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Anti-allergic and Anti-hyperglycemic Potentials of Lumnitzera racemose Leaves: In vivo and In silico Studies

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Lumnitzera racemosa a mangrove plant locally used to treat asthma, diabetes, herpes and hypertension. Present study was designed to investigate the anti-allergic and anti-hyperglycemic activities and also the responsible polyphenols for these potentials. Anti-allergic activity was evaluated on toluene 2,4-diisocyanate (TDI)-induced allergic model mice and anti-diabetic activity on streptozotocin (STZ)-induced diabetic mice. Natural polyphenolic compounds were determined by HPLC–DAD. In addition, in silico molecular docking was conducted via ‘Vina Wizard’ program in PyRx–Python Prescription 0.8 on nine isolated compounds against histamine H1 receptor (H1R) and sulfonylurea receptor 1 (SUR1). Acute toxicity study confirmed the safety of the leaves extract up to 3000 mg/kg dose in mice. Different biochemical parameters were also in normal at a dose of 500 mg/kg in sub-acute toxicity study. The leaves extract significantly suppressed (P < 0.05) TDI-induced allergy-like symptoms. The total and differential counts of WBC in blood and broncho alveolar lavage fluid (BALF) were also decreased in extract treated allergic mice. In STZ-induced diabetic mice, the extract significantly reduced the level of blood glucose and different related biomarkers. HPLC profiling revealed the presence of nine phenolic compounds; among these myricetin and rosmarinic acid demonstrated the best docking score − 8.4 kcal/mol against H1R whereas − 7.3 kcal/mol for standard drug cetirizine. On the other hand, rutin hydrate exhibited best docking score − 10.2 kcal/mol against SUR1, whereas standard drug glibenclamide showed − 8.4 kcal/mol. In conclusion, L. racemosa is safe and rich in different polyphenolic compounds which were found effective against allergy and diabetes.

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Abbreviations

EELR:

Ethanolic extract of Lumnitzera racemosa

TDI:

Toluene 2, 4-diisocyanate

STZ:

Streptozotocin

OECD:

Organization for economic co-operation and development

H1R:

Histamine H1 receptor

SUR1:

Sulfonylurea receptor 1

SGOT:

Serum glutamic oxaloacetic transaminase

SGPT:

Serum glutamic pyruvic transaminase

ALP:

Alkaline phosphatase

TG:

Triglyceride

SEM:

Standard error of mean

vs:

Versus

RMSD:

Root-mean-square deviation

UB:

Upper bound

LB:

Lower bound

PDB:

Protein data bank

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Acknowledgements

The authors are grateful to the Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh, for providing experimental mice. The authors would like to express their heartiest thanks and gratitude to Chemical Research Division, BCSIR Laboratories, Dhaka, Bangladesh, for helping in HPLC analysis.

Funding

The work was supported by the Khulna University Research cell, Khulna-9208, Bangladesh (Grant Number: KURC- 04/2000-69).

Author information

Authors and Affiliations

  1. Pharmacy Discipline, Khulna University, Khulna, 9208, Bangladesh

    Rabindra Nath Acharyya, Shuvra Mithila, Suborna Rani, Md. Arman Islam, Mimi Golder, Shrabanti Dev & Asish Kumar Das

  2. Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh

    Khondoker Shahin Ahmed & Hemayet Hossain

Authors
  1. Rabindra Nath Acharyya
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  2. Shuvra Mithila
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  3. Suborna Rani
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  4. Md. Arman Islam
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Contributions

The submitted research work was conducted in collaboration among the authors. The project was designed by AKD (Asish Kumar Das) and SD (Shrabanti Dev). SM (Shuvra Mithla) and RNA (Rabindra Nath Acharyya) conducted the detail literature review and performed the phytochemical screening. SM, SR (Suborna Rani) and RNA have performed the extraction, acute and sub-acute toxicity study, anti-allergic and anti-hyperglycemic activity study. HPLC analysis was performed by KSA (Khondoker Shahin Ahmed) and HH (Hemayet Hossain). SD carried out the statistical analysis. Article writing and in silico molecular docking were done by MAI (Md. Arman Islam), RNA and MG (Mimi Golder). Critical revision of the article was done by SD and AKD. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Asish Kumar Das.

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Conflict of interest

The authors declared that they have no conflict of interest.

Ethical Approval

Animal Ethics Committee, Life Science School, Khulna University, Khulna-9208, Bangladesh, approved all experiments using mice (KU/PHARM/AEC/15/06/31).

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Significance Statement This study ensured the safety as well as anti-allergic and anti-diabetic potentials of L. racemosa leaves extract and also revealed that myricetin, rosmarinic acid and rutin hydrate might be the lead compounds for these therapeutic activities.

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Acharyya, R.N., Mithila, S., Rani, S. et al. Anti-allergic and Anti-hyperglycemic Potentials of Lumnitzera racemose Leaves: In vivo and In silico Studies. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 93, 147–158 (2023). https://doi.org/10.1007/s40011-022-01399-3

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