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In Vitro Conservation Strategies for Sustainable Production of Secondary Metabolites in Psoralea corylifolia L.

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Psorelia corylifolia L. is a rich source of flavonoids, phenolics, antioxidants particularly psoralen with its significant biological activities. The plant products have great market demand on account of their versatile medicinal properties. Overexploitation from the wild and poor seed germination ability has enormously depleted its natural populations. Micropropagation is unconventional approach for its conservation and to fill market demand of its secondary metabolites. The present work was aimed to develop an efficient in vitro protocol for its rapid multiplication and large-scale production of secondary metabolites. Different phytohormonal combinations in Murashige and Skoog (MS) medium were used for shoot and callus induction. Maximum shoot induction (85.0%) through mature meristem culture was observed on MS medium supplemented with 1.5 mg/l BAP and 0.5 mg/l NAA. The callus induction and profuse shoot multiplication protocol was developed from leaf explants. MS medium with 1.5 mg/l BAP and 0.5 mg/l NAA was the best medium for maximum in vitro response of callus (85.0%), shooting and multiple shoots (7.0) with 8.5 cm length. A comparative analysis of psoralen content using HPLC, antioxidant properties, total flavonoid and phenolic contents was observed in field-grown seeds and in vitro raised callus. All these parameters were significantly higher in in vitro raised callus than field-grown seeds. The present investigation can serve as a prospective guide for in vitro regeneration, consequently for conservation and sustainable production of secondary metabolites from P. corylifolia.

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7. References

  1. Rao GV, Annamalai T, Kavitha Mukhopadhyay T (2010) Chemical examination and biological studies on the seeds of P corylifoliaLinn. Research Journal of Chemical Sciences. 2(1):50–58

    Google Scholar 

  2. Mishra S., Bhiashajya Ratnavali Chaukhambha (2009). Publication Varanasi Edition (54) 302–310

  3. The wealth of india, Raw materials CSIR., New Delhi (1995). viii,295–298.

  4. Rajpal V. (2005). Eastern Publishers;. Standardization of Botanicals; Vol. 2. New Delhi pp. 284–95.

  5. An H-J, Seo M-J, Choi I-Y, Park R-K, Jeong S, Lee J-Y, Kim H-M, Um J-Y, Hong S-H (2008) Induction of nitric oxide and tumour necrosis factor-α by Psoralea Corylifolia. Indian J Med Res 128:752–758

    PubMed  Google Scholar 

  6. Zhao L.H., Wu M.H., Xiang BR. (2005). Analysis of P. corylifoliaL. fruits in different regions. Chem Pharm Bull (Tokyo).

  7. Cheng Z.W., Cai X.F., Dat N.T., Hong S.S., Han A.R., Seo E.K., Hwang B.Y., Nan J.X., Lee D., Lee J.J. (2007). Bisbakuchiols A and B, novel dimeric meroterpenoids from Psoralea corylifolia.Tetrahedron Lett., 48, 8861–8864.Chinese J. Biotech., 24(5): 711–716.

  8. Dev S, Nayak UR, Mehta G (1973) Monoterpenoids- I, P. corylifoliaLinn. - Bakuchiol, A novel monoterpene phenol. Tetrahedron 29:1119–1125

    Article  Google Scholar 

  9. Prasad NR, Anand C, Balasubramanian S, Pugalendi KV (2004) Antidermatophytic activity of extracts from P. corylifolia(Fabaceae) correlated with the presence of a flavonoid compound. J Ethnopharmacol 91:21–24

    Article  CAS  Google Scholar 

  10. Tang SY, Whiteman M, Peng ZF, Jenner A, Yong EL, Halliwell B (2004) Characterization of Antioxidant and Antiglycation properties and isolation of active ingredients from traditional chinese medicines. Free Radical Biol Med 36(12):1575–1587

    Article  CAS  Google Scholar 

  11. Pae HO, Cho H, Oh GS, Kim NY, Song EK, Kim YC (2001) Bakuchiol from P. corylifoliainhibits the expression of inducible nitric oxide synthase gene via the inactivation of nuclear transcription factor kB in RAW 264.7 macrophages. International Journal of Immunopharmacology 1:1849–1855

    Article  CAS  Google Scholar 

  12. Qamaruddin A, Parveen N, Khan NU, Singhal KC (2003) Potential antifilarial activity of the leaves and seeds extracts of P. corylifoliaon cattle filarial parasite Setariacervi. Journal of Ethnopharmacology 84(2–3):313

    Article  Google Scholar 

  13. Zhang CZ, Wang SX, Zhang Y, Chen JP, Liang XM (2005) In vitro estrogenic activities of Chinese medicinal plants traditionally used for the management of menopausal symptoms. J Ethnopharmacol 98:295–300

    Article  CAS  Google Scholar 

  14. Latha PG, Evans DA, Panikkar KR, Jayavardhanan KK (2000) Immunomodulatory and antitumor properties of P. corylifoliaseeds. Fitoterapia 71:223–231

    Article  CAS  Google Scholar 

  15. Gidwani B, Alsapure RN, Duragkar NJ (2011) Pharmacognostic and standardization and physico-chemical evaluation of P. corylifoliaLinn seeds”. Imperial J Pharmacog Natural Prod 1:145–151

