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Molecular characterization of eight Indian Snakehead species (Pisces: Perciformes Channidae) using RAPD markers

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Abstract

Murrels (Perciformes; Channidei; Channidae) are unique group of freshwater air breathing fishes having a confined distribution to African and Asian continents. The phylogenetic relationship among eight Channid species viz. Channa aurantimaculata, Channa bleheri, Channa diplogramma, Channa gachua, Channa marulius, Channa punctatus, Channa stewartii and Channa striatus were investigated using RAPD markers. Eight random oligodecamers viz. OPAC03, OPAC05, OPAC07, OPAC09, OPAC19, OPA10, OPA11 and OPA16 were used to generate the RAPD profile. Estimates of Nei’s (Genetics, 89:583–590, 1978) unbiased genetic distance (D) demonstrated sufficient genetic divergence to discriminate the samples of different species and the values ranged from 0.3292 to 0.800 The present RAPD analyses strongly substantiate the view of earlier morphological and osteological studies of Channid species, the closer association among species in “gachua” and “marulius” groups.

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References

  1. Xia Li, Musikasinthorn P, Kumazawa Y (2006) Molecular phylogenetic analyses of Snakeheads Perciformes: Channidae) using mitochondrial DNA sequences. Ichthyol Res 53:148–159

    Article  Google Scholar 

  2. Musikasinthorn P (1998) Channa panaw, a new channid fish from the Irrawaddy and Sittang River basins, Myanmar. Ichthyol Res 45:355–362

    Article  Google Scholar 

  3. Musikasinthorn P (2003) Channoidei (Snakeheads) In: Hutchins M, Thoney A, Loiselle PV, Schlager N (eds) Grzimek’s animal life encyclopedia, 2nd edn. vols 4, 5. Fishes I–II. Gale Group, Farmington Hills, pp 437–447

  4. Musikasinthorn P (2000) Channa aurantimaculata, a new channid fish from Assam (Brahmaputra River basin), India, with designation of a neotype for C. amphibeus (McClelland, 1845). Ichthyol Res 47:27–37

    Article  Google Scholar 

  5. Berra TM (2001) Freshwater fish distribution. Academic Press, San Diego

    Google Scholar 

  6. Musikasinthorn P, Taki Y (2001) Channa siamensis (Günther, 1861), a junior synonym of Channa lucius (Cuvier in Cuvier and Valenciennes, 1831). Ichthyol Res 48:319–324

    Article  Google Scholar 

  7. Zhang C-G, Musikasinthorn P, Watanabe K (2002) Channa nox, a new channid fish lacking a pelvic fin from Guangxi, China. Ichthyol Res 49:140–146

    Article  Google Scholar 

  8. Courtenay WR, Williams JD (2004) Snakeheads (Pisces, Channidae): a biological synopsis and risk assessment. US Geological Survey Circular 1251, p 143

    Google Scholar 

  9. Bonou CA, Teugels GG (1985) Révision systématique du genre Parachanna Teugels et Daget, 1984 (Pisces: Channidae). Rev Hydrobiol Trop 18:267–280

    Google Scholar 

  10. Teugels GG (1992) Channidae. In: Leveque C, Paugy D, Teugels GG (eds) The fresh and brackish water fishes of west Africa, vol 2. ORSTOM et MRAC, Paris, pp 655–658

    Google Scholar 

  11. Vishwanath W, Kh Geethakumari (2009) Diagnosis and interrelationships of fishes of the genus Channa Scopoli (Teleostei: Channidae) of northern. Ind J Threatened Taxa 1(2):97–105

    Google Scholar 

  12. Nagarajan M, Haniffa MA, Gopalakrishnan A, Basheer VS, Muneer Abdul (2006) Gnetic variability of Channa punctatus populations using randomly polymorphic DNA. Aquacult Res 37:1151–1155

