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Subcutaneous injection sites impact brain uptake of blood-brain barrier impermeable paclitaxel

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Abstract

Purpose

Systemic absorption is the process of drug movement from the site of drug administration to systemic circulation for therapeutic efficacy. Systemic absorption and distribution of therapeutics are affected by the route of administration and may further vary with different sites for the same route. Paclitaxel (PTX) remains one of the most powerful chemotherapeutic drugs to treat various tumors. Nevertheless, PTX has not been used to treat brain tumors because of its blood-brain barrier (BBB) impermeability and lack of drug accumulation in the brain. This study aimed to investigate systemic absorption and brain uptake of PTX following subcutaneous (SC) injection at different sites.

Methods

A hydroxypropyl methylcellulose (HPMC) polymer was used to prepare the PTX-HPMC hydrogel. The PTX-HPMC hydrogel was characterized by sol-gel transition, drug release, and in situ hydrogel formation. Systemic absorption and distribution in the brain were investigated in mice after subcutaneous injection at three different sites (flank, head, and abdomen). The PTX concentrations in the plasma and brain homogenates were quantified using LC-MS/MS using the liquid-liquid extraction method.

Results

The PTX-HPMC hydrogel showed sol-gel transition at 37 ℃ and bi-phase release with burst and sustained release. Systemic absorption in the plasma at all SC injection sites showed no significant differences. However, the distribution to the brain after SC injection at the head was 2.5 times higher than at the flank, suggesting an injection site-dependent distribution of PTX.

Conclusion

Differences in blood and lymph flow among SC sites are expected to contribute to PTX distribution. SC injection into the head may be a promising route for drug delivery to brain.

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF- 2020R1A2B5B01001719), Korea; National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Education) (NRF- 2021R1I1A1A01042149); and a National Research Foundation of Korea (NRF) grant funded by the Korea government (BK 21 four).

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Authors and Affiliations

  1. College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon, 21936, Republic of Korea

    O Hyun Lee, Ji Hee Kang & Young Tag Ko

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  1. O Hyun Lee
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  2. Ji Hee Kang
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  3. Young Tag Ko
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Correspondence to Young Tag Ko.

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Conflict of interest

All authors (O.H. Lee, J.H. Kang, and Y.T. Ko) declare that they have no competing financial interests or personal relationships that may have influenced the work reported in this study.

Human and animal rights

The animal study protocol was approved by the Institutional Animal Care and Use Committee at Gachon University (No. LCDI-2020-0062) and followed standard protocols for animal handling and care.

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Lee, O.H., Kang, J.H. & Ko, Y.T. Subcutaneous injection sites impact brain uptake of blood-brain barrier impermeable paclitaxel. J. Pharm. Investig. 53, 845–855 (2023). https://doi.org/10.1007/s40005-023-00634-x

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  • DOI: https://doi.org/10.1007/s40005-023-00634-x

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