In vivo pharmacokinetic applicability of a simple and validated HPLC method for orally administered trans-resveratrol loaded polymeric nanoparticles to rats

Abstract

A new formulation of trans-resveratrol nanoparticles (t-RVT NPs) with potential stealth properties was prepared by nano-precipitation method in our laboratory. To investigate the pharmacokinetic of t-RVT NPs, a simple and rapid high performance liquid chromatographic method was developed for the quantification of trans-resveratrol in plasma of rats treated with t-RVT NPs. Catechin (0.2 μg/ml) was used as an internal standard (IS). The chromatographic separation was achieved on a reversed-phase C₁₈ column using isocratic elution with methanol: 10 mM potassium dihydrogen phosphate buffer (pH 6.8): 3 % acetic acid solution (70:28:2, v/v/v) at a flow rate of 1 ml/min with a total run time of 6 min. The column effluent was monitored by UV detector at 306 nm. Calibration curve in plasma spiked with varying concentration of trans-resveratrol were linear over the concentration range of 0.010–3.2 μg/ml with correlation coefficient of (r² > 0.9987). The system was found to construct sharp peaks for trans-resveratrol and IS with retention times of 3.82 and 5.49 min, respectively. The method was sensitive with a limit of quantification of 0.007 μg/ml. The method showing accuracy, precision (inter-day relative standard deviation (RSD) and intra-day RSD values <15.0 %. The method was applied for determining trans-resveratrol concentration in plasma after oral administration of 20 mg/kg of free trans-resveratrol and t-RVT NPs to rats. The plasma concentrations of trans-resveratrol and t-RVT NPs at 30 min and 10 h after oral administration were quantified as 0.1771 ± 0.38 and 5.64 ± 0.436 μg/ml. Results established selectivity and suitability of the method for pharmacokinetic studies of trans-resveratrol from t-RVT NPs.