    Google Scholar 

  16. Baskaran N, Jayabalan, (2008) Effect of growth regulators on rapid micropropagation and psoralen production in P. corylifoliaL. Acta Physiologiae Plantarum 30(3):345–351

    Article  CAS  Google Scholar 

  17. Jeyakumar M, Jayabalan N (2002) In vitro plant regeneration from cotyledonary node of P. corylifoliaL. Plant Tissue cult. 12(2):125–129

    Google Scholar 

  18. Wani TA, Pandith SA, Gupta AP, Chandra S, Sharma N, Lattoo SK (2017) Molecular and functional characterization of two isoforms of chalcone synthase and their expression analysis in relation to flavonoid constituents in Grewia asiatica L. PloS one 12:e0179155

    Article  Google Scholar 

  19. Shah SN, Wani TA, Ram B, Koul M, Awasthi P, Rajput DS, Reddy GRS (2016) An efficient protocol for in vitro organogenesis and antioxidant studies in Melia dubia Cav.". Afr J Biotech 15(19):768–775

    Article  CAS  Google Scholar 

  20. Pandey V., Agrawal V. (2009). Bioprospecting of Spilanthes species – Micropropagation and bioassay guided isolation of larvicidal compounds against malaria and filarial vectors [Ph.D. thesis]. Delhi: Department of Botany, University of Delhi.

  21. Sehrawat N, Yadav M, Jaiwal PK (2013) Development of an efficient in vitro regeneration protocol for rapid multiplication and genetic improvement of an important endangered medicinal plant Psoralea corylifolia. Asian journal of plant science and research 3(4):88–94

    CAS  Google Scholar 

  22. Gupta NS, Banerjee M, Acharya K (2014) Influence of hormones and explants towards in vitro callusing and shoot organogenesis in a commercially important medicinal Plant. Int. J. Pharm. Sci. Rev. Res., 29:132–137

    CAS  Google Scholar 

  23. Nitnaware KM, Naik DG, Nikam TD (2011) Thidiazuron-induced shoot organogenesis and production of hepatoprotective lignan phyllanthin and hypophyllanthin in Phyllanthus amarus. Plant Cell Tiss Organ Cult 104:101–110

    Article  CAS  Google Scholar 

  24. Teshome S, Feyissa T (2015) In vitro callus induction and shoot regeneration from leaf explants of Glinus lotoides (L.)-an important medicinal plant American. Journal of Plant Sciences 6:1329–1340

    Article  CAS  Google Scholar 

  25. Jiang QB, Zhang Y, Zhong CL, Zeng B, Bogusz D, Franche C (2012) Establishment of an in vitro plant regeneration protocol for Casuarina cunninghamiana Miq. via indirect organogenesis. New Forests. 43(2):143–154

    Article  Google Scholar 

  26. Dhar U, Joshi M (2005) Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators. Plant Cell Rep 24:195–200

    Article  CAS  Google Scholar 

  27. Mohd Anis and Mohd Faisal (2005) In vitro regeneration and mass multiplication of Psoralea corylifolia-an endangered medicinal plant. Indian Journal of Biotechnology 4:261–264

    Google Scholar 

  28. Khaleghi A, Khalighi A, Sahraroo A, Karimi M, Rasoulnia A, Ghafoori IN, Ataei R (2008) In vitro propagation of alstroemeria cv ‘Fuego.’ Am-Eur. J. Agric. Environ. Sci. 3(3):492–497

    Google Scholar 

  29. Mishra K, Ojha H, Chaudhury NK (2012) Estimation of antiradical properties of antioxidants using DPPH assay: a critical review and results. Food Chem 130:1036–1043

    Article  CAS  Google Scholar 

  30. Amit T, Bhakuni RS (2010) New constiuents from Psoralea corylifolia. Ind J Chem 49B(2):256–259

    Google Scholar 

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Acknowledgements

NGN is thankful to the MPCST Bhopal for providing financial assistance and Director Indian institute of integrative medicine (IIIM) CSIR Canal Road Jammu for providing necessary research facilities. TAW is thankful to CSIR for providing Senior Research associateship under Scientist Pool scheme with pool No: 9092A.

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Authors and Affiliations

  1. Department of Botany, Barkattullah University, Bhopal, India

    Nelofar Gulam Nabi

  2. Plant Tissue Culture Lab, Department of Botany, University of Kashmir, Hazratbal, Srinagar, Jammu and Kashmir, 190006, India

    Tareq A. Wani & Zahoor A. Kaloo

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  1. Nelofar Gulam Nabi
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  2. Tareq A. Wani
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  3. Zahoor A. Kaloo
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Contributions

TAW conceived and designed the experiments; NGN performed bulk of the experiments as a part of her PhD programme; Support for chemical analyses was provided by ZAK; Data were analyzed by T.A.W.; ZAK. T.A.W. and NGN wrote the paper. All authors read, reviewed and approved the manuscript.

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Correspondence to Tareq A. Wani.

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Significance statement The present investigation is an efficient, rapid, improved method likely to be used for industrial supply of bulk and quality plant material. It can serve a prospective guide for its future sustainable exploitation.

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Nabi, N.G., Wani, T.A. & Kaloo, Z.A. In Vitro Conservation Strategies for Sustainable Production of Secondary Metabolites in Psoralea corylifolia L.. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 91, 959–970 (2021). https://doi.org/10.1007/s40011-021-01298-z

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  • DOI: https://doi.org/10.1007/s40011-021-01298-z

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