Abstract
The repair of bone defects poses a great challenge for reconstructive surgeons. Although the development of tißsue engineering has exhibited promise in replacing damaged bone, the fabrication of large constructs with functional blood veßsels remains an obstacle. From the orthopedic surgeon’s point of view, the generation of axially vascularized bone, which can anastomose with the recipient vessel, might be a solution to this medical problem. In this study, we aimed to prefabricate an axially vascularized bone by combining a ß-TCP scaffold, arteriovenous loop (AVL), and cell sheet in a bioreactor in vivo. Twelve rabbits were randomly allocated into two groups: the experimental group (presence of AVL) and the control group (absence of AVL). The constructs were explanted at 8 weeks postoperatively. The histomorphometric results showed 42.8±5.9% of the bone area in the AVL group and 26.6±3.5% in the control group. Similarly, vessel analysis revealed the average vessel density in the AVL group (12.5±3.3) was significantly more than that in the control group (6.1±1.5, p<0.05). Our research indicated that the combination of a ß-TCP scaffold, AVL and cell sheet might engineer vascularized bone. This prefabrication strategy might facilitate clinical translation of bone tissue engineering in reconstructing large bone defects.
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References
Roux BM, Cheng MH, Brey EM. Engineering clinically relevant volumes of vascularized bone. J Cell Mol Med 2015;19:903–914.
Weigand A, Beier JP, Hess A, Gerber T, Arkudas A, Horch RE, et al. Acceleration of vascularized bone tissue-engineered constructs in a large animal model combining intrinsic and extrinsic vascularization. Tissue Eng Part A 2015;21:1680–1694.
Nguyen LH, Annabi N, Nikkhah M, Bae H, Binan L, Park S, et al. Vascularized bone tissue engineering:approaches for potential improvement. Tissue Eng Part B Rev 2012;18:363–382.
Kneser U, Polykandriotis E, Ohnolz J, Heidner K, Grabinger L, Euler S, et al. Engineering of vascularized transplantable bone tissues:induction of axial vascularization in an osteoconductive matrix using an arteriovenous loop. Tissue Eng 2006;12:1721–1731.
Ma D, Yao H, Tian W, Chen F, Liu Y, Mao T, et al. Enhancing bone formation by transplantation of a scaffold-free tissue-engineered periosteum in a rabbit model. Clin Oral Implants Res 2011;22:1193–1199.
Kuo KC, Lin RZ, Tien HW, Wu PY, Li YC, Melero-Martin JM, et al. Bioengineering vascularized tissue constructs using an injectable cell-laden enzymatically crosslinked collagen hydrogel derived from dermal extracellular matrix. Acta Biomater 2015;27:151–166.
Arkudas A, Beier JP, Heidner K, Tjiawi J, Polykandriotis E, Srour S, et al. Axial prevascularization of porous matrices using an arteriovenous loop promotes survival and differentiation of transplanted autologous osteoblasts. Tissue Eng 2007;13:1549–1560.
Ma D, Zhong C, Yao H, Liu Y, Chen F, Li J, et al. Engineering injectable bone using bone marrow stromal cell aggregates. Stem Cells Dev 2011;20:989–999.
Murphy CM, O’Brien FJ, Little DG, Schindeler A. Cell-scaffold interactions in the bone tissue engineering triad. Eur Cell Mater 2013;26:120–132.
Stegen S, van Gastel N, Carmeliet G. Bringing new life to damaged bone:the importance of angiogenesis in bone repair and regeneration. Bone 2015;70:19–27.
Warnke PH, Springer IN, Wiltfang J, Acil Y, Eufinger H, Wehmöller M, et al. Growth and transplantation of a custom vascularised bone graft in a man. Lancet 2004;364:766–770.
Erol OO, Sira M. New capillary bed formation with a surgically constructed arteriovenous fistula. Plast Reconstr Surg 1980;66:109–115.
Horch RE, Beier JP, Kneser U, Arkudas A. Successful human long-term application of in situ bone tissue engineering. J Cell Mol Med 2014;18:1478–1485.
Beier JP, Hess A, Loew J, Heinrich J, Boos AM, Arkudas A, et al. De novo generation of an axially vascularized processed bovine cancellousbone substitute in the sheep arteriovenous-loop model. Eur Surg Res 2011;46:148–155.
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Ma, D., Ren, L., Cao, Z. et al. Prefabrication of axially vascularized bone by combining β-tricalciumphosphate, arteriovenous loop, and cell sheet technique. Tissue Eng Regen Med 13, 579–584 (2016). https://doi.org/10.1007/s13770-016-9095-0
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DOI: https://doi.org/10.1007/s13770-016-9095-0