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Effect of Different Factors During In Vitro Growth and Multiplication in Groundnut Cultivar ‘JL-24’

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

During the present study effect of different factors were studied during in vitro growth and multiplication of adventitious shoot buds induced from de-embryonated cotyledon explants of groundnut cultivar ‘JL-24’. Optimum shoot multiplication rate of 7.0 folds and average shoot length of 3.0 cm was achieved on Murashige and Skoog’s (MS) medium supplemented with 2.0 mgl−1 6-benzylamino purine. During subsequent subculture after every 21 days, an initial shoot number of 3 shoots per explant produced a maximum of 21 shoots per explant after 9 weeks. Sucrose at 3.0 % concentration supported the optimum shoot multiplication rate of 7.0 folds. Lowering or increasing sucrose concentration from the control (3.0 %) proved detrimental for in vitro shoot multiplication and growth. In case of liquid MS medium (without agar) supplemented with 6-benzylamino purine (2.0 mgl−1) and sucrose (3.0 %) the multiplication rate was reduced by 1.5 folds (after 6 weeks) as compared to the semi-solid medium (with 0.8 % agar). Hyperhydricity was also observed in liquid medium. Shoots measuring 3.0–4.0 cm obtained from multiplying cultures were found suitable for rooting. Cent per cent rooting was achieved on 1/4th MS medium with sucrose (2.0 %), agar (0.8 %) and indoleacetic acid (0.5 mgl−1). Subsequent hardening and acclimatization led to 95 % survival of in vitro raised plants.

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Acknowledgments

The present work was supported by Council of Scientific and Industrial Research, Government of India. The author is thankful to CSIR, New Delhi for her research fellowship.

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  1. Department of Botany, Mohanlal Sukhadia University, Udaipur, Rajasthan

    R. Trivedi

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  1. R. Trivedi
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Trivedi, R. Effect of Different Factors During In Vitro Growth and Multiplication in Groundnut Cultivar ‘JL-24’. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 131–137 (2016). https://doi.org/10.1007/s40011-014-0418-4

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