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The effect of platelet-rich fibrin on bone regeneration and angiogenesis in rabbit cranial defects

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

This study evaluated the effect of platelet-rich fibrin (PRF) on the osteogenesis and angiogenesis of rabbit calvarial defects. The calvarium of nine rabbits was exposed. In each rabbit, two circular bone defects, one on each side of the midline, were prepared using a reamer drill. While the experimental site received PRF, the control sites were left filled with blood. The animals were sacrificed after a period of 1 week (n=3), 2 weeks (n=3), and 4 weeks (n=3). The following histomorphometric data were recorded: 1) the area of the newly formed tissue relative to total bone defect (NT) and the area of newly formed osteoid relative to newly formed tissue (NB), 2) the longest and shortest vertical height of the newly formed tissue relative to the vertical height of normal tissue (LH, SH), 3) newly formed osteoid on the defect margin using region of interest (NBR), and 4) bony pontic formation from the margin was used to evaluate bone growth (OS). The expression of vascular endothelial growth factor (VEGF) was evaluated by immunohistochemical staining. The differences between groups were not significant at any experimental time point. However, a significant linear increase of NB and NBR including OS value according to time was observed in the PRF group. In addition, the intensity and percentage range of VEGF expression showed a significant linear decrease trend with the time in the PRF group. The results of this study suggest that PRF may enhance the quality of bone regeneration.

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References

  1. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:638–646.

    Article  CAS  PubMed  Google Scholar 

  2. Su CY, Kuo YP, Tseng YH, Su CH, Burnouf T. In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:56–61.

    Article  PubMed  Google Scholar 

  3. Kim SG, Chung CH, Kim YK, Park JC, Lim SC. Use of particulate dentin-plaster of Paris combination with/without platelet-rich plasma in the treatment of bone defects around implants. Int J Oral Maxillofac Implants 2002;17:86–94.

    PubMed  Google Scholar 

  4. DurmuSlar MC, Alpaslan C, Alpaslan G, Ç akir M. Clinical and radiographic evaluation of the efficacy of platelet-rich plasma combined with hydroxyapatite bone graft substitutes in the treatment of intra-bony defects in maxillofacial region. Acta Odontol Scand 2014;72:948–953.

    Article  CAS  PubMed  Google Scholar 

  5. Dohan Ehrenfest DM, de Peppo GM, Doglioli P, Sammartino G. Slow release of growth factors and thrombospondin-1 in Choukroun’s platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors 2009;27:63–69.

    Article  CAS  PubMed  Google Scholar 

  6. Thor A, Franke-Stenport V, Johansson CB, Rasmusson L. Early bone formation in human bone grafts treated with platelet-rich plasma: preliminary histomorphometric results. Int J Oral Maxillofac Surg 2007;36: 1164–1171.

    Article  CAS  PubMed  Google Scholar 

  7. Jung RE, Schmoekel HG, Zwahlen R, Kokovic V, Hammerle CH, Weber FE. Platelet-rich plasma and fibrin as delivery systems for recombinant human bone morphogenetic protein-2. Clin Oral Implants Res 2005;16: 676–682.

    Article  PubMed  Google Scholar 

  8. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e37–e44.

    Article  PubMed  Google Scholar 

  9. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e45–e50.

    Article  PubMed  Google Scholar 

  10. Dohan Ehrenfest DM, Bielecki T, Jimbo R, Barbé G, Del Corso M, Inchingolo F, et al. Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a pure platelet-rich plasma (P-PRP) gel and a leukocyte- and platelet-rich fibrin (L-PRF). Curr Pharm Biotechnol 2012;13: 1145–1152.

    Article  PubMed  Google Scholar 

  11. Clark RA. Fibrin and wound healing. Ann N YAcad Sci 2001;936:355–367.

    Article  CAS  Google Scholar 

  12. Hom DB, Linzie BM, Huang TC. The healing effects of autologous platelet gel on acute human skin wounds. Arch Facial Plast Surg 2007;9:174–183.

