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Induced Effects of Host Plants on Susceptibility of Cotton Leafworm, Spodoptera littoralis to the Pathogenic Bacterium Bacillus thuringiensis subsp. Kurstaki and on Digestive Enzymatic Activities

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Abstract

The pathogen's effectiveness may be influenced by the insect's host plants. The purpose of this study was to examine if the entomopathogenic bacteria Bacillus thuringiensis var. kurstaki (BtK) was compatible with three hosts from diverse plant families including corn (Zea mays L.), lettuce (Lactuca sativa L.) and sugar beet (Beta vulgaris L.) against Spodoptera littoralis (Boisduval). BtK showed highest toxicity toward the sugar beet population compared with other host populations. The LC50 values of BtK were 17.67- and 7.89-fold for corn and sugar beet populations, respectively, lower than those of lettuce population. Because the difference might be explained by the dietary effects of host plants on the overall physiological condition of S. littoralis larvae, the digestive enzymatic and intermediate metabolism were investigated. The α-amylase and lipase activities in the midgut of S. littoralis lettuce population were all significantly higher than those of corn and sugar beet populations, while the highest activity of protease was observed in the corn population. In the lettuce population, the intermediary metabolism response, expressed as acid and alkaline phosphatases (ACP and ALP), was significantly (P ≤ 0.05) lower than those of the sugar beet and corn populations. On the contrary, the activity of lactate dehydrogenase (LDH) significantly increased on lettuce population compared with other host plants. The highest amount of protein (0.188 mg) and triglyceride (TAG: 0.219 mg/mL) was found in larvae fed on lettuce. According to these findings, the host plant has an indirect role in BtK's suppression of cotton leafworm.

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Abbreviations

BtK:

Bacillus thuringiensis Var. kurstaki

ACP and ALP:

Acid and alkaline phosphatases

LDH:

Lactate dehydrogenase

TAG:

Triglyceride

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  1. Central Agricultural Pesticides Laboratory, Insect Population Toxicology Department, Agriculture Research Center, Giza, 12618, Egypt

    Seham Mansour Ismail & Fatma Mohamed Amin Sleem

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  1. Seham Mansour Ismail
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  2. Fatma Mohamed Amin Sleem
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SMI subject selection, study design, carried out the experiments, paper writing, collecting, interpretation of the data, and performing statistical analysis. FMAS helped in statistical and chemical analysis. The authors read and approved the final manuscript.

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Correspondence to Seham Mansour Ismail.

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Ismail, S.M., Sleem, F.M.A. Induced Effects of Host Plants on Susceptibility of Cotton Leafworm, Spodoptera littoralis to the Pathogenic Bacterium Bacillus thuringiensis subsp. Kurstaki and on Digestive Enzymatic Activities. Agric Res 12, 172–178 (2023). https://doi.org/10.1007/s40003-022-00633-8

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