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Statistical Optimization of Bioprocess Parameters for Improved Production of L-Asparaginase from Lactobacillus plantarum

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

L-asparaginase (ASNase), a tetrameric enzyme, holds comprehensive applications in food industries as a reducing agent and pharma industries as an anticancer agent. In this study, we aimed to screen ASNase-producing lactic acid bacteria from cow milk. The statistically designed experiment, Plackett–Burmann design, was employed to screen the most significant media components (L-asparagine, pH and temperature). The Box–Behnken design was employed to optimize the screened parameters to enhance the yield of ASNase. Under the optimized conditions viz. 4.8 g/L of L-asparagine, 41.8 °C and pH 7.02, the enzyme yield was found to be 113.3 U/mL/min. The partially purified ASNase showed enzyme activity of 135.8 U/mL/min with molecular weight of 34 kDa. Further, based on the phenotypic and genotypic characteristics, the potent ASNase-producing strain was identified as Lactobacillus plantarum. This study concludes that the cow milk isolate, L. plantarum, could be a potent candidate for producing extracellular ASNase for further applications in food and pharma industrial sectors.

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Acknowledgements

SM and AR acknowledge Vivekananda College of Arts & Science for Women, SD and AK acknowledge UGC Women PDF, RUSA, 2.0- Biological Sciences, BDU and DST-PURSE, BDU for providing laboratory facilities and financial support.

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Authors and Affiliations

  1. Department of Microbiology, Vivekanandha College of Arts and Science for Women (Autonomous), Tiruchengode, Namakkal, Tamil Nadu, 637 303, India

    Sivasankari Mathiyalagan

  2. Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India

    Senbagam Duraisamy & Anbarasu Kumarasamy

  3. Department of Medical Microbiology, College of Health and Medical Sciences, Haramaya University, Harar Campus, P.O. Box 235, Harar, Ethiopia

    Senthilkumar Balakrishnan

  4. Department of Biotechnology, Periyar University, Salem, Tamil Nadu, 636 011, India

    Amutha Raju

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  1. Sivasankari Mathiyalagan
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Significance Statement This study emphasized on the statistical optimization of bioprocess parameters for the enhanced asparaginase production by Lactobacillus plantarum isolate.

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Mathiyalagan, S., Duraisamy, S., Balakrishnan, S. et al. Statistical Optimization of Bioprocess Parameters for Improved Production of L-Asparaginase from Lactobacillus plantarum. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 91, 441–453 (2021). https://doi.org/10.1007/s40011-021-01234-1

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  • DOI: https://doi.org/10.1007/s40011-021-01234-1

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