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Organization and Classification of Cytochrome P450 Genes in Castor (Ricinus communis L.)

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Abstract

Castor is an important non-edible oilseed crop with several industrial applications. Cytochrome P450s represent ~1 % of plant proteome and constitutes one of the largest family of enzymes controlling primary and secondary metabolism. Analysis of castor genomic resources identified 210 putative Cytochrome P450 genes. Based on sequence similarity with Arabidopsis orthologs and CYP nomenclature these genes have been classified into 45 families representing 77 subfamilies and grouped into ten clans. Genes pertaining to ten CYP families (CYP80, CYP92, CYP702, CYP705, CYP708, CYP728, CYP729, CYP733, CYP736 and CYP749) are not present in the castor genome. Maximum number (92) of CYP450 genes possessed single intron followed by intron less genes(35),two intron containing genes (25) and four intron containing genes (20). Deduced CYP proteins of castor on an average exhibited 485 amino acid residues. In general, among the subfamily members conserved sequences as well as length of exons and phasing of introns have been observed. However, variable intron length(s) recorded was attributed to continuous genome expansion. Distinctive phylogenetic groups of castor CYPs showed varying levels of conserved gene organization. A novel gene RcCYPN could be identified in the present study.

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Acknowledgments

The authors thank to Prof. T. Papi Reddy former Head, Department of Genetics, Osmania University for the critical evaluation of the manuscript.

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Authors and Affiliations

  1. Centre for Plant Molecular Biology, Osmania University, Hyderabad, 500007, India

    Maryada Shailendar Kumar, Peram Ravindra Babu, Khareedu Venkateswara Rao & Vudem Dashavantha Reddy

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  1. Maryada Shailendar Kumar
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  2. Peram Ravindra Babu
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  3. Khareedu Venkateswara Rao
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  4. Vudem Dashavantha Reddy
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Correspondence to Vudem Dashavantha Reddy.

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Kumar, M.S., Babu, P.R., Rao, K.V. et al. Organization and Classification of Cytochrome P450 Genes in Castor (Ricinus communis L.). Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 131–143 (2014). https://doi.org/10.1007/s40011-013-0192-8

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  • DOI: https://doi.org/10.1007/s40011-013-0192-8

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