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18S rDNA mapping revealed conservation and rearrangements of chromosome segments in two Channa species

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Snakehead fishes are important food fishes from freshwater ecosystems with their significant contribution in capture fisheries. They also have high potential for aquaculture. Cytogenetic variability between the Channa punctata and C. striata was assessed in the present investigation. The study revealed diploid chromosome number, karyotype configuration and fundamental arm numbers as 32, 18m + 10sm + 4st and 64, respectively, in C. punctata and 40, 6m + 2sm + 10st + 22t and 68 in C. striata without any heteromorphic pair(s) in both the species. Rhodamine-labeled 18S rDNA probe was constructed and hybridized on the metaphase chromosome spreads of both the species. The signals of the hybridized probe were observed on two sub-metacentric and two sub-telocentric chromosome pairs in C. punctata and C. striata, respectively. The study revealed that 18S ribosomal genes are present on morphologically different chromosome pairs which have evolved by means of chromosome fragmentation and their rearrangements during the evolution of the species. The information is useful for 18S gene-bearing chromosomes identification, in cytotaxonomy and documentation of the hybrids of the two species.

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Acknowledgements

The authors are highly thankful to the DBT, New Delhi, for financial support to conduct the experiment and to the Director, ICAR-National Bureau of Fish Genetic Resources, Lucknow, for providing the laboratory facilities and other supports.

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Authors and Affiliations

  1. Molecular Biology and Biotechnology Division, ICAR- National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, India

    Basdeo Kushwaha, Vishwamitra Singh Baisvar & Ravindra Kumar

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  1. Basdeo Kushwaha
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  2. Vishwamitra Singh Baisvar
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  3. Ravindra Kumar
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Correspondence to Ravindra Kumar.

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Conflict of interest

The authors declare no conflict of interest related to this article. Further, both Basdeo Kushwaha and Vishwamitra Singh Baisvar may be considered as the first author in the manuscript due to their equal contributions.

Ethical Approval

For the present work, healthy live specimens of C. punctata and C. striata were collected from Gomti river near Lucknow, Uttar Pradesh, INDIA. All applicable national and institutional guidelines for the care and use of fishes were followed. The undertaken species is not listed in the endangered/ special category or otherwise enlisted fish species and does not restrict the use of this species for laboratory experiments/ research. The experiments with live fish do not come under the CPCSEA (Committee for the Purpose of Control and Supervision on Experiments on Animals) guidelines of the Ministry of Environment, Forest & Climate Change (MoEFCC), Government of India, New Delhi. There are no guidelines available for using live fish for experiments in India as on today.

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Significance Statement: Channa punctata and C. striata are important food fishes and potential aquaculture species. The molecular cytogenetic information revealing the position of 18S rDNA through FISH will be useful in their cytotaxonomy, conservation and management.

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Kushwaha, B., Baisvar, V.S. & Kumar, R. 18S rDNA mapping revealed conservation and rearrangements of chromosome segments in two Channa species. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 91, 675–679 (2021). https://doi.org/10.1007/s40011-021-01257-8

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  • DOI: https://doi.org/10.1007/s40011-021-01257-8

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