Skip to main content
SpringerLink
Log in

Genetic Similarity Using MLST Amongst Campylobacter jejuni Isolates from Children with Diarrhea Symptoms and Broilers

  • Research Article
  • Published:
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The aim of the present study was to investigate the genetic similarity amongst Campylobacter jejuni isolates from children with diarrhea symptoms and broilers in Chiang Mai, Thailand during 2002–2007. Sequence types of 12 isolates from children with symptoms of diarrhea and 9 isolates from broilers and other 42 Thai sequence types were determined by multilocus sequence typing. Cluster analysis was performed to determine the molecular relatedness of the isolates. Results showed diverse sequence types of C. jejuni isolated from both broilers and children. One isolate each from a broiler and a child shared a single sequence type (574). The majority of 13/16 sequence types belonged to previously reported sequence types in Thailand and two previously reported human sequence types were identified in broilers. The authors conclude that there is evidence of a genetic relationship between C. jejuni isolated from humans and broilers. MLST analysis of C. jejuni suggested that broilers are one of the sources of human infection in Chiang Mai, Thailand.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Bodhidatta L, Vithayasai N, Eimpokalarp B, Pitarangsi C, Serichantalergs O, Isenbarger D (2002) Bacterial enteric pathogens in children with acute dysentery in Thailand: increasing importance of fluoroquinolone-resistant Campylobacter. Southeast Asian J Trop Med Public Health 33:752–757

    CAS  PubMed  Google Scholar 

  2. Padungtod P, Kaneene JB (2005) Campylobacter in food animals and humans in northern Thailand. J Food Prot 68:2519–2526

    Article  PubMed  Google Scholar 

  3. Meeyam T, Padungtod P, Kaneene J (2004) Molecular characterization of Campylobacter isolated from chickens and humans in northern Thailand. Southeast Asian J Trop Med Public Health 35:670–675

    PubMed  Google Scholar 

  4. Bull SA, Allen VM, Domingue G, Jorgensen F, Frost JA, Ure R, Whyte R, Tinker D, Corry JEI, Gillard-King J, Humphrey TJ (2006) Source of Campylobacter spp. colonizing housed broiler flocks during rearing. App Environ Microbiol 72:645–652

    Article  CAS  Google Scholar 

  5. Sopwith W, Birtles A, Matthew M, Fox A, Gee S, Painter M, Regan M, Syed Q, Bolton E (2006) Campylobacter jejuni multilocus sequence types in humans, Northwest England, 2003–2004. Emerg Infect Dis 12:1500–1507

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Levesque S, Frost E, Arbeit RD, Michaud S (2008) Multilocus sequence typing of Campylobacter jejuni isolates from humans, chickens, raw milk, and environmental water in Quebec, Canada. J Clin Microbiol 46:3404–3411

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Miller WG, On SL, Wang G, Fontanoz S, Lastovica AJ, Mandrell RE (2005) Extended multilocus sequence typing system for Campylobacter coli, C. lari , C. upsaliensis, and C. helveticus. J Clin Microbiol 43:2315–2329

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Behringer M, Miller WG, Oyarzabal OA (2011) Typing of Campylobacter jejuni and Campylobacter coli isolated from live broilers and retail broiler meat by flaA-RFLP, MLST, PFGE and REP-PCR. J Microbiol Methods 84:194–201

    Article  CAS  PubMed  Google Scholar 

  9. Griekspoor P, Engvall EO, Olsen B, Waldenström J (2010) Multilocus sequence typing of Campylobacter jejuni from broilers. Vet Microbiol 140:180–185

    Article  CAS  PubMed  Google Scholar 

  10. Taboada EN, Clark CG, Sproston EL, Carrillo CD (2013) Current methods for molecular typing of Campylobacter species. J Microbiol Methods 95:24–33

    Article  CAS  PubMed  Google Scholar 

  11. Fearnley C, Manning G, Bagnall M, Javed MA, Wassenaar TM, Newell DG (2008) Identification of hyperinvasive Campylobacter jejuni strains isolated from poultry and human clinical sources. J Med Microbiol 75:570–580

    Article  Google Scholar 

  12. Manfreda G, Parisi A, De Cesare A, Mion D, Piva S, Zanoni RG (2015) Typing of Campylobacter jejuni isolated from turkey by genotypic methods, antimicrobial susceptibility, and virulence gene patterns: a retrospective study. Foodborne Pathog Dis. doi:10.1089/fpd.2015.2048

