Abstract
The aim of this study was to investigate the synergistic effect of cold atmospheric plasma (CAP) treatment and RGD peptide coating for enhancing cellular attachment and proliferation over titanium (Ti) surfaces. The surface structure of CAP-treated and RGD peptide-coated Ti discs were characterized by contact angle goniometer and atomic force microscopy. The effect of such surface modification on human bone marrow derived mesenchymal stem cells (hMSCs) adhesion and proliferation was assessed by cell proliferation and DNA content assays. Besides, hMSCs’ adhesion and morphology on surface modified Ti discs were observed via fluorescent and scanning electron microscopy. RGD peptide coating following CAP treatment significantly enhanced cellular adhesion and proliferation among untreated, CAP-treated and RGD peptide-coated Ti discs. The treatment of Ti surfaces with CAP may contribute to improved RGD peptide coating, which enables increased cellular integrations with the Ti surfaces.
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Acknowledgements
Authors acknowledge funding from TÜBİTAK (The Scientific and Technological Research Council of Turkey) through the Research Project 214M268 and BAP (Scientific Research Projects Fund of Izmir Katip Çelebi University) through the Research Projects 2015-TDR-SABE-0012 and 2016-ONP-MUMF-0022. Authors also acknowledge Dr. Mustafa Can, Eyup Yalçın and Dr. Nesrin Horzum Polat (Department of Engineering Sciences, Izmir Katip Çelebi University) for their assistance on AFM and contact angle measurements, respectively. Finally authors would like to acknowledge Titania Medical Devices (Izmir, Turkey) and Bonegraft Biological Materials A.Ş. (Izmir, Turkey) for providing the Ti discs and DNA quantification kit, respectively.
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Karaman, O., Kelebek, S., Demirci, E.A. et al. Synergistic Effect of Cold Plasma Treatment and RGD Peptide Coating on Cell Proliferation over Titanium Surfaces. Tissue Eng Regen Med 15, 13–24 (2018). https://doi.org/10.1007/s13770-017-0087-5
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DOI: https://doi.org/10.1007/s13770-017-0087-5