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Cross-Linking Methods of the Silk Protein Hydrogel in Oral and Craniomaxillofacial Tissue Regeneration

  • Review Article
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

BACKGROUND:

Craniomaxillofacial tissue defects are clinical defects involving craniomaxillofacial and oral soft and hard tissues. They are characterized by defect-shaped irregularities, bacterial and inflammatory environments, and the need for functional recovery. Conventional clinical treatments are currently unable to achieve regeneration of high-quality oral craniomaxillofacial tissue. As a natural biomaterial, silk fibroin (SF) has been widely studied in biomedicine and has broad prospects for use in tissue regeneration. Hydrogels made of SF showed excellent water retention, biocompatibility, safety and the ability to combine with other materials.

METHODS:

To gain an in-depth understanding of the current development of SF, this article reviews the structure, preparation and application prospects in oral and craniomaxillofacial tissue regenerative medicine. It first briefly introduces the structure of SF and then summarizes the principles, advantages and disadvantages of the different cross-linking methods (physical cross-linking, chemical cross-linking and double network structure) of SF. Finally, the existing research on the use of SF in tissue engineering and the prospects of using SF with different cross-linking methods in oral and craniomaxillofacial tissue regeneration are also discussed.

CONCLUSIONS:

This review is intended to show the advantages of SF hydrogels in tissue engineering and provides theoretical support for establishing novel and viable silk protein hydrogels for regeneration.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was financially supported by the Shanxi Key R&D Planning Program (202202130501009), Shanxi Provincial Medical Key Scientific Research Project (2022XM56), Basic Research Program of Shanxi Province project (202203021211225), Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (2020L0207) and Shanxi Medical University School and Hospital of Stomatology Program (KY201804 and KY201902).

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  1. Xiujuan Li and Yuanjiao Li have contributed equally to this work.

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  1. Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, 030001, Shanxi, China

    Xiujuan Li, Xinsong Zhang, Jie Xu, Jie Kang, Bing Li, Bin Zhao & Lu Wang

  2. School of Medical Sciences, Shanxi Medical University, Taiyuan, Shanxi, China

    Yuanjiao Li

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  1. Xiujuan Li
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Li, X., Li, Y., Zhang, X. et al. Cross-Linking Methods of the Silk Protein Hydrogel in Oral and Craniomaxillofacial Tissue Regeneration. Tissue Eng Regen Med 21, 529–544 (2024). https://doi.org/10.1007/s13770-023-00624-y

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