    PubMed  Google Scholar 

  13. Jolley KA, Maiden MC (2010) BIGSdb: scalable analysis of bacterial genome variation at the population level. BMC Bioinform 11:595

    Article  Google Scholar 

  14. Ausubel F, Brent R, Kingston R, Moore D, Seidman JG, Smith J (1999) Short protocols in molecular biology, 4th edn. Wiley, New York

    Google Scholar 

  15. Dingle KE, Colles FM, Wareing DRA, Ure R, Fox AJ, Bolton FE, Bootsma HJ, Willems RJL, Urwin R, Maiden MCJ (2001) Multilocus sequence typing system for Campylobacter jejuni. J Clin Microbiol 39:14–23

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Pursing DH (2004) Molecular microbiology diagnostic principle and practice, 3rd edn. ASM Press, Washington, pp 235–242

    Google Scholar 

  17. Patchanee P, Chokboonmongkol C, Zessin KH, Alter T, Pornaem S, Chokesajjawatee N (2012) Comparison of multilocus sequence typing (MLST) and repetitive sequence-based PCR (rep-PCR) fingerprinting for differentiation of Campylobacter jejuni isolated from broiler in Chiang Mai, Thailand. J Microbiol Biotechnol 22(11):1467–1470

    Article  CAS  PubMed  Google Scholar 

  18. Chokboonmongkol C, Patchanee P, Golz G, Zessin KH, Alter T (2013) Prevalence, quantitative load, and antimicrobial resistance of Campylobacter spp. from broiler ceca and broiler skin samples in Thailand. Poult Sci 92:462–467

    Article  CAS  PubMed  Google Scholar 

  19. Manning G, Dowson C, Bagnall M, Ahmed I, West M, Newell D (2003) Multilocus sequence typing for comparison of veterinary and human isolates of Campylobacter jejuni. App Environ Microbiol 69:6370–6379

    Article  CAS  Google Scholar 

  20. Mickan L, Doyle R, Valcanis M, Dingle KE, Unicomb L, Lanser J (2006) Multilocus sequence typing of Campylobacter jejuni isolates from New South Wales, Australia. J App Microbiol 102:144–152

    Article  Google Scholar 

  21. Gormley JF, MacRae M, Forbes JK, Ogden DI, Dallas FJ, Strachan JCN (2008) Has retail chicken played a role in the decline of human Campylobacteriosis. App Environ Microbiol 74:383–390

    Article  CAS  Google Scholar 

  22. Colles FM, Jones K, Harding RM, Maiden MCJ (2003) Genetic diversity of Campylobacter jejuni isolates from farm animals and the farm environment. App Environ Microbiol 69:7409–7413

    Article  CAS  Google Scholar 

  23. Nachamkin I, Szymanski CM, Blaser MJ (2008) Campylobacter, 3rd edn. ASM Press, Washington, pp 28–36

    Google Scholar 

Download references

Acknowledgments

It is a pleasure to thank all the supporters, the Thailand Research Fund (TRF) for financial support and Faculty of Veterinary Medicine, Chiang Mai University under TRF-master research grants 2008 (TRF-MRG-WII515S074). This publication made use of the Campylobacter Multi Locus Sequence Typing website (http://pubmlst.org/campylobacter/) developed by Keith Jolley and sited at the University of Oxford [13]. The development of this site has been funded by the Wellcome Trust.

Author information

Authors and Affiliations

  1. Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand

    Bhunikar Wongbundit, Pawin Padungtod, Kannika Na Lampang, Takuo Sawada & Nattawooti Sthitmatee

  2. Laboratory of Veterinary Microbiology, Nippon Veterinary and Life Science University, Tokyo, 180-8602, Japan

    Takuo Sawada

Authors
  1. Bhunikar Wongbundit
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pawin Padungtod
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kannika Na Lampang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takuo Sawada
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nattawooti Sthitmatee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nattawooti Sthitmatee.

Ethics declarations

Conflict of interest

All the authors declare that they have no conflict of interest in the aspect of either financial or personal relationships with other people or organizations that could inappropriately influence their work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wongbundit, B., Padungtod, P., Lampang, K.N. et al. Genetic Similarity Using MLST Amongst Campylobacter jejuni Isolates from Children with Diarrhea Symptoms and Broilers. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 1399–1405 (2017). https://doi.org/10.1007/s40011-016-0720-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40011-016-0720-4

Keywords

Search

Navigation