    Article  CAS  Google Scholar 

  13. Çiftci Y, Okumus I (2002) Fish population genetics and applications of molecular markers to fisheries and aquaculture: I- basic principles of fish population genetics. Turk J Fish Aquat Sci 2:145–155

    Google Scholar 

  14. Okumus I, Çiftci Y (2003) Fish population genetics and molecular markers: II- molecular markers and their applications in fisheries and aquaculture. Turk J Fish Aquat Sci 3:51–79

    Google Scholar 

  15. Povh AJ, Lopera-Barrero NM, Lupchinski E Jr, Gomes PC, Lopes TS (2008) Genetic monitoring of fish repopulation programs using molecular markers. Cien Inv Agr 35(1):1–10

    Google Scholar 

  16. Welsh J, McClelland M (1990) Fingerprinting genome using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218

    Article  PubMed  CAS  Google Scholar 

  17. Gil LA (2007) PCR-based methods for fish and fishery products authentication. Trends Food Sci Technol 18(11):558–566

    Article  Google Scholar 

  18. Bardakci F (2001) Random amplified polymorphic DNA (RAPD) Markers. Turk J Biol 25:185–196

    CAS  Google Scholar 

  19. Ali BA, Huang T-H, Qin D-N, Wang X-M (2005) A review of random amplified polymorphic DNA (RAPD) markers in fish research. Rev Fish Biol Fish 14:443–453

    Article  Google Scholar 

  20. Ruzzante DE, Taggart CT, Cook C, Goddard S (1996) Genetic differentiation between inshore and offshore Atlantic cod (Gadus morhua) off Newfoundland: microsatellite DNA variation and antifreeze level. Can J Fish Aquat Sci 53:634–645

    Article  Google Scholar 

  21. Archana Saini, Anish Dua, Vindhya Mohindra, Lakra WS (2010) Molecular discrimination of six species of Bagrid catfishes from Indus river system using randomly amplified polymorphic DNA markers. Mol Biol Rep. doi:10.1007/s11033-9960-1

  22. Yeh FC, Yang RC, Boyle T (1999) POPGENE 32- Version 1.31. Population genetics software. http://www.ualberta.ca/ ~fyeh/fyeh

  23. Welsh J, McClelland M (1994) Fingerprinting using arbitrarily primed PCR: applications to genetic mapping, population biology, epidemiology and detection of differentially expressed RNAs. In: Mullis KB, Ferre F, Gibbs RA (eds) The polymerase chain reaction, Brikhauser, Boston, pp 295–303

  24. Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Department of Science and Technology New Delhi (SR/SO/AS-76/2007) for their financial support to carry out this work. We thank Dr. W. Vishvanath, Professor, Dept. of Life Sciences, Manipur University, Manipur India for his permission to use the Fig. 8 which highlight the Ventral view of Channa marulius and Channa gachua.

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Authors and Affiliations

  1. Centre for Aquaculture Research and Extension, St. Xavier’s College (Autonomous), Palayamkottai, Tamilnadu, 627 002, India

    Ajaz Ali Bhat, M. A. Haniffa, M. James Milton & Bilal Ahmad Paray

  2. National Bureau of Fish Genetic Resources (NBFGR) Cochin Unit, CMFRI Campus, Kochi, 682 018, India

    P. R. Divya, A. Gopalakrishnan & Raj Kumar

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  1. Ajaz Ali Bhat
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  2. M. A. Haniffa
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  3. P. R. Divya
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  4. A. Gopalakrishnan
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  5. M. James Milton
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  6. Raj Kumar
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  7. Bilal Ahmad Paray
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Correspondence to M. A. Haniffa.

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Bhat, A.A., Haniffa, M.A., Divya, P.R. et al. Molecular characterization of eight Indian Snakehead species (Pisces: Perciformes Channidae) using RAPD markers. Mol Biol Rep 39, 4267–4273 (2012). https://doi.org/10.1007/s11033-011-1213-4

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  • DOI: https://doi.org/10.1007/s11033-011-1213-4

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