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Cytochrome-C Oxidase I Gene-Based Genetic Divergence and Molecular Phylogeny Among the Species of Fish Genus Channa

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The freshwater snakehead fishes are members of the family Channidae and order Perciform, which are native to parts of Africa and Asia with widespread distributions. The present study was aimed to assess the genetic variability and molecular phylogeny among 22 species of genus Channa, viz. C. argus, C. asiatica, C. aurantimaculata, C. bankanensis, C. barca, C. bleheri, C. burmanica, C. diplogramma, C. gachua, C. lucius, C. maculata, C. marulius, C. melasoma, C. micropeltis, C. orientalis, C. ornatipinnis, C. panaw, C. pleurophthlama, C. pulchra, C. punctata, C. stewartii and C. striata, using partial 513 bp long consensus sequence of cytochrome-c oxidase subunit I mitochondrial DNA retrieved from NCBI (March, 2018). The study showed 281 haplotypes with 267 variable sites (i.e., 33 singletons and 234 parsimoniously informative) in nucleotide positions. Most of the sequences were A + T rich, except in C. argus which is G + C rich (50.25%). The NJ and MP trees showed similar topology with discrete branches, each as a distinct species of the Channa with slight differences in bootstrap values. Maximum K2P distance was found between the C. ornatipinnis and the C. melasoma. The maximum numbers of polymorphic loci were present in C. gachua.

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Acknowledgements

The authors gratefully thank to ICAR-National Bureau of Fish Genetic Resources, Lucknow for providing the financial support and encouragement. The financial support for tissue sampling and DNA sequencing of fishes was taken under the institutional projects entitled ‘Genetic stock structure elucidation of Tenualosa ilisha and Channa striata using microsatellite, mitochondrial DNA and molecular cytogenetic markers’ and ‘DNA barcoding of India fishes,’ which is duly acknowledged.

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Authors and Affiliations

  1. Molecular Biology and Biotechnology Division, ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, Uttar Pradesh, 226 002, India

    Vishwamitra Singh Baisvar, Ravindra Kumar, Mahender Singh & Basdeo Kushwaha

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  1. Vishwamitra Singh Baisvar
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  2. Ravindra Kumar
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  3. Mahender Singh
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  4. Basdeo Kushwaha
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Correspondence to Ravindra Kumar.

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Significance Statement

Genetic variability among 22 Channa spp. was assessed using COI gene. Study showed 281 haplotypes with 267 variable sites. C. gachua had maximum polymorphic loci. Maximum genetic distance using K2P was found between C. ornatipinnis and C. melasoma.

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Baisvar, V.S., Kumar, R., Singh, M. et al. Cytochrome-C Oxidase I Gene-Based Genetic Divergence and Molecular Phylogeny Among the Species of Fish Genus Channa. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 1455–1463 (2019). https://doi.org/10.1007/s40011-018-01070-w

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