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Formulation and Assessment of In Vitro Antimicrobial Activity of Herbal Toothpaste

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The oral health problems mainly dental caries and periodontal diseases have been considered very serious. Even though the availability of many antimicrobial agents, these are basically chemicals by virtue they alter the oral micro-biota and have unwanted side effects such as tooth staining, vomiting, diarrhoea and development of antimicrobial resistance. The objective of the present investigation was to develop the herbal toothpaste containing pomegranate peel extract and clove oil for prevention and treatment of dental caries and periodontal diseases. Nine herbal toothpastes (TN1 to TN9) were prepared containing different concentrations of pomegranate peel extract (0.2–1.8% w/w) and clove oil (1–1.4% w/w) and subjected for pH, spreadability, foaming power, fineness, tube extrudability and homogeneity tests. The in vitro antimicrobial activity studies were performed using standard strains like Candida albicans, Streptococcus mutans and Staphylococcus aureus. The marketed toothpaste was used as standard control in all the studied parameters. All the prepared formulations (TN1 to TN9) were showed good results in the studied parameters, and results were comparable with standard control. Among the prepared formulations TN7 (1.4% w/w pomegranate peel extract and 1% w/w clove oil) showed optimum results. In in vitro studies the formulations TN7 showed good zone inhibition against C. albicans, S. mutans and S. aureus with diameter 26, 27 and 25 mm, respectively. In conclusion, herbal toothpaste containing pomegranate peel extract and clove oil displayed promising antimicrobial activity against C. albicans, S. mutans and S. aureus. The prepared herbal toothpaste can help to prevent dental caries and periodontal diseases.

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Acknowledgements

We would like to thank Head of Department of Microbiology Ashwini Sahakari Rural Medical College and Research Centre, Solapur, India, for providing micro-organisms and helped us to carry out antimicrobial studies effectively. This research did not receive any grant from any funding agencies.

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Authors and Affiliations

  1. Department of Pharmaceutics, D.S.T.S. Mandal’s College of Pharmacy, Solapur, Maharastra, 413004, India

    Baburao N. Chandakavathe

  2. Department of Pharmaceutics, Shri Vithal Education & Research Institute’s, College of Pharmacy, Pandharpur, Maharastra, 413304, India

    Baburao N. Chandakavathe

  3. Department of Pharmaceutical Chemistry, Bharati Vidyapeeth’s Poona College of Pharmacy, Pune, Maharastra, 411038, India

    Ravindra G. Kulkarni

  4. Department of Pharmacology, D.S.T.S. Mandal’s College of Pharmacy, Solapur, Maharastra, 413004, India

    Shivsharan B. Dhadde

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  1. Baburao N. Chandakavathe
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Correspondence to Baburao N. Chandakavathe.

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Significance statement: Dental diseases are major oral health problems in the world. Present investigation data suggest that toothpaste containing pomegranate peel extract and clove oil plays very important role in the prevention and treatment of dental caries and periodontal diseases.

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Chandakavathe, B.N., Kulkarni, R.G. & Dhadde, S.B. Formulation and Assessment of In Vitro Antimicrobial Activity of Herbal Toothpaste. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 93, 317–323 (2023). https://doi.org/10.1007/s40011-022-01424-5

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  • DOI: https://doi.org/10.1007/s40011-022-01424-5

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