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Induction of Salivary Gland-Like Tissue by Induced Pluripotent Stem Cells In Vitro

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

To investigate the in vitro induction of salivary gland-like tissue by ips cells in an interferon regulatory factor 6 (IRF6) overexpression and parotid conditioned medium environment.

Methods:

Urine-derived ips cells were isolated, identified, transfected with IRF6 and cultured in parotid conditioned medium to induce ips cells into salivary gland differentiation, morphological changes of ips cells were observed, CCK-8 was used to determine the cell proliferation efficiency and transcriptome sequencing was used to detect the expression of genes related to parotid gland formation.

Results:

Immunofluorescence staining showed that the isolated ips cells were positive for NANOG, SSEA4 and OCT4 and had embryonic-like stem cell characteristics; CCK-8 showed that there was no statistical difference in the proliferation efficiency between the IRF6+ induced group and the simple induced group after induction of ips cells into salivary glands. The results of transcriptome sequencing showed that there were a total of 643 differentially expressed genes, including 365 up-regulated genes and 278 down-regulated genes in the IRF6+ induced group compared to the blank control group, and the salivary gland related genes HAPLN1, CCL2, MSX2, ANXA1, CYP11A1, HES1 and LUM were all highly expressed in the IRF6+ induced group.

Conclusion:

IRF6 promotes salivary gland differentiation in urine-derived iPSCs, and its mechanism of promoting differentiation may be that IRF6 upregulates the expression of HAPLN1, CCL2, MSX2, ANXA1, CYP11A1, HES1 and LUM to promote epithelial differentiation.

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Acknowledgement

This work was supported by the National Science Foundation of China (no. 81873718).

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Author notes

  1. Cen Meng and Shengyuan Huang contributed equally to this study.

Authors and Affiliations

  1. Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing, China

    Cen Meng, Taiqi Cheng, Xue Zhang & Xing Yan

  2. Department of Stomatology, Beijing Tongren Hospital, Capital Medical University, Beijing, China

    Shengyuan Huang

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  1. Cen Meng
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  2. Shengyuan Huang
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  3. Taiqi Cheng
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  4. Xue Zhang
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  5. Xing Yan
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Correspondence to Xing Yan.

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All of the authors had no any personal, financial, commercial, or academic conflicts of interest separately.

Ethical statement

This study was conducted in accordance with the Declaration of Helsinki and approved by the ethics committee of our Hospital (IRB no. 2020-P2-209-02).

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Meng, C., Huang, S., Cheng, T. et al. Induction of Salivary Gland-Like Tissue by Induced Pluripotent Stem Cells In Vitro. Tissue Eng Regen Med 19, 389–401 (2022). https://doi.org/10.1007/s13770-021-00402-8

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  • DOI: https://doi.org/10.1007/s13770-021-00402-8

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