Tissue Engineering and Regenerative Medicine

, Volume 15, Issue 2, pp 195–209 | Cite as

Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells

  • Jung Yeon Kim
  • So Young Chun
  • Jin-Sung Park
  • Jae-Wook Chung
  • Yun-Sok Ha
  • Jun Nyung Lee
  • Tae Gyun Kwon
Original Article


Urine-derived stem cells (USCs) are considered as a promising cell source capable of neuronal differentiation. In addition, specific growth factors and extracellular matrix are essential for enhancing their neuronal differentiation efficiency. In this study, we investigated the possibility of neuronal differentiation of USCs and the role of laminin and platelet-derived growth factor BB (PDGF-BB) as promoting factors. USCs were isolated from fresh urine of healthy donors. Cultured USCs were adherent to the plate and their morphology was similar to the cobblestone. In addition, they showed chromosome stability, rapid proliferation rate, colony forming capacity, and mesenchymal stem cell characteristics. For inducing the neuronal differentiation, USCs were cultured for 14 days in neuronal differentiation media supplemented with/without laminin and/or PDGF-BB. To identify the expression of neuronal markers, RT-PCR, flow cytometry analysis and immunocytochemistry were used. After neuronal induction, the cells showed neuron-like morphological change and high expression level of neuronal markers. In addition, laminin and PDGF-BB respectively promoted the neuronal differentiation of USCs and the combination of laminin and PDGF-BB showed a synergistic effect for the neuronal differentiation of USCs. In conclusion, USCs are noteworthy cell source in the field of neuronal regeneration and laminin and PDGF-BB promote their neuronal differentiation efficiency.


Urine-derived stem cells Laminin Platelet-derived growth factor-BB Neuronal differentiation Mesenchymal stem cell source 



This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), which is funded by the Ministry of Science, ICT and Future Planning (2014M3A9D3034164) (2014R1A1A3049460). Additional funding was provided by the Korean government (MSIP) (2015R1C1A1A01053509), (2016R1C1B1011180) and the Ministry of Education (2015R1D1A3A03020378).

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical Statement

This study was approved by the Ethics Committee of the Kyungpook National University School of Medicine (No. KNUMC 2016-05-021). This study was performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.


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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Jung Yeon Kim
    • 1
  • So Young Chun
    • 1
  • Jin-Sung Park
    • 2
  • Jae-Wook Chung
    • 3
  • Yun-Sok Ha
    • 3
  • Jun Nyung Lee
    • 3
  • Tae Gyun Kwon
    • 1
    • 3
  1. 1.Joint Institute for Regenerative MedicineKyungpook National University HospitalDaeguKorea
  2. 2.Department of Neurology, School of MedicineKyungpook National UniversityDaeguKorea
  3. 3.Department of Urology, School of MedicineKyungpook National UniversityDaeguKorea

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