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Isolation and Molecular Characterization of MDV-1 from Vaccinated Poultry Birds in Haryana State of India

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Abstract

Marek’s disease (MD) is a lymphoproliferative and neuropathic disease of domestic fowl caused by an oncogenic Gallid Herpesvirus-2 (GaHV-2), also known as Marek’s Disease Virus serotype 1 (MDV-1). A total of forty (n = 40) liver samples of 14 outbreaks at poultry farm suspected for MD were investigated. Collected samples were processed for classical as well as for molecular diagnosis and further characterization of pathogens for the control of the disease. The Meq gene-based real-time PCR, nested PCR and virus isolation in CEF were performed. Out of 40 biological samples, 80% (32/40) samples were detected positive for MDV-1 by real-time PCR, 67.5% (27/40) by nested PCR and only 25.9% (7/27) by virus isolation in CEF. The nucleotide and deduced amino acid sequence data analysis of Meq gene revealed the presence of virulent MDV-1 strain in the majority of the samples of outbreaks. The mild virulent strain was also confirmed in only one field samples with maximum sequence identity with vaccine strain from the Netherlands (CVI988) and China (814). Present study conclude that the virulent strain of MDV-1 is circulating in Haryana state of India and responsible for morbidity and mortality among vaccinated poultry birds.

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Acknowledgements

The poultry farmers, field veterinarians and non-teaching staff of the Department of Animal Biotechnology are acknowledged for their help in the study. We acknowledge funding support from LUVAS Hisar.

Funding

LUVAS, State Scheme Number 121. 

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Authors and Affiliations

  1. Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

    Aman Kumar, Kanisht Batra, Koushlesh Ranjan & Sushila Maan

  2. Department of Animal Nutrition, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

    N. S. Maan

  3. Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

    N. K. Mahajan

  4. Department of Veterinary Physiology and Biochemistry, SVP University of Agriculture and Technology, Meerut, Uttar Pradesh, India

    Koushlesh Ranjan

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  1. Aman Kumar
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Correspondence to Aman Kumar.

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Significance statement: Marek’s disease (MD) is a lymphoproliferative and neuropathic disease of domestic fowl caused by an oncogenic Gallid Herpesvirus-2 (GaHV-2), also known as Marek’s Disease Virus serotype 1 (MDV-1). Present study has explained the circulation of virulent strain of MDV-1, responsible for morbidity and mortality among vaccinated poultry birds in Haryana state of India. It has not been published elsewhere and that it has not been submitted simultaneously for publication elsewhere.

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Kumar, A., Maan, N.S., Mahajan, N.K. et al. Isolation and Molecular Characterization of MDV-1 from Vaccinated Poultry Birds in Haryana State of India. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 92, 679–690 (2022). https://doi.org/10.1007/s40011-022-01363-1

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  • DOI: https://doi.org/10.1007/s40011-022-01363-1

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