Skip to main content
SpringerLink
Log in

Trichoderma harzianum ThU and Its Metabolites Underscore Alteration in Essential Oils of Ocimum basilicum and Ocimum sanctum

  • Research Article
  • Published:
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Ocimum, an important commercial aromatic crop, is well known for the industrially acclaimed essential oil. In the present study, a comparative analysis was performed between Trichoderma harzianum (ThU) and its secondary metabolites on plant growth, essential oil and phytochemical parameters in O. basilicum and O. sanctum. A significant enhancement in biomass and essential oil content (2.37 and 2.16 fold, respectively) was observed in the live culture of ThU (TH_CC) treatment of O. basilicum, whereas its metabolites (TH_CF) enhanced biomass (1.25 folds) and essential oil (2.17 folds) in O. sanctum as compared to their respective controls. The present study first time highlights species dependent response in plants by the application of TH_CC and TH_CF to enhance plant growth, antioxidants and oil content of Ocimum. The present results thus provide a better understanding of the host specific response to ThU and its metabolites which can be used to develop effective biofertilizers for crop improvement.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Gupta R, Pandey R (2015) Microbial interference ameliorates essential oil yield and diminishes root-knot infestation in sweet basil under field conditions. Biocontrol Sci Technol 25:1165–1179

    Article  Google Scholar 

  2. Sajjadi SE (2006) Analysis of the essential oils of two cultivated basil (Ocimum basilicum L.) from Iran. DARU J Pharm Sci 14:128–130

    CAS  Google Scholar 

  3. Hussain AI, Anwar F, Sherazi STH, Przybylski R (2008) Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depend on seasonal variations. Food Chem 108:986–995

    Article  CAS  PubMed  Google Scholar 

  4. Saharan BS, Verma S (2015) Evaluation of rhizospheric bacteria from Ocimum sp. as potential PGPR. J Microb Biochem Technol 7:088–095

    Article  Google Scholar 

  5. Gupta R, Tiwari S, Saikia SK, Shukla V, Singh R, Singh SP, Ajay Kumar PV, Pandey R (2015) Exploitation of microbes for enhancing bacoside content and reduction of Meloidogyne incognita infestation in Bacopa monnieri L. Protoplasma 252:53–61

    Article  CAS  PubMed  Google Scholar 

  6. Vinale F, Sivasithamparam K, Ghisalberti EL, Ruocco M, Wood S, Lorito M (2012) Trichoderma secondary metabolites that affect plant metabolism. Nat Prod Commun 7:1545–1550

    CAS  PubMed  Google Scholar 

  7. Compant S, Clément C, Sessitsch A (2010) Plant growth-promoting bacteria in the rhizo-and endosphere of plants: their role, colonization, mechanisms involved and prospects for utilization. Soil Biol Biochem 42:669–678

    Article  CAS  Google Scholar 

  8. Arnon DI (1949) Copper enzymes in isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592

    Article  CAS  Google Scholar 

  10. Yemm EW, Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508–514

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158

    CAS  Google Scholar 

  12. Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

    Article  CAS  Google Scholar 

  13. Brand-Williams W, Cuvelier ME, Berset CLWT (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30

    Article  CAS  Google Scholar 

  14. Zheng W, Wang SY (2001) Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 49:5165–5170

    Article  CAS  PubMed  Google Scholar 

  15. Gupta R, Saikia SK, Pandey R (2015) Bioconsortia augments antioxidant and yield in Matricaria recutita L. against Meloidogyne incognita (Kofoid and White) Chitwood infestation. Proc Nat Acad Sci Ind Sec B Biol. doi:10.1007/s40011-015-0621-y

    Google Scholar 

  16. Pandey R, Mishra AK, Tiwari S, Singh HN, Kalra A (2011) Enhanced tolerance of Mentha arvensis against Meloidogyne incognita (Kofoid and White) Chitwood through mutualistic endophytes and PGPRs. J Plant Interact 6:247–253

    Article  Google Scholar 

  17. Nigro M, Vinale F, Ruocco M, Woo SL, Lanzuise S, Marra R, Eid A, Lorito M (2012) Role of Trichoderma secondary metabolites in plant interactions: the metabolic changes in Arabidopsis thaliana. J Plant Pathol 94:71

    Google Scholar 

  18. Yadava S, Sarangi PK, Das M (2014) Ecotechnology of compost from biodegradables inoculated by Trichoderma and its effects on growth of medicinal plant Ocimum gratissimum L. Ann Plant Sci 3:610–613

    Google Scholar 

  19. Vafadar F, Amooaghaie R, Otroshy M (2014) Effects of plant-growth-promoting rhizobacteria and arbuscular mycorrhizal fungus on plant growth, stevioside, NPK, and chlorophyll content of Stevia rebaudiana. J Plant Interact 9:128–136

    Article  CAS  Google Scholar 

  20. Gupta R, Singh A, Pandey R (2016) Microbes based technology ameliorates glandular trichomes, secondary metabolites and antioxidant in Pelargonium graveolens L’Her. J Sci Food Agric. doi:10.1002/jsfa.7617

    Google Scholar 

  21. Keswani C, Mishra S, Sarma BK, Singh SP, Singh HB (2014) Unraveling the efficient applications of secondary metabolites of various Trichoderma spp. Appl Microbiol Biotechnol 98:533–544

    Article  CAS  PubMed  Google Scholar 

  22. Mukherjee PK, Horwitz BA, Kenerley CM (2012) Secondary metabolism in Trichoderma—a genomic perspective. Microbiol 158:35–45

    Article  CAS  Google Scholar 

  23. Singh A, Jain A, Sarma BK, Upadhyay RS, Singh HB (2014) Beneficial compatible microbes enhance antioxidants in chickpea edible parts through synergistic interactions. LWT Food Sci Technol 56:390–397

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was funded by CSIR-Twelfth Five Year Plan Project (BSC0203). The authors are thankful to the Director, CSIR-Central Institute of Medicinal and Aromatic Plants Lucknow, India for providing necessary facilities. Help extended by Dr. C. S Chanotiya and Ms. Anju Yadav in GC analysis is greatly appreciated.

Author information

Authors and Affiliations

  1. Department of Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, 226015, India

    Rupali Gupta, Akanksha Singh, Ranjana Kanaujia, Shaivya Kushwaha & Rakesh Pandey

Authors
  1. Rupali Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akanksha Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ranjana Kanaujia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shaivya Kushwaha
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rakesh Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rakesh Pandey.

Ethics declarations

Conflict of interest

The authors declare that there exists no potential conflict of interest among them.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 2522 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gupta, R., Singh, A., Kanaujia, R. et al. Trichoderma harzianum ThU and Its Metabolites Underscore Alteration in Essential Oils of Ocimum basilicum and Ocimum sanctum . Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 219–227 (2018). https://doi.org/10.1007/s40011-016-0753-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40011-016-0753-8

Keywords

Search

Navigation