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Haemato-biochemical Responses in Cyprinus carpio (Linnaeus, 1758) Fry Exposed to Sub-lethal Concentration of a Phenylpyrazole Insecticide, Fipronil

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Abstract

The present study evaluates the haemato-biochemical responses associated with fipronil exposure [(±)-5-amino-1-(2,6-dichloro-α,α,α-trifluoro-p-tolyl)-4-trifluoromethylsulfinyl-pyrazole-3-carbonitrile] in Cyprinus carpio fry. Fish were exposed to sublethal concentration (1/3rd of LC50) (0.142 mg L−1) for 15 days and corresponding changes in different haemato-biochemical parameters were recorded at the end of experimental period. Significant (P < 0.05) increase in white blood cell counts, blood glucose, serum complement reactive protein and serum cortisol level were noticed, whereas haemoglobin and serum total protein contents were significantly (P < 0.05) decreased. Aspartate amino transferase, catalase and super oxide dismutase activities were significantly (P < 0.05) increased while alkaline phosphatase and malate dehydrogenase activities were significantly (P < 0.05) decreased. Similarly, 47 % inhibition in acetylcholine esterase activity was noticed due to fipronil stress. Results indicated that sublethal exposure of fipronil can induce haemato-biochemical alterations causing stress to C. carpio fry. Thus, haemato-biochemical parameters can be used as biomarkers for the sublethal toxicity of fipronil in the water bodies.

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Acknowledgments

The financial support and necessary facilities provided by Indian Council of Agricultural Research (ICAR), New Delhi to first author is duly acknowledged. Experimental animals were conducted in accordance with national and institutional guidelines for the protection of animal welfare.

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Authors and Affiliations

  1. Division of Aquaculture, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    S. K. Gupta, N. Saharan & Chandra Prakash

  2. Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    A. K. Pal, N. P. Sahu, M. S. Akhtar & Sikendra Kumar

  3. Directorate of Coldwater Fisheries Research, Chhirapani Fish Farm, Champawat, 262523, Uttrakhand, India

    S. K. Gupta

  4. Directorate of Coldwater Fisheries Research, Bhimtal, 263136, Uttrakhand, India

    M. S. Akhtar

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  1. S. K. Gupta
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Gupta, S.K., Pal, A.K., Sahu, N.P. et al. Haemato-biochemical Responses in Cyprinus carpio (Linnaeus, 1758) Fry Exposed to Sub-lethal Concentration of a Phenylpyrazole Insecticide, Fipronil. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 113–122 (2014). https://doi.org/10.1007/s40011-013-0201-y

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