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Histomorphological Alterations in the Liver of Labeo rohita Exposed to Aflatoxin B1 and Quercetin-Enriched Diets

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Abstract

This study aimed to evaluate the effects of aflatoxin B1 and quercetin-enriched diets on histomorphological response of the liver, in freshwater fish Labeo rohita for 90 days. The fingerlings were divided into five groups (D1, control diet), AFB1 100 (D2), AFB1 25 QC (D3), AFB1 50 QC (D4), and AFB1 100 QC (D5). The study was carried out in fibre-reinforced plastic pools with three replicates for each group. After 90 days, fish from treatment were randomly caught, anaesthetized, and euthanized. Then, histological sections of the tissues were prepared. Results showed that fish exposed to 100 ppb AFB1 showed cytoplasmic vacuolization in hepatocytes, hepatic tissue showing loss of membrane integrity resulting in the cell fusion along with diffused hepatocytes, hyperplasia, blood congestion, and invasion of nuclei in the central vein. However, fish diet supplementation of D3 led to a significant improvement in the histomorphological responses of the liver.

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Acknowledgements

The authors express their gratitude to the Dean, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India, for providing the field and laboratory facilities to perform the research.

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Author notes

  1. Shanthanagouda A. Holeyappa

    Present address: Inland Fisheries Unit, Keladi Shivappa Nayaka University of Agricultural and Horticultural Sciences, NavuleShivamogga, 577 204, Karnataka, India

Authors and Affiliations

  1. Department of Aquaculture, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India

    Deepa Bhatt, Abhed Pandey & Sachin Onkar Khairnar

  2. Department of Aquatic Environment, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India

    Shanthanagouda A. Holeyappa

  3. Department of Veterinary Anatomy, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India

    Neelam Bansal

  4. Department of Aquaculture, College of Fisheries, Bihar Animal Sciences University, Patna, Arrabari, District-Kishanganj, 855107, Bihar, India

    Abhed Pandey & Sachin Onkar Khairnar

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  1. Deepa Bhatt
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Correspondence to Deepa Bhatt.

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Bhatt, D., Pandey, A., Holeyappa, S.A. et al. Histomorphological Alterations in the Liver of Labeo rohita Exposed to Aflatoxin B1 and Quercetin-Enriched Diets. Natl. Acad. Sci. Lett. (2023). https://doi.org/10.1007/s40009-023-01369-x

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