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Effect of Engineered Nanoparticles of Fe and Zn Oxides on Enzyme Activity and Bacterial Abundance in Soil at Ambient and Elevated Atmospheric CO2

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Nanomaterials are finding widespread applications in industry, agriculture and environment due to their unique properties. But, before these materials are widely in use, their impact on soil ecology needs to be thoroughly investigated. In addition, how the nanoparticles behave in soil under elevated CO2 is unknown. A pot culture experiment was conducted under controlled conditions in phytotron to study the effect of nanoparticles on microbial biomass and enzyme activity in soil under rice crop at ambient and elevated atmospheric CO2. Manufactured nanoparticles of Fe and Zn (n-Fe2O3 and n-ZnO) were added to soil at concentrations of 100, 500 and 1,000 mg kg−1. FeSO4 (11.1 mg kg−1), ZnSO4 (5.33 mg kg−1) and ‘control’ treatments were also included for comparison. Results showed highest bacterial population at 100 mg kg−1 n-Fe2O3, which significantly decreased at 500 and 1,000 mg kg−1 n-Fe2O3 or n-ZnO. Similarly the dehydrogenase activity is significantly higher with 100 mg kg−1 n-Fe2O3 as compared to control and FeSO4 or ZnSO4. As compared to 100 mg kg−1 concentration, there was twofold to fivefold decrease in fluorescein diacetate activity at 500 and 1,000 mg kg−1 of nano-Fe2O3 treatment. At elevated CO2, the microbial biomass and activities were higher at tillering than panicle initiation of rice crop over ambient CO2 with 100 mg kg−1 n-Fe2O3 and ZnO than other treatments. It is concluded that n-Fe2O3 and n-ZnO at 100 mg kg−1 is effective to maintain various soil microbiological process but at higher concentrations (e.g., 500 and 1,000 mg kg−1) negative impacts on soil ecology can be expected.

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Acknowledgments

One of the authors, Ramesh Chandra Yadav, acknowledges to the Indian Council of Agricultural Research (ICAR, New Delhi, India) for the grant of Junior Research Fellowship for his Master Degree Programme. The contribution of reviewers and editors is also thankfully acknowledged.

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Authors and Affiliations

  1. Division of Soil Science and Agricultural Chemistry, Indian Agricultural Research Institute, New Delhi, 110012, India

    R. C. Yadav, A. K. Patra & T. J. Purakayastha

  2. National Phytotron Facility, Indian Agricultural Research Institute, New Delhi, 110012, India

    R. Singh

  3. The National Academy of Sciences India, 5 Lajpatrai Road, Allahabad, 211002, India

    C. Kumar

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  1. R. C. Yadav
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  2. A. K. Patra
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  3. T. J. Purakayastha
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Correspondence to A. K. Patra.

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Yadav, R.C., Patra, A.K., Purakayastha, T.J. et al. Effect of Engineered Nanoparticles of Fe and Zn Oxides on Enzyme Activity and Bacterial Abundance in Soil at Ambient and Elevated Atmospheric CO2 . Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 649–656 (2014). https://doi.org/10.1007/s40011-014-0316-9

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  • DOI: https://doi.org/10.1007/s40011-014-0316-9

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