Abstract
Background:
Silica particles (SPs) induce cell proliferation and osteogenic differentiation. We reported that SPs in the scaffold induced early stage osteogenic differentiation.
Methods:
A polycaprolactone (PCL) scaffold was fabricated with a 10 wt% SPs. The surface of PCL scaffold was coated with a 10 µg/mL collagen solution. Next, the scaffold was conjugated with 2 μM SPs, 2 μg/mL bone morphogenetic protein 2 (BMP2), or 2 μM BMP2-conjugated SPs (BCSPs). Green fluorescent protein-coupled BMP2 was applied to fabricate the scaffold. The fluorescence intensity was analyzed by confocal microscopy. The mRNA levels of the early osteogenic differentiation marker, alkaline phosphatase (ALP), were analyzed by real-time quantitative polymerase chain reaction. Levels of BMP2, RUNX2, ERK1/2, and AKT were assessed by western blotting.
Results:
ALP mRNA levels were significantly higher in the BCSP-conjugated scaffold than in the other scaffolds. In the early stage of osteogenic differentiation, the protein levels of BMP2, RUNX2, ERK1/2, and AKT in cells were significantly higher in the BCSP-conjugated scaffold than in other scaffolds. Thus, the BCSP composite scaffold induced rapid osteogenic differentiation.
Conclusion:
These results suggest that BCSP composite can be used to promote early stage osteogenic differentiation and show promise as a material for use in scaffolds for bone regeneration.
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Acknowledgements
This work was financially supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1A6A3A11030383 and 2017M3A9E2060428).
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The study protocol was approved by the institutional review board of Seoul St. Mary’s Hospital, Republic of Korea (IRB No. KC17TESI0546).
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Fig. S1
Fabrication of the scaffold coatings. (A) Fabrication process of the SP-conjugated scaffold. (B) Fabrication process of the BCSP-conjugated scaffold. (C) Fabrication process of the BMP2-conjugated scaffold. (JPEG 2760 kb)
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Kim, K.J., Choi, M.S., Shim, J.H. et al. Bone Morphogenetic Protein 2-Conjugated Silica Particles Enhanced Early Osteogenic Differentiation of Adipose Stem Cells on the Polycaprolactone Scaffold. Tissue Eng Regen Med 16, 395–403 (2019). https://doi.org/10.1007/s13770-019-00195-x
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DOI: https://doi.org/10.1007/s13770-019-00195-x