioresour. Technol. 96, 1690–1695 (2005)

    Article  Google Scholar 

  51. A. Asoy, H. Odegaard, K. Bach, R. Pujol, M. Hamon, Denitrification in a packed bed reactor (BIOFOR)—experiments with different carbon sources. Water Res. 32, 1463–1470 (1998)

    Article  Google Scholar 

  52. B. Ovez, S. Ozgen, M. Yuksel, Biological denitrification in drinking water using Glycyrrhiza glabra and Arunda donax as the carbon source. Process Biochem. 41, 1539–1544 (2006)

    Article  Google Scholar 

  53. D.L. Kaplan, P.A. Riley, J. Pierce, A.M. Kaplan, Denitrification of high nitrate loads- efficiencies of alternative carbon source. Int. Biodeterior. 23, 233–248 (1987)

    Article  Google Scholar 

  54. P.J.W. Ten Have, H.C. Willers, P.J.L. Derikx, Nitrification and denitrification in an activated sludge system for supernatant from settled sow manure with molasses as an extra carbon source. Bioresour. Technol. 47, 135–141 (1994)

    Article  Google Scholar 

  55. R.A.R. Boaventura, A.E. Rodrigues, Denitrification kinetics in rotating disk biofilm reactor. Chem. Eng. J. 65, 227–235 (1997)

    Article  Google Scholar 

  56. G.D. Najafpour, C.P. Shan, Enzymatic hydrolysis of molasses. Bioresour. Technol. 86, 91–94 (2003)

    Article  Google Scholar 

  57. S.I. Lee, J.H. Park, K.B. Ko, B. Kooman, Effect of fermented swine wastes on biological nutrient removal in sequencing batch reactors. Water Res. 31, 1807–1812 (1997)

    Article  Google Scholar 

  58. D. Obaja, S. Mace, J. Mata-Alvarez, Biological nutrient removal by a sequencing batch reactor (SBR) using an internal organic carbon source in digested piggery wastewater. Bioresour. Technol. 96, 7–14 (2005)

    Article  Google Scholar 

  59. Y. Xu, Volatile fatty acids carbon source for biological denitrification. J. Environ. Sci. 8, 257–268 (1996)

    Google Scholar 

  60. H. Constantin, M. Fick, Influence of C-sources on the denitrification rate of a high–Nitrate concentrated industrial wastewater. Water Res. 31, 583–589 (1997)

    Article  Google Scholar 

  61. C. Sans, J. Mata-Alvarez, F. Cecchi, P. Pavan, Volatile fatty acids production by mesophilic fermentation of mechanically sorted urban organic waste in a plug flow reactor. Bioresour. Technol. 51, 89–96 (1995)

    Article  Google Scholar 

  62. P. Elefsiniotis, D.G. Wareham, M.O. Smith, Use of volatile fatty acids from an acid-phase digester for denitrification. J. Biotechnol. 114, 289–297 (2004)

    Article  Google Scholar 

  63. P. Elefsiniotis, D. Li, The effect of temperature and carbon source on denitrification using volatile fatty acids. Biochem. Eng. 28, 148–155 (2006)

    Article  Google Scholar 

  64. S.I. Lee, B. Koopman, S.K. Park, K. Cadee, Effect of fermented wastes on denitrification in activated sludge. Water Environ. Res. 67, 1119–1122 (1995)

    Article  Google Scholar 

  65. J.L. Barnard, Design of prefermentation processes, in Design and Retrofit of Wastewater Treatment Plants for Biological Nutrient Removal, ed. by C.W. Randall, J.L. Barnard, H.D. Stensel (Technomic, Lancaster, 1992), pp. 85–96

    Google Scholar 

  66. J. Barlindhaug, H. Odegaard, Thermal hydrolysate as carbon source for denitrification. Water Sci. Technol. 33, 99–108 (1996)

    Article  Google Scholar 

  67. J.C. Akunna, C. Bizeau, R. Moletta, Nitrate and nitrite reductions with anaerobic sludge using various carbon source; glucose, glycerol, acetic acid, lactic acid and methanol. Water Res. 27, 1303–1312 (1993)

    Article  Google Scholar 

  68. J.P. Rajapakse, J.E. Scutt, Denitrification with natural gas and various new growth media. Water Res. 33, 3723–3734 (1999)

    Article  Google Scholar 

  69. M. Waki, K. Suzuki, T. Osada, Y. Tanaka, Methane-dependent denitrification by a semi-partitioned reactor supplied separately with methane and oxygen. Bioresour. Technol. 96, 921–927 (2005)

    Article  Google Scholar 

  70. F. Thalasso, A. Vallecillo, P. Garcı´a-Encina, F. Fdz-Polanco, The use of methane as a sole carbon source for wastewater denitrification. Water Res. 31, 55–60 (1997)

    Article  Google Scholar 

  71. E. Houbron, M. Torrijos, B. Capdeville, An alternative use of biogas applied at the water denitrification. Water Sci. Technol. 40, 115–122 (1999)

    Article  Google Scholar 

  72. Y. Honda, Z. Osawa, Microbial denitrification of wastewater using biodegradable polycaprolactone. Polym. Degrad. Stab. 76, 321–327 (2002)

    Article  Google Scholar 

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Dhamole, P.B., D’Souza, S.F. & Lele, S.S. A Review on Alternative Carbon Sources for Biological Treatment of Nitrate Waste. J. Inst. Eng. India Ser. E 96, 63–73 (2015). https://doi.org/10.1007/s40034-014-0055-8

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