    Article  PubMed  Google Scholar 

  13. Lundquist R, Dziegiel MH, Agren MS. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin. Wound Repair Regen 2008;16:356–363.

    Article  PubMed  Google Scholar 

  14. Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e56–e60.

    Article  PubMed  Google Scholar 

  15. Yoon JS, Lee SH, Yoon HJ. The influence of platelet-rich fibrin on angiogenesis in guided bone regeneration using xenogenic bone substitutes: a study of rabbit cranial defects. J Craniomaxillofac Surg 2014;42:1071–1077.

    Article  PubMed  Google Scholar 

  16. Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299–303.

    Article  PubMed  Google Scholar 

  17. Sunitha Raja V, Munirathnam Naidu E. Platelet-rich fibrin: evolution of a second-generation platelet concentrate. Indian J Dent Res 2008;19:42–46.

    Article  CAS  PubMed  Google Scholar 

  18. Lacoste E, Martineau I, Gagnon G. Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release. J Periodontol 2003;74:1498–1507.

    Article  CAS  PubMed  Google Scholar 

  19. Li Q, Pan S, Dangaria SJ, Gopinathan G, Kolokythas A, Chu S, et al. Platelet-rich fibrin promotes periodontal regeneration and enhances alveolar bone augmentation. Biomed Res Int 2013;2013:638043.

    PubMed Central  PubMed  Google Scholar 

  20. He L, Lin Y, Hu X, Zhang Y, Wu H. A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:707–713.

    Article  PubMed  Google Scholar 

  21. Pripatnanont P, Nuntanaranont T, Vongvatcharanon S, Phurisat K. The primacy of platelet-rich fibrin on bone regeneration of various grafts in rabbit’s calvarial defects. J Craniomaxillofac Surg 2013;41:e191–e200.

    Article  PubMed  Google Scholar 

  22. Ozdemir H, Ezirganli S, Isa Kara M, Mihmanli A, Baris E. Effects of platelet rich fibrin alone used with rigid titanium barrier. Arch Oral Biol 2013;58:537–544.

    Article  CAS  PubMed  Google Scholar 

  23. Lee EH, Kim JY, Kweon HY, Jo YY, Min SK, Park YW, et al. A combination graft of low-molecular-weight silk fibroin with Choukroun plateletrich fibrin for rabbit calvarial defect. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:e33–e38.

    Article  PubMed  Google Scholar 

  24. Panda S, Doraiswamy J, Malaiappan S, Varghese SS, Del Fabbro M. Additive effect of autologous platelet concentrates in treatment of intrabony defects: a systematic review and meta-analysis. J Investig Clin Dent 2014 Jul 22 [Epub]. http://dx.doi.org/10.1111/jicd.12117.

  25. Uchida S, Sakai A, Kudo H, Otomo H, Watanuki M, Tanaka M, et al. Vascular endothelial growth factor is expressed along with its receptors during the healing process of bone and bone marrow after drill-hole injury in rats. Bone 2003;32:491–501.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, St. Paul’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea

    Sang-Hwa Lee

  2. Private Practice, Seoul, Korea

    Sun-Woo Kim

  3. Department of Oral and Maxillofacial Surgery, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea

    Jae-Il Lee

  4. Department of Oral and Maxillofacial Surgery, Bucheon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Bucheon, Korea

    Hyun-Joong Yoon

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  1. Sang-Hwa Lee
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  2. Sun-Woo Kim
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  3. Jae-Il Lee
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  4. Hyun-Joong Yoon
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Correspondence to Hyun-Joong Yoon.

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Lee, SH., Kim, SW., Lee, JI. et al. The effect of platelet-rich fibrin on bone regeneration and angiogenesis in rabbit cranial defects. Tissue Eng Regen Med 12, 362–370 (2015). https://doi.org/10.1007/s13770-015-0031-5

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  • DOI: https://doi.org/10.1007/s13770-015-0031-5

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