Abstract
Urine-derived stem cells (USCs) are considered as a promising cell source capable of neuronal differentiation. In addition, specific growth factors and extracellular matrix are essential for enhancing their neuronal differentiation efficiency. In this study, we investigated the possibility of neuronal differentiation of USCs and the role of laminin and platelet-derived growth factor BB (PDGF-BB) as promoting factors. USCs were isolated from fresh urine of healthy donors. Cultured USCs were adherent to the plate and their morphology was similar to the cobblestone. In addition, they showed chromosome stability, rapid proliferation rate, colony forming capacity, and mesenchymal stem cell characteristics. For inducing the neuronal differentiation, USCs were cultured for 14 days in neuronal differentiation media supplemented with/without laminin and/or PDGF-BB. To identify the expression of neuronal markers, RT-PCR, flow cytometry analysis and immunocytochemistry were used. After neuronal induction, the cells showed neuron-like morphological change and high expression level of neuronal markers. In addition, laminin and PDGF-BB respectively promoted the neuronal differentiation of USCs and the combination of laminin and PDGF-BB showed a synergistic effect for the neuronal differentiation of USCs. In conclusion, USCs are noteworthy cell source in the field of neuronal regeneration and laminin and PDGF-BB promote their neuronal differentiation efficiency.
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Acknowledgements
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), which is funded by the Ministry of Science, ICT and Future Planning (2014M3A9D3034164) (2014R1A1A3049460). Additional funding was provided by the Korean government (MSIP) (2015R1C1A1A01053509), (2016R1C1B1011180) and the Ministry of Education (2015R1D1A3A03020378).
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Ethical Statement
This study was approved by the Ethics Committee of the Kyungpook National University School of Medicine (No. KNUMC 2016-05-021). This study was performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.
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Kim, J.Y., Chun, S.Y., Park, JS. et al. Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells. Tissue Eng Regen Med 15, 195–209 (2018). https://doi.org/10.1007/s13770-017-0102-x
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DOI: https://doi.org/10.1007/s13770-017-0102-x