Abstract
CXCR1 (Interleukin-8 Receptor A) is one of the two high-affinity receptors (IL-8RA and IL-8RB), present on the surface of neutrophils that binds to the IL-8 ligand, which results in the activation of immune response and allows the migration of immune cells to the site of inflammation. CXCR1 is strongly associated with inflammatory responses against most of the Gram-negative bacteria. The objective of the present work was to amplify, sequence and in silico characterize CXCR1 coding region of buffalo. The amplified target gene of buffalo CXCR1 was custom-sequenced, and the annotated sequence of 1130 bp having coding region of 1083 bp was submitted to DDBJ with accession number LC384988. Pairwise sequence alignment of buffalo CXCR1 and the reference cattle CXCR1 showed 95% similarity. Sequence analysis and integrative analysis of CXCR1 in 36 divergent species revealed that 7 codon positions have undergone positive selection pressure, indicating that the gene has undergone evolutionary changes over the period of time. Evolutionary analysis of the CXCR1 (MEGA 6 and Datamonkey online server) revealed that ruminant, avian, fish CXCR1 formed different clusters within the phylogenetic tree, showing evolutionary divergence.
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References
Beecher C, Daly M, Childs S, Berry DP, Magee DA, McCarthy TV, Giblin L (2010) Polymorphisms in bovine immune genes and their associations with somatic cell count and milk production in dairy cattle. BMC Genet 11(1):99–102
Abera M, Demie B, Aragaw K, Regassa F, Regassa A (2010) Isolation and identification of Staphylococcus aureus from bovine mastitic milk and their drug resistance patterns in Adama Town, Ethiopia. J Vet Med Anim Health 2(3):29–34
Chuntharapai A, Lee J, Hebert CA, Kim KJ (1994) Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes. J Immunol 153(12):5682–5688
Burvenich C, Paape MJ, Hill AW, Guidry AJ, Miller RH, Heyneman R, Brand A (1994) Role of the neutrophil leucocyte in the local and systemic reactions during experimentally induced E. coli mastitis in cows immediately after calving. Vet Q 16(1):45–50
Oviedo-Boyso J, Valdez-Alarcón JJ, Cajero-Juárez M, Ochoa-Zarzosa A, López-Meza JE, Bravo-Patino A, Baizabal-Aguirre VM (2007) Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis. J Infect 54(4):399–409
Rainard P, Riollet C (2006) Innate immunity of the bovine mammary gland. Vet Res 37(3):369–400
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215(3):403–410
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35(6):1547–1549
Hasegawa M, Kishino H, Yano T (1985) Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 22(2):160–174. https://doi.org/10.1007/bf02101694
Tamura K, Kumar S (2002) Evolutionary distance estimation under heterogeneous substitution pattern among lineages. Mol Biol Evol 19(10):1727–1736
Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the eighbour-joining method. Proc Natl Acad Sci USA 101(30):11030–11035
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729
Pond SLK, Frost SD (2005) Datamonkey: rapid detection of selective pressure on individual sites of codon alignments. Bioinformatics 21(10):2531–2533
Chen C, Li Z, Zhou Z, Yin Z, Chan SM, Yu XQ, He J (2009) Cloning, characterization and expression analysis of a CXCR1-like gene from mandarin fish Siniperca chuatsi. Fish Physiol Biochem 35(3):489–499
Dige MS, Ahlawat SPS, Bhushan B, Kumar A, Kumar P, Dhama K, Bhatt P (2015) Molecular characterization of Exon-2 of CXCR2 Gene in Vrindavani Cattle. Vet Pract 16(2):291–293
Hedges SB (1992) The number of replications needed for accurate estimation of bootstrap P value in Phylogenetic studies. Proc R Soc 276:4261–4270
Widdison S, Siddiqui N, Easton V, Lawrence F, Ashley G, Werling D, Watson M, Coffey TJ (2010) The bovine chemokine receptors and their mRNA abundance in mononuclear phagocytes. BMC Genom 11:439
Liu Y, Yang S, Lin AA, Cavalli-Sforza LL, Su B (2005) Molecular evolution of CXCR1, a G protein-coupled receptor involved in signal transduction of neutrophils. J Mol Evol 61(5):691–696
Dhaliwal KK, Arora JS, Mukhopadhyay CS, Dubey PP (2015) In silico characterization of functional divergence of two cathelicidin variants in Indian sheep. Evolut Bioinform Online 11:189
Xu T, Zhu Z, Sun Y, Ren L, Wang R (2014) Characterization and expression of the CXCR1 and CXCR4 in miiuy croaker and evolutionary analysis shows the strong positive selection pressures imposed in mammal CXCR1. Dev Comp Immunol 44(1):133–144
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Financial assistance for conducting the research work provided by UGC and RKVY is highly acknowledged.
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Significance Statement
This is the first report related to the sequencing of CXCR1 (IL-8RA) gene in buffalo (Bubalus bubalis). Evolutionary divergence studies have shown the positive selection of CXCR1 gene over the period of time.
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Rajput, S., Arora, J.S., Mukhopadhyay, C.S. et al. Evolutionary Divergence of CXCR1 (Interleukin-8 Receptor A) Gene of Indian Water Buffalo (Bubalus bubalis) in Light of Molecular Evolution. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 1351–1359 (2019). https://doi.org/10.1007/s40011-018-1058-x
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DOI: https://doi.org/10.1007/s40011-018-1058-x