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Long and Short-Term Effect of mTOR Regulation on Cerebral Organoid Growth and Differentiations

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background

The mammalian target of rapamycin (mTOR) signaling is critical for the maintenance and differentiation of neurogenesis, and conceivably for many other brain developmental processes. However, in vivo studies of mTOR functions in the brain are often hampered due to the essential role of the associated signaling in brain development.

Methods

We monitored the long- and short-term effects of mTOR signaling regulation on cerebral organoids growth, differentiation and function using an mTOR inhibitor (everolimus) and an mTOR activator (MHY1485).

Results

Short-term treatment with MHY1485 induced faster organoid growth and differentiation, while long-term treatment induced the maturation of cerebral organoids.

Conclusion

These data suggest that the optimal activity of mTOR is crucial in maintaining normal brain development, and its role is not confined to the early neurogenic phase of brain development.

Graphical abstract

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors greatly acknowledge financial support from the Ministry of Science and ICT (2021R1A2C2011195), the Ministry of Trade, Industry & Energy (20009774), and the Korea Research Institute of Chemical Technology (SI2231-40, KK2252-10) of Republic of Korea.

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  1. Therapeutics and Biotechnology Division, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, Daejeon, 34114, Republic of Korea

    Sung Bum Park, Byungho Lim, Ki Young Kim & Byumseok Koh

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Correspondence to Ki Young Kim or Byumseok Koh.

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Park, S.B., Lim, B., Kim, K.Y. et al. Long and Short-Term Effect of mTOR Regulation on Cerebral Organoid Growth and Differentiations. Tissue Eng Regen Med 21, 159–169 (2024). https://doi.org/10.1007/s13770-023-00611-3

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  • DOI: https://doi.org/10.1007/s13770-023-00611-3

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