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Proliferation-Related Features of the Human Mesenchymal Stem Cells Derived from Palatine Tonsils, Adipose Tissues, and Bone Marrow

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Mesenchymal stem cells (MSCs) are widely used in regenerative medicine and cell-based transplantations. However, an in-depth comparison of the different MSC origins is lacking. This study aimed to compare the expression of adipose-derived (AMSCs), bone marrow-derived (BMSCs), and tonsil-derived (TMSCs) and evaluate whether TMSCs are good alternatives for AMSCs or BMSCs.

Methods:

We analyzed the expression levels of 47,000 transcripts in AMSCs (n = 4), BMSCs (n = 4), and TMSCs (n = 4) using GeneChip. Microarray data were analyzed using the LIMMA package to compare the TMSCs, AMSCs, and BMSCs. Hub genes were analyzed using STRING and Cytoscape. To ascertain the functional roles of AURKA and AURKB, small interfering RNA (siRNA) molecules specifically targeting AURKA and AURKB mRNA were synthesized and employed to induce knockdown of AURKA and AURKB in TMSC and AMSC. We analyzed the expression level of OCT4, SOX-2, and NANOG genes in TMSC and AMSCs by cell culture and real-time PCR.

Results:

We identified commonly increased 256 and decreased 160 genes in TMSCs from the differentially expressed genes (DEGs) between the TMSCs, AMSCs, and BMSCs. In the DEG-based protein–protein interaction and gene set enrichment analysis, hub genes (AURKA, AURKB, CDC20, and BUB1) highly expressed in TMSCs were enriched for development- and progression-related oocyte meiosis, the cell cycle, and ubiquitin-mediated proteolysis. In vitro analysis demonstrated that cells with downregulated expression of AURKA and AURKB exhibited a significant reduction in proliferation compared to control cells. However, silencing of the genes did not affect the differentiation capacity in TMSCs and AMSCs.

Conclusion:

Our study compared MSCs of different origins to better understand the similarities and differences among these cell types.

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Data availability

Microarray used in this study are available in the Gene Expression Omnibus (GEO) at https://www.ncbi.nlm.nih.gov/geo/, and can be accessed with GSE77272 (accession number).

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Acknowledgment

This study was supported by the Basic Research Program (No. 2021R1A6A3A01086785, 2022R1A2C2006697) of the National Research Foundation of Korea. This study was also supported in part by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), which is funded by the Ministry of Health & Welfare, Republic of Korea (No. HI22C1377).

Author information

Author notes

  1. Sohee Park and Yeuni Yu These authors contributed equally to this study.

Authors and Affiliations

  1. Convergence Medical Sciences, Pusan National University, Yangsan, 50612, Republic of Korea

    Sohee Park

  2. Biomedical Research Institute, School of Medicine, Pusan National University, Yangsan, 50612, Republic of Korea

    Yeuni Yu

  3. Department of Otolaryngology-Head and Neck Surgery, Samsung Changwon Hospital, Sungkyunkwan University College of Medicine, Changwon, Republic of Korea

    Gi Cheol Park

  4. Department of Otorhinolaryngology, College of Medicine, Pusan National University and Biomedical Research Institute, Pusan National University Hospital, 179 Gudeok-Ro, Busan, 49241, Republic of Korea

    Sung-Chan Shin & Byung-Joo Lee

  5. Pusan National University Medical Research Institute, College of Medicine, Pusan National University, Busan, Republic of Korea

    Ji Min Kim & Byung-Joo Lee

  6. Periodontal Disease Signaling Network Research Center, School of Dentistry, Pusan National University, Yangsan, 50612, Republic of Korea

    Yun Hak Kim

  7. Department of Anatomy and Department of Biomedical Informatics, School of Medicine, Pusan National University, 49 Busandaehak-Ro, Yangsan, 50612, Republic of Korea

    Yun Hak Kim

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Contributions

Sohee Park and Yeuni Yu contributed the manuscript writing, collection and/or assembly of data, data analysis and interpretation. Gi Cheol Park and Sung-Chan Shin contributed to the quality assessment of the study. Ji Min Kim and Byung-Joo Lee contributed to the designed the experiments. Yun Hak Kim and Byung-Joo Lee contributed to the study conception. Sohee Park, Yeuni Yu, Ji Min Kim, Byung-Joo Lee and Yun Hak Kim wrote the manuscript. All authors critically reviewed the manuscript and approved the final draft.

Corresponding authors

Correspondence to Byung-Joo Lee or Yun Hak Kim.

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Ethical Statement

This study was approved by the Institutional Review Board of Pusan National University Hospital (IRB No. 1801-033-062). Informed consent was confirmed by the IRB.

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Park, S., Yu, Y., Park, G.C. et al. Proliferation-Related Features of the Human Mesenchymal Stem Cells Derived from Palatine Tonsils, Adipose Tissues, and Bone Marrow. Tissue Eng Regen Med 20, 1119–1132 (2023). https://doi.org/10.1007/s13770-023-00564-7

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