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Cloning, characterization and expression analysis of a CXCR1-like gene from mandarin fish Siniperca chuatsi

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Abstract

In this study we cloned and characterized a CXCR1-like gene (mfCXCR1) from mandarin fish (Siniperca chuatsi). The full-length cDNA of mfCXCR1 is 2,173 bp and contains a 1,056 bp open reading frame (ORF) that encodes a protein of 351 amino acids. The 5′ and 3′ untranslated regions (UTR) are 57 and 1,080 bp in length, respectively. The coding region of the mfCXCR1 gene consists of a single exon with a 734 bp intron that is two nucleotides upstream of the ATG start codon in the 5′ UTR. The mfCXCR1 protein shares a relatively high identity with the CXCR1 and CXCR2 proteins of other fishes (~50–65%). Furthermore, phylogenetic analysis indicates a close relatedness of mfCXCR1 to CXCR1 of other fishes. Many binding sites for stress-inducible transcription factors were present in the promoter region of the mfCXCR1 gene, indicating that it might be activated by certain stressors. The level of mfCXCR1 mRNA, when normalized to that in liver (1-fold), was highest in spleen (~192.9-fold), with intermediate levels in kidney (~163.2-fold), blood (~131.2-fold) and head kidney (~109.4-fold), and relatively low levels in intestine (~34.4-fold) and gill (~16.4-fold) (P < 0.05). Expression of mfCXCR1 during the clinical stage of infectious spleen and kidney necrosis virus (ISKNV) infection showed that its expression was regulated over the course of infection. On day 4 after ISKNV challenge, mfCXCR1 expression was down-regulated in blood (~0.91-fold), spleen (~0.26-fold), head kidney (~0.18-fold) and kidney (~0.82-fold).

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Acknowledgements

This research was supported by the Chinese National ‘863’ Project (grant no. 2003AA603011), National Natural Science Foundation of China (grant nos. 30300266, 30325035) and funds from the Science and Technology Bureau of Guangdong Province.

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Authors and Affiliations

  1. State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University, 135 Xingang Road West, Guangzhou, 510275, People’s Republic of China

    Chao Chen, Zhongsheng Li, Zhicheng Zhou, Zhixin Yin, Shaoping Weng & Jianguo He

  2. Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, People’s Republic of China

    Siu-Ming Chan

  3. Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO, 64110, USA

    Xiao-Qiang Yu

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  1. Chao Chen
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Correspondence to Jianguo He.

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Chen, C., Li, Z., Zhou, Z. et al. Cloning, characterization and expression analysis of a CXCR1-like gene from mandarin fish Siniperca chuatsi . Fish Physiol Biochem 35, 489–499 (2009). https://doi.org/10.1007/s10695-008-9283-5

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