Skip to main content
SpringerLink
Log in

Effect of Physiological Age and Growth Regulators on Micropropagation of Aloe vera Followed by Genetic Stability Assessment

  • Research Article
  • Published:
National Academy Science Letters Aims and scope Submit manuscript

Abstract

Effect of physiological age and growth regulators on micropropagation of Aloe vera was investigated to develop an efficient protocol for in vitro propagation using shoot bud as explant. Maximum per cent establishment of 72.22 % was observed on MS medium supplemented with 10 µM BA, 5.0 µM Kn and 1.50 µM NAA. The best medium for the multiplication of microshoots was MS medium supplemented with 10 µM BA and 7.5 µM Kn followed by subculturing on same medium for six times at an interval of 4 weeks. Increase in number of microshoots per explant was observed with the increase in subculturing passages. After sixth subculturing 22.70 number of microshoots were obtained from the single explant which was four times more as compared to the first subculturing. The percent root induction was 77.78 % in the sixth subculturing as compared to 36.11 % in the first subculturing on MS medium supplemented with 0.04 % activated charcoal. Genetic stability of the in vitro grown plants was analysed by RAPD which indicated negligible variation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Tanabe M, Baerh S (2000) In vitro triple indexing of edible ginger (Zingiber officinale). J Hawaii Pac Agric 11:11–15

    Google Scholar 

  2. Morton JF (1961) Folk uses and commercial exploitation of Aloe leaf pulp. Econ Bot Bronx 15:311–319

    Article  Google Scholar 

  3. Mapp RK, McCarthy TJ (1970) The assessment of purgative principles in Aloes. Planta Med 18:361–365

    Article  Google Scholar 

  4. Abdi G, Hedayat M, Modarresi M (2013) In vitro micropropagation Aloe vera—impact of plant growth regulators, media and types of explant. J Biol Environ Sci 7(19):19–24

    Google Scholar 

  5. Sukamto LA (2011) Effect of physiological age and growth regulators on callus browning of coconut endosperm in vitro culture. Biotropica 18(1):31–45

    Google Scholar 

  6. Aggarawal D, Barna KS (2004) Tissue culture propagation of elite plant of Aloe vera. J Plant Biochem Biotechnol 13:77–79

    Article  Google Scholar 

  7. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small amount of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  8. Werner T, Motyka V, Strand M, Schmulling T (2001) Regulation of plant growth by cytokinin. PNAS 98(18):10487–10492

    Article  Google Scholar 

  9. Subotic A, Jevremovic S, Grubisic D (2009) Influence of cytokinin on in vitro morphogenesis in root cultures of Centaurium erythraea-valuable medicinal plant. Sci Hortic 120:386–390

    Article  Google Scholar 

  10. Hashemabadi D, Kaviani B (2008) Rapid micropropagation of Aloe vera L. via shoot multiplication. Afr J Biotechnol 7(12):1899–1902

    Google Scholar 

  11. Ahmed S, Kabir AH, Ahmed MB, Razvy MA, Ganesan S (2007) Development of rapid micropropagation method of Aloe vera L. Sjemenarstvo 24(2):121–128

    Google Scholar 

  12. Abrie A, Staden JV (2001) Micropropagation of endangered Aloe polyphylla. Plant Growth Regul 33(1):19–23

    Article  Google Scholar 

  13. Hosseini R, Parsa M (2007) Micropropagation of Aloe vera L. grown in South Iran. Pak J Biol Sci 10(7):1134–1137

    Article  Google Scholar 

  14. Thind SK, Jain N, Gosal SS (2008) Micropropagation of Aloe vera L. and estimation of potentially active secondary constitutes. Phytomorphology 58(1/2):65–71

    Google Scholar 

  15. Bhandari AK, Negi JS, Bisht VK, Bharti MK (2010) In vitro propagation of Aloe vera: plant with medicinal properties. Nat Sci 8(8):174–176

    Google Scholar 

  16. Deora NS, Shekhawat NS (1995) Micropropagation of Capparis deciduas (Forsk.) Edgew: tree of arid horticulture. Plant Cell Rep 15(3–4):278–281

    Google Scholar 

  17. Kanwar K, Josep J, Rahdeepika (2010) Comparison of in vitro regeneration pathways in Punica granatum L. Plant Cell Tiss Org 100:199–207

    Article  Google Scholar 

  18. Singh M, Rathore MS, Panwar D, Rathore JS, Dagla HR, Shekhawat NS (2009) Micropaopagation of selected genotype of Aloe vera L.: efficient plant for modern industry. J Sustain For 28(8):935–950

    Article  Google Scholar 

  19. Montoliu A, CadenasA G, Pérez-Clemente RM (2010) In vitro rooting of Carrizo citrange microshoots. HortScience 45(6):988–990

    Google Scholar 

  20. Soni M, Thakur M, Modgil M (2010) In vitro rooting of Merton I.793-an apple rootstock suitable for replantation. Indian J Biotechnol 10:362–368

    Google Scholar 

  21. Thakur M, Sharma DR, Kanwar K, Kant A (2002) In vitro regeneration of Acacia catechu Willd. from callus and mature nodal explants-an improved method. Indian J Exp Biol 40:850–853

    Google Scholar 

  22. Hussey G (1982) In vitro propagation of monocotyledonous bulbs and corms. In: Proceedings 5th International Plant Tissue, Cell Culture. pp 677–680

  23. Kanwar K, Deshmukh AJ, Rajdeepika, Kaushal B (2007) Efficient in vitro rapid axillary bud proliferation from mature Terminalia chebula Retz.- a medicinal tree. Tree For Sci Biotechnol 1(1):92–94

    Google Scholar 

  24. Rathore MS, Chikara J, Mastan SG, Rahman H, Anand KGV, Shekhawat NS (2011) Assessment of genetic stability and instability of tissue culture propagated plantlets of Aloe vera L. by RAPD and ISSR markers. Appl Biochem Biotechnol 165(5–6):1356–1365

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Dr. Y. S. Parmar University of Horticulture and Forestry, Solan, 173230, Himachal Pradesh, India

    Kamlesh Kanwar, Veena Devi, Swati Sharma, Madhvi Soni & Deepka Sharma

Authors
  1. Kamlesh Kanwar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Veena Devi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Swati Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Madhvi Soni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Deepka Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Madhvi Soni.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kanwar, K., Devi, V., Sharma, S. et al. Effect of Physiological Age and Growth Regulators on Micropropagation of Aloe vera Followed by Genetic Stability Assessment. Natl. Acad. Sci. Lett. 38, 29–35 (2015). https://doi.org/10.1007/s40009-014-0293-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40009-014-0293-9

Keywords

Search

Navigation